[1]孙树凯 翟玉娥 田清武 赵鹏 申井利 翟木绪.雌激素受体基因多态性与妊娠糖尿病的 相关性研究[J].国际内分泌代谢杂志,2018,38(06):361-365.[doi:10.3760/cma.j.issn.1673-4157.2018.06.001]
　Sun Shukai*,Zhai Yu'e,Tian Qingwu,et al.Correlation between polymorphism of estrogen receptor gene and gestational diabetes mellitus[J].International Journal of Endocrinology and Metabolism,2018,38(06):361-365.[doi:10.3760/cma.j.issn.1673-4157.2018.06.001]
点击复制

雌激素受体基因多态性与妊娠糖尿病的 相关性研究()
分享到：

《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
38
期数:
2018年06期
页码:
361-365
栏目:
论著
出版日期:
2018-11-20

文章信息/Info

Title:
Correlation between polymorphism of estrogen receptor gene and gestational diabetes mellitus
作者:
孙树凯 翟玉娥 田清武 赵鹏 申井利 翟木绪
作者单位:266555 青岛大学附属医院检验科(孙树凯、翟玉娥、田清武、赵鹏、申井利); 255000 淄博市妇幼保健院内分泌科(翟木绪)
Author(s):
Sun Shukai*;  Zhai Yu'e;  Tian Qingwu;  Zhao Peng;  Shen Jingli;  Zhai Muxu
*Department of Labaoratory, The Affiliated Hospital of Qingdao University, Qingdao 266555, China
关键词:
雌激素受体;  基因多态性;  单核苷酸多态性;  妊娠糖尿病
Keywords:
Estrogen receptor;  Gene polymorphism;  Single nucleotide polymorphism;  Gestational diabetes mellitus
DOI:
10.3760/cma.j.issn.1673-4157.2018.06.001
摘要:
目的 观察雌激素受体(ER)α和β基因多态性与妊娠糖尿病(GDM)的关系。方法 392例GDM患者(GDM组)和308名糖耐量正常孕妇(对照组)均来自2016年5月至2017年12月在淄博市妇幼保健院糖尿病筛查门诊就诊的孕妇,GDM诊断依据参照2014年中华医学会妇产科学分会产科学组与中华医学会围产医学分会妊娠合并糖尿病协作组制订的诊断标准,采用PCR-限制性片段长度多态性(RFLP)方法对ERα和β基因(XbaⅠ、PvuⅡ、RsaⅠ、AluⅠ酶切位点)多态性进行检测,并采用SHEsis在线计算平台进行单体型分析。结果 两组间ERα XbaⅠ和PvuⅡ酶切位点以及ERβ RsaⅠ酶切位点的基因型及等位基因分布频率差异均无统计学意义(P=0.870、0.287、0.904、0.982、0.725、0.720); 而ERβ AluⅠ酶切位点的基因型及等位基因分布频率差异存在统计学意义(P=0.007、0.003),携带有a等位基因的个体发生GDM的风险是A的1.559倍(95%CI:1.161～2.095,P=0.003)。GDM组R-a单体型所占频率显著高于对照组(OR=1.562,95%CI:1.162～2.099,P=0.003)。结论 GDM的遗传易感性与ERα基因多态性无关,而与ERβ基因多态性存在关联性,R、a等位基因可能是其易感等位基因。
Abstract:
Objective To investigate the relationship between genetic polymorphisms of estrogen receptor(ER)α, β and gestational diabetes mellitus(GDM).Methods A total of 392 patients with GDM(GDM group)and 308 pregnant women with normal glucose tolerance(control group)who underwent diabetes screening were included in this study in the clinic of the Maternal and Child Health Care Hospital of Zibo from May 2016 to December 2017. The diagnosis of GDM was based on the 2014 diagnostic criteria of the Department of Obstetrics and Gynecology and the Department of Perinatal Medicine of the Chinese Medical Association. Polymorphisms of ERα and ERβ genes(XbaⅠ, Pvu Ⅱ, Rsa Ⅰ, Alu Ⅰ)were examined by polymerase chain reaction-based restriction fragment length polymorphism(RFLP)and the haplotype analysis was conducted by SHEsis on-line computing platform.Results There was no significant difference in the distribution frequency of genotype and allele in enzyme sites of XbaⅠ, Pvu Ⅱ and Rsa Ⅰ between these two groups(P=0.870, 0.287, 0.904, 0.982, 0.725, 0.720). In enzyme sites of AluⅠ, there was significant difference in the distribution frequency of genotype and allele between these two groups(P=0.007, 0.003). The risk of GDM in persons with a allele was 1.559-fold compared with persons with A allele(95%CI:1.161-2.095, P=0.003).The frequency of R-a haplotype was significantly higher in GDM group than that in control group(OR=1.562,95%CI:1.162-2.099, P=0.003).Conclusion The polymorphism of ERβ gene but not ERα gene is associated with the susceptibility of GDM, and the R,a allele may be the susceptible alleles.

