[1]李晶,董荣娜,于德民.有氧运动对胰岛素抵抗小鼠visfatin 表达的影响[J].国际内分泌代谢杂志,2016,36(01):10-13,31.[doi:10.3760/cma.j.issn.1673-4157.2016.01.003]
 Li Jing,Dong Rongna,Yu Demin..Effects of aerobic exercise on the expression of visfatin in mice with insulin resistance[J].International Journal of Endocrinology and Metabolism,2016,36(01):10-13,31.[doi:10.3760/cma.j.issn.1673-4157.2016.01.003]
点击复制

有氧运动对胰岛素抵抗小鼠visfatin 表达的影响()
分享到:

《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
36
期数:
2016年01期
页码:
10-13,31
栏目:
论著
出版日期:
2016-01-20

文章信息/Info

Title:
Effects of aerobic exercise on the expression of visfatin in mice with insulin resistance
作者:
李晶董荣娜于德民
300070 天津医科大学代谢病医院内分泌研究所,卫生部激素与发育重点实验室
Author(s):
Li Jing Dong RongnaYu Demin.
Key Laboratory of Hormones and Development(Ministry of Health),The Metabolic Disease Hospital & Tianjin Institute of Endocrinology,Tianjin Medical University,Tianjin 300070,China
关键词:
有氧运动 胰岛素抵抗 内脂素 脂代谢
Keywords:
Aerobic exercise Insulin resistance Visfatin Lipid metabolism
DOI:
10.3760/cma.j.issn.1673-4157.2016.01.003
摘要:
目的 探讨高脂饮食诱导的胰岛素抵抗(IR)小鼠visfatin表达的改变及有氧运动对visfatin表达及脂代谢的影响。方法 雄性C57BL/6小鼠24只,按随机数字表随机分为普通饮食组(n=12)和高脂饮食组(n=12),后者喂养8周高脂饲料建立IR模型,再各自分为普通饮食对照组(n=6)、普通饮食运动组(n=6)、高脂饮食对照组(n=6)、高脂饮食运动组(n=6)。两个运动组进行6周跑台训练,各组饮食不变。14周后ELISA检测小鼠血清visfatin,Western印迹检测腓肠肌、肝脏及睾周脂肪visfatin表达。结果 8周后,与普通饮食组相比,高脂饮食组空腹胰岛素水平升高,血糖曲线下面积增加(t=6.32,17.44,P均<0.01)。14周后,高脂饮食运动组血清visfatin水平低于高脂饮食对照组,而普通饮食运动组血清visfatin水平高于普通饮食对照组(F=43.59,P<0.05)。高脂饮食运动组骨骼肌、肝脏、内脏脂肪组织中visfatin 蛋白表达水平较高脂饮食对照组降低(F=29.33~85.17,P均<0.05),普通饮食运动组骨骼肌visfatin表达较普通饮食对照组升高(F=52.79,P<0.05),但肝脏、内脏脂肪组织visfatin蛋白表达在普通饮食组间无明显变化(P均>0.05)。结论 高脂饮食可以增加IR模型动物visfatin水平,有氧运动能有效下调visfatin的表达,改善脂代谢紊乱。
Abstract:
Objective To explore the expression of visfatin in mice with insulin resistance(IR)induced by high-fat diet and the effects of aerobic exercise on the expression of visfatin and lipid metabolism. Methods A total of 24 male C57BL/6 mice were randomly divided into normal diet group(n=12)and high-fat diet group(n=12)according to the table of random number. Mice in high-fat diet group were fed with high-fat diet for 8 weeks to induce IR. Both groups were divided into normal diet control group(n=6),normal diet exercise group(n=6)and high-fat diet control group(n=6),high-fat diet exercise group(n=6),respectively.Mice in both exercise groups underwent treadmill exercise for 6 weeks. All mice did not change diet. After 14 weeks, serum visfatin concentration was evaluated by ELISA. And Western blotting was applied to detect the expression of visfatin in gastrocnemius muscle, liver and epididymal tissue. Results Eight weeks later, compared with normal diet group, fasting insulin of high-fat diet group was elevated, and blood glucose area under curve was increased(t=6.32,17.44,all P<0.01). Fourteen weeks later, the level of serum visfatin in high-fat diet exercise group was lower than that in high-fat diet control group, but the level of serum visfatin in normal diet exercise group was higher than that in normal diet control group(F=43.59,P<0.05). Compared with high-fat diet control group, the expression of visfatin in skeletal muscle, liver and adipose tissue were reduced in high-fat diet exercise group(F=29.33-85.17, all P<0.05). On the contrary,the expression of visfatin in skeletal muscle in normal diet exercise group was higher than that in normal diet control group(F=52.79, P<0.05), but there were no differences in the expression of visfatin in liver and adipose tissue between normal diet groups(all P>0.05).Conclusion High-fat diet increases the level of visfatin, whereas aerobic exercise reduces the expression of visfatin and improves lipid metabolism in IR models.

