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　Chang Haiyao,Liu Jing,Wu Wenhao,et al.Relationship between angiogenin-like protein 6, leptin and diabetic nephropathy[J].International Journal of Endocrinology and Metabolism,2020,40(05):289-293.[doi:10.3760/cma.j.cn121383-20200208-02009]
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血管生成素样蛋白6、瘦素与糖尿病肾病的关系()
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《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
40
期数:
2020年05期
页码:
289-293
栏目:
论著
出版日期:
2020-09-20

文章信息/Info

Title:
Relationship between angiogenin-like protein 6, leptin and diabetic nephropathy
作者:
常海瑶1; 刘晶2; 吴文昊2; 牛乐乐2; 李兴2
1山西医科大学,太原 030001; 2山西医科大学第二医院内分泌科,太原 030009
Author(s):
Chang Haiyao1;  Liu Jing2;  Wu Wenhao2;  Niu Lele2;  Li Xing2
1Shanxi Medical University, Taiyuan 030001, China; 2 Department of Endocrinology, The Second Hospital of Shanxi Medical University, Taiyuan 030009, China Corresponding author: Li Xing, Email:13503504180@163.com
关键词:
2型糖尿病;  糖尿病肾病;  血管生成素样蛋白6;  瘦素
Keywords:
Type 2 diabetes mellitus;  Diabetic nephropathy;  Angiogenin-like protein 6;  Leptin
DOI:
10.3760/cma.j.cn121383-20200208-02009
文献标志码:
A
摘要:
目的 测定糖尿病肾病(DN)患者血清血管生成素样蛋白6(ANGPTL6)及瘦素水平,探讨ANGPTL6、瘦素与DN的关系。方法 选取2018年12月—2019年12月于山西医科大学第二医院内分泌科住院治疗的97例2型糖尿病患者作为研究对象,按尿白蛋白排泄率(UAER)将其分为DN组(47例,UAER≥30 mg/24 h)、单纯2型糖尿病组(T2DM组,50例,UAER<30 mg/24 h),另选取58名同时间段正常体检人员作为对照组。采用酶联免疫吸附法(ELISA)检测血清ANGPTL6、瘦素水平,全自动分析仪测定空腹血糖、甘油三酯、总胆固醇、高密度脂蛋白-胆固醇、低密度脂蛋白-胆固醇; 放射免疫法测定空腹胰岛素,采用稳态模型评估胰岛素抵抗指数,Pearson相关分析二者与生化指标的相关性,多元线性回归分析影响UAER的因素。结果 与对照组、T2DM组相比,DN组ANGPTL6水平显著升高(F=13.53,P<0.05),ANGPTL6与年龄、收缩压、稳态模型评估-胰岛素抵抗指数(HOMA-IR)、HbA1c、UAER、瘦素呈正相关(r=0.23、0.30、0.17、0.21、0.23、0.29,P均<0.05); 瘦素在对照组、T2DM组、DN组呈逐渐升高趋势(F=6.09,P<0.05),瘦素与年龄、体重指数、收缩压、HOMA-IR、HbA1c、UAER、ANGPTL6呈正相关(r=0.31、0.23、0.25、0.29、0.21、0.25、0.29,P均<0.05); 多元线性回归分析显示,空腹血糖(β=76.24,P<0.001)、HbA1c(β=-28.36,P<0.001)、HOMA-IR(β=-145.13,P<0.001)、ANGTPL6(β=66.15,P<0.001)、瘦素(β=8.76, P=0.02)是DN的重要影响因素。结论 DN患者ANGPTL6、瘦素水平升高,二者与DN的发生、发展密切相关。
Abstract:
Objective To measure serum levels of angiogenin-like protein 6(ANGPTL6)and leptin in patients with diabetic nephropathy(DN), and to investigate the relationship between ANGPTL6, leptin and DN.Methods A total of 97 hospitalized patients with type 2 diabetes were selected from December 2018 to December 2019 in the Department of Endocrinology, The Second Hospital of Shanxi Medical University as the research subjects.They were divided into DN group [47 cases, urinary albumin excretion rate(UAER)≥30 mg/24 h)], and single type 2 diabetes group(T2DM group, 50 cases, UAER<30 mg/24 h)according to UAER, and 58 with normal medical personnel were selected as the control group at the same time.Serum levels of ANGPTL6 and leptin were detected by enzymatic immunosorbent assay(ELISA); fasting blood glucose, triglyceride, total cholesterol, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol were measured by automatic analyzer; fasting insulin was measured by radioimmunoassay; insulin resistance index was evaluated by homeostasis model. The correlation between serum levels of ANGPTL6 and leptin was analyzed by Pearson correlation analysis, and the factors affecting UAER were analyzed by multiple linear regression.Results Compared with control group and T2DM group, ANGPTL6 level in DN group was significantly increased(F=13.53, P<0.05), and ANGPTL6 was positively correlated with age, systolic blood pressure(SBP), homeostasis model evaluation-insulin resistance index(HOMA-IR), glycated hemoglobin(HbA1c), urinary albumin excretion rate(UAER), and leptin(r=0.23, 0.30, 0.17, 0.21, 0.23, 0.29, all P<0.05). Leptin level was gradually increased from control group, T2DM group to DN group(F=6.09, P<0.05). Leptin was positively correlated with age, body mass index, SBP, HOMA-IR, HbA1c, UAER, and ANGPTL6(r=0.31, 0.23, 0.25, 0.29, 0.21, 0.25, 0.29, all P<0.05). In the multivariate linear regression analysis, fasting blood glucose(β=76.24, P<0.001), HbA1c(β=-28.36, P<0.001), HOMA-IR(β=-145.13, P<0.001), ANGTPL6(β=66.15, P<0.001), and leptin(β=8.76, P=0.02)were important factors affecting DN.Conclusion The levels of ANGPTL6 and leptin are increased in patients with DN, which are closely related to the occurrence and development of DN.

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通信作者:李兴,Email:13503504180@163.com
更新日期/Last Update: 2020-09-20