参考文献/References:


[1] Nikolic D,Al-Rasadi K,Al Busaidi N,et al.Incretins,pregnancy, and gestational diabetes[J].Curr Pharm Biotechnol,2016,17(7):597-602.
[2] Somiński B,Mys'liwska J,Ryba-Stanis awowska M,et al. Estrogen receptor α gene polymorphism and vascular complications in girls with type 1 diabetes mellitus[J].Mol Cell Biochem,2018,437(1-2):153-161.DOI:10.1007/s11010-017-3103-0.
[3] Motawi TM,El-Rehany MA,Rizk SM,et al.Genetic polymorphism of estrogen receptor alpha gene in Egyptian women with type Ⅱ diabetes mellitus[J].Meta Gene,2015,6:36-41.DOI:10.1016/j.mgene.2015.08.001.
[4] Mohammadi F,Pourahmadi M,Mosalanejad M,et al.Association of estrogen receptor α genes PvuⅡ and XbaⅠ polymorphisms with type 2 diabetes mellitus in the inpatient population of a hospital in southern Iran[J].Diabetes Metab J,2013,37(4):270-277.DOI:10.4093/dmj.2013.37.4.270.
[5] 中华医学会妇产科学分会产科学组,中华医学会围产医学分会妊娠合并糖尿病协作组.妊娠合并糖尿病诊治指南(2014)[J]. 中华妇产科杂志,2014,49(8):561-569. DOI:10.3760/cma.j.issn.0529-567x.2014.08.001.
[6] Jiwani A,Marseille E,Lohse N,et al.Gestational diabetes mellitus: results from a survey of country prevalence and practices[J].J Matern Fetal Neonatal Med,2012,25(6):600-610.DOI:10.3109/14767058.2011.587921.
[7] Sacks DA,Hadden DR,Maresh M,et al.Frequency of gestational diabetes mellitus at collaborating centers based on IADPSG consensus panel-recommended criteria:the Hyperglycemia and Adverse Pregnancy Outcome(HAPO)Study[J].Diabetes Care,2012,35(3):526-528.DOI:10.2337/dc11-1641.
[8] Wu L,Han L,Zhan Y,et al.Prevalence of gestational diabetes mellitus and associated risk factors in pregnant Chinese women:a cross-sectional study in Huangdao, Qingdao, China[J].Asia Pac J Clin Nutr,2018,27(2):383-388.DOI:10.6133/apjcn.032017.03.
[9] Zhu L,Shi J,Luu TN,et al.Hepatocyte estrogen receptor alpha mediates estrogen action to promote reverse cholesterol transport during Western-type diet feeding[J].Mol Metab,2018,8:106-116.DOI:10.1016/j.molmet.2017.12.012.
[10] Zhou Z,Ribas V,Rajbhandari P,et al.Estrogen receptor α protects pancreatic β-cells from apoptosis by preserving mitochondrial function and suppressing endoplasmic reticulum stress[J].J Biol Chem,2018,293(13):4735-4751.DOI:10.1074/jbc.M117.805069.
[11] Motawi TM,El-Rehany MA,Rizk SM,et al.Genetic polymorphism of estrogen receptor alpha gene in Egyptian women with type Ⅱ diabetes mellitus[J].Meta Gene,2015,6:36-41.DOI:10.1016/j.mgene.2015.08.001.
[12] Meshkani R,Saberi H,MohammadTaghvaei N,et al.Estrogen receptor alpha gene polymorphisms are associated with type 2 diabetes and fasting glucose in male subjects[J].Mol Cell Biochem,2012,359(1-2):225-233.DOI:10.1007/s11010-011-1017-9.
[13] 张力,刘淑芸.雌激素受体基因多态性与妇产科疾病关系研究进展[J].国外医学(妇产科学分册), 2006,33(3):194-197.DOI:10.3969/j.issn.1674-1870.2006.03.015.

相似文献/References:

