[1]白丹丹,任寿安.间歇低氧大鼠肝细胞GLUT-2、GCK表达 与胰岛素抵抗的相关性研究[J].国际内分泌代谢杂志,2017,37(04):217-220.
　Bai Dandan*,Ren Shou'an..Association between the expression of GLUT-2, GCK in hepatocytes and insulin resistance in rats treated by intermittent hypoxia[J].International Journal of Endocrinology and Metabolism,2017,37(04):217-220.
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间歇低氧大鼠肝细胞GLUT-2、GCK表达 与胰岛素抵抗的相关性研究()
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《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
37
期数:
2017年04期
页码:
217-220
栏目:
论著
出版日期:
2017-07-20

文章信息/Info

Title:
Association between the expression of GLUT-2, GCK in hepatocytes and insulin resistance in rats treated by intermittent hypoxia
作者:
白丹丹; 任寿安
030001 太原,山西医科大学(白丹丹); 030001 太原,山西医科大学第一医院呼吸科(任寿安)
Author(s):
Bai Dandan*;  Ren Shou'an.
*Shanxi Medical University, Taiyuan 030001, China
关键词:
间歇低氧;  胰岛素抵抗;  葡萄糖转运蛋白-2;  葡萄糖激酶
Keywords:
Intermittent hypoxia;  Insulin resistance;  Glucose transporter-2;  Glucokinase
文献标志码:
A
摘要:
目的 检测葡萄糖转运蛋白-2(GLUT-2)、葡萄糖激酶(GCK)在间歇低氧大鼠模型肝细胞中表达的变化,探讨间歇低氧引起胰岛素抵抗的相关机制。方法 24只6周龄健康雄性Sprague-Dawley(SD)大鼠按照随机数字表法分为对照组、间歇低氧4周组(IH4组)和间歇低氧8周组(IH8组),每组8只。间歇低氧组按预设通气模式每天给予间歇低氧暴露8 h,对照组给予间歇压缩空气,暴露时间同IH4组。实验结束后测定各组大鼠空腹血糖、空腹胰岛素,计算稳态模型评估-胰岛素抵抗指数(HOMA-IR)及胰岛素敏感性指数(ISI)。免疫组织化学染色观察肝细胞GLUT-2、GCK蛋白表达变化,并利用平均灰度值对蛋白进行定量分析。结果 与对照组相比,IH4组及IH8组空腹血糖、空腹胰岛素、HOMA-IR均升高,ISI均降低,且IH8组更明显(F=161.92、51.46、126.99、83.87,P均<0.05)。与对照组相比,IH4组及IH8组肝细胞GLUT-2、GCK蛋白表达均降低,且IH8组更显著(F=184.91、240.85,P均<0.05)。Pearson相关分析显示,GLUT-2、GCK平均灰度值与ISI呈负相关(r=-0.886、-0.906,P均<0.05),与HOMA-IR呈正相关(r=0.894、0.869,P均<0.05)。结论 间歇低氧暴露使大鼠肝细胞GLUT-2、GCK蛋白表达下调,可能参与间歇低氧条件下胰岛素抵抗的发生。
Abstract:
Objective To determine the expression of glucose transporter-2(GLUT-2)and glucokinase(GCK)in hepatocytes of intermittent hypoxic rat model, and explore the possible mechanisms of insulin resistance caused by intermittent hypoxia.Methods Twenty-four healthy male Sprague-Dawley(SD)rats at 6-week age were divided into control group(NC group),4 weeks of intermittent hypoxia group(IH4 group)and 8 weeks of intermittent hypoxia group(IH8 group)according to the random number table, with 8 rats in each group. According to the preset ventilation mode, rats in intermittent groups were exposed in intermittent hypoxia for 8 hours every day, whereas rats in NC group were exposed in intermittent compressed air for the same time with IH4 group. Fasting blood glucose and fasting insulin were tested after experiment; homeostasis model assessment-insulin resistance index(HOMA-IR)and insulin sensitivity index(ISI)were calculated. The expression of hepatic GLUT-2 and GCK protein were measured by immunohistochemical method, and were quantitatively analyzed by the average gray value.Results Compared with NC group, fasting blood glucose,fasting insulin and HOMA-IR were increased, whereas ISI were decreased in IH4 group and IH8 group, especially in IH8 group(F=161.92, 51.46, 126.99, 83.87, all P<0.05). Compared with NC group,the expression of GLUT-2 and GCK in hepatic cells were decreased in IH4 group and IH8 group, especially in IH8 group(F=184.91, 240.85, all P<0.05). Pearson correlation analysis showed that the level of GLUT-2 and GCK were negatively correlated with ISI(r=-0.886, -0.906, all P<0.05), and positively correlated with HOMA-IR(r=0.894, 0.869, all P<0.05).Conclusion Intermittent hypoxia decreases the expression of GLUT-2 and GCK, which may participate in the occurrence of insulin resistance under the condition of intermittent hypoxia.

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备注/Memo

备注/Memo:
基金项目:山西省自然科学基金资助项目(2013011048-4)
通信作者:任寿安,Email:renshouan@163.com
更新日期/Last Update: 2017-07-30