[1]杨薇,李春君,孙蓓,等.雷公藤多苷对糖尿病肾病大鼠核因子-κB 信号通路的影响[J].国际内分泌代谢杂志,2016,36(02):107-111.[doi:10.3760/cma.j.issn.1673-4157.2016.02.008]
　Yang Wei*,Li Chunjun,Sun Bei,et al.Effects of triperygium wilfordii polyglucoside on nuclear factor-κB signaling pathway in rats with diabetic nephropathy[J].International Journal of Endocrinology and Metabolism,2016,36(02):107-111.[doi:10.3760/cma.j.issn.1673-4157.2016.02.008]
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雷公藤多苷对糖尿病肾病大鼠核因子-κB 信号通路的影响()
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《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
36
期数:
2016年02期
页码:
107-111
栏目:
论著
出版日期:
2016-03-20

文章信息/Info

Title:
Effects of triperygium wilfordii polyglucoside on nuclear factor-κB signaling pathway in rats with diabetic nephropathy
作者:
杨薇; 李春君; 孙蓓; 厉莉; 王珊珊; 郭欣; 张晓娜; 马泽军; 陈莉明
300070 天津医科大学(杨薇); 300070 天津医科大学代谢病医院糖尿病肾病科,卫生部激素与发 育重点实验室(李春君,孙蓓,厉莉,王珊珊,郭欣,张晓娜,马泽军,陈莉明)
Author(s):
Yang Wei*;  Li Chunjun;  Sun Bei;  Li Li;  Wang Shanshan;  Guo Xin;  Zhang Xiaona;  Ma Zejun;  Chen Liming.
* Tianjin Medical University,Tianjin 300070, China
关键词:
雷公藤多苷;  糖尿病肾病;  核因子-κB;  细胞间黏附分子-1;  白细胞介素-6
Keywords:
Triperygium wilfordii polyglucoside;  Diabetic nephropathy;  Nuclear factor-κB;  Intercellular adhesion molecule-1;  Interleukin-6
DOI:
10.3760/cma.j.issn.1673-4157.2016.02.008
摘要:
目的 探讨不同剂量雷公藤多苷(TWP)对糖尿病肾病(DN)大鼠肾组织核因子-κB信号通路的影响。方法 选取60只Sprague-Dawley(SD)大鼠,给予高糖、高脂饮食联合尾静脉注射链脲佐菌素(30 mg/kg)建立DN大鼠模型。将成模大鼠按照随机数字表法分为DN对照组(DNC组,n=8)、低剂量TWP治疗组(3 mg·kg-1·d-1,n=8)、中剂量TWP治疗组(6 mg·kg-1·d-1,n=8)、高剂量TWP治疗组(9 mg·kg-1·d-1,n=10)。选取10只大鼠作为正常对照组(NC组),喂养常规饲料,灌胃8周后检测大鼠的体重、肾重体重比、尿微量白蛋白、血糖、肝功能、肾功能,并采用HE染色观察肾脏形态改变,分别采用免疫组化法、q-PCR法、Western印迹方法对大鼠肾脏核因子-κB、细胞间黏附分子-1(ICAM-1)以及白细胞介素-6(IL-6)进行定位定量分析(以观察不同剂量TWP对大鼠肾脏核因子-κB、ICAM-1、IL-6表达的影响)。结果 TWP治疗可减少尿白蛋白,但对体重、血糖、肝功能、肾功能无影响,可降低DN大鼠肾脏核因子-κB、ICAM-1、IL-6 mRNA(t核因子-κB=8.89～16.88,tICAM-1=9.56～15.92,tIL-6=10.16～25.78,P均<0.05及蛋白(t核因子-κB=9.87～17.38,tICAM-1=8.54～16.95,tIL-6=9.76～20.18,P均<0.05)表达,且呈剂量依赖性,中高剂量TWP抑制更明显(P均<0.05)。结论 TWP呈剂量依懒性抑制DN大鼠肾组织核因子-κB炎性反应信号通路。
Abstract:
Objective To investigate the effects of different doses of triperygium wilfordii polyglucoside(TWP)on the nuclear factor-κB signaling pathway in rats with diabetic nephropathy(DN).Methods A total of 60 Sprague-Dawley(SD)rats were seleted. DN rats model were established by high-glucose and high fat diet, in combination with streptozotocin injection from tail vein. DN rats were divided into diabetic normal control group(DNC group, n=8), low dose of TWP group(L-TWP group,3 mg·kg-1·d-1, n=8)group, median dose of TWP group(M-TWP group,6 mg·kg-1·d-1, n=8), and high dose of TWP group(H-TWP group,9 mg·kg-1·d-1, n=10)according to the random number table method. Ten healthy rats were used as control group. After 8 weeks of intervention, body weight(BW), kidney weight/body weight(KW/BW), urine microalbumin(UMA), blood glucose, liver function and renal function were examined. HE staining was used to observe the changes of kidney morphology. Immunohistochemical method, q-PCR and Western blotting were used to analyze the location and expression level of nuclear factor-κB, intercellular adhesion molecule-1(ICAM-1)and interleukin-6(IL-6). The effects of different doses of TWP on the expression of nuclear factor-κB, ICAM-1 and IL-6 in rats with DN. Results TWP therapy decreased UMA but did not affect BW, KW/BW, liver function and renal function. TWP also down-regulated the expression of nuclear factor-κB,ICAM-1,IL-6 mRNA(tNuclear factor-κB=8.89-16.88, tICAM-1=9.56-11.67, tIL-6=10.16-25.78, all P<0.05)as well as the protein level(tNuclear factor-κB=9.87-17.38,tICAM-1=8.54-16.95,tIL-6=9.76-20.18, all P<0.05)in dose-dependent manner; the effects were robust in L-TWP and H-TWP group(all P<0.05). Conclusion TWP suppresses inflammatory signaling pathways of nuclear factor-κB in a dose-dependent manner.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81273915); 天津市自然科学基金资助项目(14JCYBJC26200)
更新日期/Last Update: 2016-03-20