[1]冯娟,夏维波.维生素D结合蛋白研究进展[J].国际内分泌代谢杂志,2016,36(03):209.[doi:10.3760/cma.j.issn.1673-4157.2016.03.17]
　Feng Juan,Xia Weibo.Research progress of vitamin D binding protein[J].International Journal of Endocrinology and Metabolism,2016,36(06):209.[doi:10.3760/cma.j.issn.1673-4157.2016.03.17]
[2]李兴佳,徐书杭,刘超.雌激素及其受体与甲状腺癌的关系[J].国际内分泌代谢杂志,2016,36(04):273.[doi:10.3760/cma.j.issn.1673-4157.2016.04.15]
　Li Xingjia,Xu Shuhang,Liu Chao.Relationship between estrogen, its receptors and thyroid cancer[J].International Journal of Endocrinology and Metabolism,2016,36(06):273.[doi:10.3760/cma.j.issn.1673-4157.2016.04.15]
[3]耿争光,王琴,邓凯,等.遵义人群载脂蛋白A5 c.553G/T基因多态性 与混合型高脂血症的相关性研究[J].国际内分泌代谢杂志,2017,37(01):6.[doi:10.3760/cma.j.issn.1673-4157.2017.01.02]
　Geng Zhengguang*,Wang Qin,Deng Kai,et al.Association of apolipoprotein A5 c.553G/T gene polymorphism with combined hyperlipidemia in Zunyi population[J].International Journal of Endocrinology and Metabolism,2017,37(06):6.[doi:10.3760/cma.j.issn.1673-4157.2017.01.02]
[4]刘晓妮 王颖 李桃桃 马小敏 梁彦.血管紧张素转换酶基因rs4353多态性 与糖尿病微血管病变的相关性研究[J].国际内分泌代谢杂志,2018,38(03):149.[doi:10.3760/cma.j.issn.1673-4157.2018.03.002]
　Liu Xiaoni*,Wang Ying,Li Taotao,et al.Association between angiotensin converting enzyme gene rs4353 polymorphism and diabetic microangiopathy[J].International Journal of Endocrinology and Metabolism,2018,38(06):149.[doi:10.3760/cma.j.issn.1673-4157.2018.03.002]
[5]潘娟 陈霞.2型糖尿病患者FNDC5基因多态性 与颈动脉粥样硬化相关性研究[J].国际内分泌代谢杂志,2019,39(03):155.[doi:10.3760/cma.j.issn.1673-4157.2019.03.003]
　Pan Juan,Chen Xia.Association between FNDC5 gene polymorphism and carotid atherosclerosis in patients with type 2 diabetes mellitus[J].International Journal of Endocrinology and Metabolism,2019,39(06):155.[doi:10.3760/cma.j.issn.1673-4157.2019.03.003]
[6]梁绮君 余寿益 胡晨鸣 李淑华 陈诗慧 杨焱 莫巧璇.乙醛脱氢酶2基因多态性与空腹血糖受损的 相关性[J].国际内分泌代谢杂志,2019,39(04):222.[doi:10.3760/cma.j.issn.1673-4157.2019.04.002]
　Liang Qijun,Yu Shouyi,Hu Chenming,et al.Association of aldehyde dehydrogenase 2 genetic polymorphism and impaired fasting glucose[J].International Journal of Endocrinology and Metabolism,2019,39(06):222.[doi:10.3760/cma.j.issn.1673-4157.2019.04.002]
[7]王英楠,成志锋.ABCG2基因多态性与痛风[J].国际内分泌代谢杂志,2020,40(05):351.[doi:10.3760/cma.j.cn121383-20200216-02026]
　Wang Yingnan,Cheng Zhifeng.ABCG2 Gene polymorphism and gout Medical University, Harbin 150001, China[J].International Journal of Endocrinology and Metabolism,2020,40(06):351.[doi:10.3760/cma.j.cn121383-20200216-02026]
[8]高虹,杨静,高飞.维生素D受体基因多态性与亚洲绝经后女性骨密度相关性的荟萃分析[J].国际内分泌代谢杂志,2021,41(03):158.[doi:10.3760/cma.j.cn121383-20210225-02058]
　Gao Hong,Yang Jing,Gao Fei..A meta-analysis of the association between vitamin D receptor gene polymorphism and bone mineral density in Asian postmenopausal women[J].International Journal of Endocrinology and Metabolism,2021,41(06):158.[doi:10.3760/cma.j.cn121383-20210225-02058]
[9]卓然然,付盼盼.IL-16基因多态性与GDM的关系及对妊娠结局的影响[J].国际内分泌代谢杂志,2022,42(02):118.[doi:10.3760/cma.j.cn121383-20210106-01014]
　Zhuo Ranran,Fu Panpan..Association between IL-16 gene polymorphism and susceptibility to GDM and its influence on pregnancy outcome[J].International Journal of Endocrinology and Metabolism,2022,42(06):118.[doi:10.3760/cma.j.cn121383-20210106-01014]
[10]李赫岩,滕卫平,单忠艳,等.内分泌腺体中磷酸二酯酶的分布及作用研究进展[J].国际内分泌代谢杂志,2022,42(02):129.[doi:10.3760/cma.j.cn121383-20201201-12003]
　Li Heyan,Teng Weiping,Shan Zhongyan,et al.Distribution and function of phosphodiesterases in endocrine glands[J].International Journal of Endocrinology and Metabolism,2022,42(06):129.[doi:10.3760/cma.j.cn121383-20201201-12003]

备注/Memo

备注/Memo:
基金项目:山东省医药卫生科技发展计划项目(2017WS493)
Fund program:Medicine and Health Science Technology Development Project of Shandong Province(2017WS493)
作者单位:266555 青岛大学附属医院检验科(孙树凯、翟玉娥、田清武、赵鹏、申井利); 255000 淄博市妇幼保健院内分泌科(翟木绪)
通信作者:翟木绪,Email:mxzhai@163.com
更新日期/Last Update: 2018-12-30