参考文献/References:

[1] Kurose S,Tsutsumi H,Yamanaka Y,et al. Improvement in endothelial function by lifestyle modification focused on exercise training is associated withinsulin resistance in obese patients[J].Obes Res Clin Pract,2014,8(1):e106-e114. DOI: 10.1016/j.orcp.2012.10.005.
[2]Koceak P,Olszanecka-Glinianowicz M,Owczarek AJ,et al. Plasma visfatin/nicotinamide phosphoribosyltransferase(visfatin/NAMPT)concentration in elderly subjects withmetabolic syndrome[J].Pol Arch Med Wewn,2015,125(6):402-413.
[3] Dogru T,Sonmez A,Tasci I,et al. Plasma visfatin levels in patients with newly diagnosed and untreated type 2 diabetes mellitus and impairedglucose tolerance[J].Diabetes Res Clin Pract,2007,76(1):24-29.
[4] Xiang RL,Mei M,Su YC,et al. Visfatin protects rat pancreatic β-cells against IFN-γ-induced apoptosis through AMPK and ERK1/2 signaling pathways[J].Biomed Environ Sci,2015,28(3):169-177. DOI:10.3967/bes2015.023.
[5]Fukuhara A,Matsuda M,Nishizawa M,et al. Visfatin: a protein secreted by visceral fat that mimics the effects of insulin[J].Science,2005,307(5708):426-430.
[6]Wang T,Zhang X,Bheda P,et al. Structure of Nampt/PBEF/visfatin, a mammalian NAD+ biosynthetic enzyme[J].Nat Struct Mol Biol,2006,13(7):661-662.
[7]Jacques C,Holzenberger M,Mladenovic Z,et al. Proinflammatory actions of visfatin/nicotinamide phosphoribosyltransferase(Nampt)involve regulation of insulinsignaling pathway and Nampt enzymatic activity[J].J Biol Chem,2012,287(18):15100-15108. DOI:10.1074/jbc.M112.350215.
[8] Stofkova A. Resistin and visfatin: regulators of insulin sensitivity, inflammation and immunity[J].Endocr Regul,2010,44(1):25-36.
[9] Trevellin E,Scorzeto M,Olivieri M,et al. Exercise training induces mitochondrial biogenesis and glucose uptake in subcutaneous adipose tissue through eNOS-dependent mechanisms[J].Diabetes,2014,63(8):2800-2811. DOI:10.2337/db13-1234.
[10] Liu X,Niu Y,Yuan H,et al. AMPK binds to Sestrins and mediates the effect of exercise to increase insulin-sensitivity through autophagy[J].Metabolism,2015,64(6):658-665.DOI:10.1016/j.metabol.2015.01.015.
[11] Li J,Zhang W,Guo Q,et al. Duration of exercise as a key determinant of improvement in insulin sensitivity in type 2 diabetes patients[J].Tohoku J Exp Med,2012,227(4):289-296.

相似文献/References:

