[1]刘丹丹,王丽,史兴晔,等.脂肪酸受体GPR120影响胰岛素受体底物-1的表达[J].国际内分泌代谢杂志,2014,(06):371-374.[doi:10.3760/cma.j.issn.1673-4157.2014.06.003]
　Liu Dandan*,Wang Li,Shi Xingye,et al.Regulation of fatty acid receptor GPR120 on the expression of insulin receptor substrate-1[J].International Journal of Endocrinology and Metabolism,2014,(06):371-374.[doi:10.3760/cma.j.issn.1673-4157.2014.06.003]
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脂肪酸受体GPR120影响胰岛素受体底物-1的表达()
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《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
期数:
2014年06期
页码:
371-374
栏目:
论著
出版日期:
2014-12-20

文章信息/Info

Title:
Regulation of fatty acid receptor GPR120 on the expression of insulin receptor substrate-1
作者:
刘丹丹; 王丽; 史兴晔; 张慧娟; 姜晓艳
150001 哈尔滨医科大学附属第一医院内分泌科(刘丹丹,史兴晔,张慧娟,姜晓艳); 150001 哈尔滨,中国农业科学院哈尔滨兽医研究所(王丽)
Author(s):
Liu Dandan*;  Wang Li;  Shi Xingye;  Zhang Huijuan; Jiang Xiaoyan.
*Department of Endocrinology, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China
关键词:
游离脂肪酸;  胰岛素受体底物-1;  G蛋白耦联受体120;  胰岛素抵抗
Keywords:
Free fatty acids;  Insulin receptor substrate-1;  G protein-coupled receptor 120;  Insulin resistance
DOI:
10.3760/cma.j.issn.1673-4157.2014.06.003
摘要:
目的 研究在3T3-L1脂肪细胞中G蛋白耦联受体120(GPR120)与胰岛素受体底物-1(IRS-1)的关系。方法 利用经典“鸡尾酒法”诱导3T3-L1前脂肪细胞分化,以未诱导组作为阴性对照,通过油红O染色法检测细胞内脂滴的形成情况,从而确定前脂肪细胞分化为脂肪细胞,再利用实时PCR方法检测GPR120 mRNA的表达水平。采用siRNA技术下调3T3-L1前脂肪细胞中GPR120的表达,以无关干扰组为阴性对照,干扰24 h后诱导细胞分化,诱导后于3T3-L1脂肪细胞培养液中加入软脂酸孵育24 h,分别用实时 PCR和Western印迹方法检测3T3-L1脂肪细胞中IRS-1的表达水平。结果 诱导分化的3T3-L1脂肪细胞中,GPR120 mRNA表达量较未诱导组明显升高(P<0.05); 干扰GPR120表达后3T3-L1脂肪细胞中脂滴体积和数量明显减小。另外,GPR120表达的量降低后,IRS-1 mRNA和蛋白表达水平均显著降低(P<0.05)。结论 GPR120参与胰岛素信号通路中IRS-1的表达调控。
Abstract:
Objective To investigate the correlation between G protein-coupled receptor 120(GPR120)and insulin receptor substrate-1(IRS-1)in 3T3-L1 cells. Methods A classic "cocktail" method was applied for induction of preadipocyte differentiation, and cells without induction were selected as a negative control. To determine the differentiation of preadipocyte to adipocyte, oil red O method was used to test the content of lipid droplet in cells. Real-time PCR was used to assess the expression of GRP120 mRNA.A specific siRNA was used to intervene the expression of GRP120 in 3T3-L1 preadipocyte, and no interfering group was used as a negative control. After interfering for 24 hours, 3T3-L1 preadipocyte was incubated with palmitic acid for another 24 hours to induce the differentiation. Real-time PCR and Western blot were used to detect the expression of IRS-1 mRNA and protein. Results The mRNA level of GPR120 was up-regulated in differentiated 3T3-L1 preadipocyte compared with control group(P<0.05).The volume and quantity of lipid droplet in 3T3-L1 adipocyte were significantly decreased after interfering the expression of GPR120 expression. Furthermore, IRS-1 mRNA and protein levels were significantly reduced(P<0.05).Conclusion GPR120 plays a regulatory role in the expression of IRS-1 in insulin signaling pathway.

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备注/Memo

备注/Memo:
基金项目:黑龙江省教育厅科学技术研究项目(12511240)
更新日期/Last Update: 2014-12-20