[1]刘丹丹,王丽,史兴晔,等.脂肪酸受体GPR120影响胰岛素受体底物-1的表达[J].国际内分泌代谢杂志,2014,(06):371.[doi:10.3760/cma.j.issn.1673-4157.2014.06.003]
 Liu Dandan*,Wang Li,Shi Xingye,et al.Regulation of fatty acid receptor GPR120 on the expression of insulin receptor substrate-1[J].International Journal of Endocrinology and Metabolism,2014,(01):371.[doi:10.3760/cma.j.issn.1673-4157.2014.06.003]
[2]李新萍,张萍,李晓玉.螺内酯对特发性醛固酮增多症患者胰岛β细胞功能的影响[J].国际内分泌代谢杂志,2014,(06):379.[doi:10.3760/cma.j.issn.1673-4157.2014.06.005]
 Li Xinping,Zhang Ping,Li Xiaoyu..Effects of spironolactone on islet β cell function in patients with idiopathic hyperaldosteronism[J].International Journal of Endocrinology and Metabolism,2014,(01):379.[doi:10.3760/cma.j.issn.1673-4157.2014.06.005]
[3]胡雅琴,包玉倩.维生素D对非酒精性脂肪性肝病的保护作用[J].国际内分泌代谢杂志,2014,(06):408.[doi:10.3760/cma.j.issn.1673-4157.2014.06.013]
 Hu Yaqin,Bao Yuqian..Protective effect of vitamin D on non-alcoholic fatty liver disease[J].International Journal of Endocrinology and Metabolism,2014,(01):408.[doi:10.3760/cma.j.issn.1673-4157.2014.06.013]
[4]张琳,王晓琳,李小英.雄激素与糖脂代谢的关系[J].国际内分泌代谢杂志,2014,(05):351.[doi:10.3760/cma.j.issn.1673-4157.2014.05.018]
 Zhang Lin,Wang Xiaolin,Li Xiaoying..The relationship between testosterone and glycolipid,metabolism[J].International Journal of Endocrinology and Metabolism,2014,(01):351.[doi:10.3760/cma.j.issn.1673-4157.2014.05.018]
[5]梁绮君 余寿益 李淑华 胡晨鸣 杨焱.甲状腺结节与代谢综合征的相关性研究[J].国际内分泌代谢杂志,2015,(05):293.[doi:DOI:10.3760/cma.j.issn.1673-4157.2015.05.002]
 Liang Qijun,Yu Shouyi,Li Shuhua,et al.Association of thyroid nodule and metabolic syndrome[J].International Journal of Endocrinology and Metabolism,2015,(01):293.[doi:DOI:10.3760/cma.j.issn.1673-4157.2015.05.002]
[6]王锐 陆强 王术艺 朱东林 蒋韬 马宁 刘悦 李燕莉 贾晓娇 姚立新.血清25羟维生素D及vaspin水平与妊娠糖尿病关系的研究[J].国际内分泌代谢杂志,2015,(05):306.[doi:DOI:10.3760/cma.j.issn.1673-4157.2015.05.005]
 Wang Rui,Lu Qiang,Wang Shuyi,et al.Relationship between serum 25-hydroxyvitamin D, vaspin and gestational diabetes mellitus[J].International Journal of Endocrinology and Metabolism,2015,(01):306.[doi:DOI:10.3760/cma.j.issn.1673-4157.2015.05.005]
[7]黄祺 程海燕 卜瑞芳.1-磷酸鞘胺醇与胰岛素抵抗[J].国际内分泌代谢杂志,2015,(05):348.[doi:DOI:10.3760/cma.j.issn.1673-4157.2015.05.015]
 Huang Qi,Cheng Haiyan,Bu Ruifang..Sphingosine-1-phosphate and insulin resistance[J].International Journal of Endocrinology and Metabolism,2015,(01):348.[doi:DOI:10.3760/cma.j.issn.1673-4157.2015.05.015]
[8]李少卿 王坚.2型糖尿病患者血25-羟维生素D3缺乏 对胰岛素抵抗及骨量的影响[J].国际内分泌代谢杂志,2015,(06):384.[doi:DOI:10.3760/cma.j.issn.1673-4157.2015.06.006]
 Li Shaoqing*,Wang Jian..The influence of 25-hydroxyl vitamin D3 deficiency on insulin resistance and bone mass in patients with type 2 diabetes[J].International Journal of Endocrinology and Metabolism,2015,(01):384.[doi:DOI:10.3760/cma.j.issn.1673-4157.2015.06.006]
[9]张琳,费雯婕,宋光耀.网膜素与代谢综合征[J].国际内分泌代谢杂志,2016,36(01):42.[doi:10.3760/cma.j.issn.1673-4157.2016.01.010]
 Zhang Lin*,Fei Wenjie,Song Guangyao..Omentin and metabolic syndrome[J].International Journal of Endocrinology and Metabolism,2016,36(01):42.[doi:10.3760/cma.j.issn.1673-4157.2016.01.010]
[10]汤玮娜,任寿安.间歇低氧对大鼠胰岛素抵抗及骨骼肌细胞 GLUT4、Akt2的影响[J].国际内分泌代谢杂志,2016,36(02):112.[doi:10.3760/cma.j.issn.1673-4157.2016.02.009]
 Tang Weina*,Ren Shou'an..Effects of intermittent hypoxia on insulin resistance and expression of GLUT4 and Akt2 in skeletal muscle cells of rats[J].International Journal of Endocrinology and Metabolism,2016,36(01):112.[doi:10.3760/cma.j.issn.1673-4157.2016.02.009]

更新日期/Last Update: 2016-01-20