[1]王海敏,汤旭磊.白藜芦醇对去卵巢骨质疏松大鼠血同型 半胱氨酸和氧化应激指标的影响[J].国际内分泌代谢杂志,2016,36(01):20-23.[doi:10.3760/cma.j.issn.1673-4157.2016.01.005]
　Wang Haimin,Tang Xulei..Effects of resveratrol on plasma homocysteine and oxidative stress in ovariectomized osteoporotic rats[J].International Journal of Endocrinology and Metabolism,2016,36(01):20-23.[doi:10.3760/cma.j.issn.1673-4157.2016.01.005]
点击复制

白藜芦醇对去卵巢骨质疏松大鼠血同型 半胱氨酸和氧化应激指标的影响()
分享到：

《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
36
期数:
2016年01期
页码:
20-23
栏目:
论著
出版日期:
2016-01-20

文章信息/Info

Title:
Effects of resveratrol on plasma homocysteine and oxidative stress in ovariectomized osteoporotic rats
作者:
王海敏; 汤旭磊
730000 兰州大学第一医院内分泌科(第一作者现在濮阳市安阳地区医院内分泌科)
Author(s):
Wang Haimin; Tang Xulei.
Department of Endocrinology,The First Hospital of Lanzhou University,Lanzhou 730000,China
关键词:
白藜芦醇;  骨质疏松;  同型半胱氨酸;  氧化应激
Keywords:
Resveratrol;  Osteoporosis;  Homocysteine;  Oxidative stress
DOI:
10.3760/cma.j.issn.1673-4157.2016.01.005
摘要:
目的 观察白藜芦醇对去卵巢骨质疏松大鼠血同型半胱氨酸(Hcy)和氧化应激指标的影响。方法 32只3月龄雌性Sprague-Dawley(SD)大鼠,按随机数字表法分为4组:去卵巢组、乙烯雌酚组、白藜芦醇组和假手术组,每组8只。去卵巢组、乙烯雌酚组和白藜芦醇组大鼠行双侧卵巢切除术复制骨质疏松模型。术后1周开始灌胃给药,假手术组和去卵巢组大鼠给予蒸馏水(每日一次),其余两组大鼠分别予乙烯雌酚0.03 mg·kg-1·d-1和白藜芦醇20 mg·kg-1·d-1。治疗10周后,测定大鼠血浆总Hcy(tHcy)、总抗氧化能力(TAC)、谷胱甘肽过氧化物酶(GSH-Px)和丙二醛水平,取L5椎体和右股骨进行骨密度测定。结果 与假手术组相比,去卵巢组血浆tHcy和丙二醛水平升高,TAC和 GSH-Px水平下降; 与去卵巢组相比,乙烯雌酚组和白藜芦醇组血浆tHcy和丙二醛浓度均明显降低,TAC和GSH-Px水平显著升高(F=19.709,117.219,7.020,20.544,P均<0.01); 白藜芦醇组血浆丙二醛浓度较乙烯雌酚组升高(F=117.219,P<0.01)。与假手术组相比,去卵巢组腰椎和股骨骨密度均明显降低; 与去卵巢组相比,乙烯雌酚组腰椎和股骨骨密度明显升高,接近假手术组水平(P>0.05); 白藜芦醇组腰椎和股骨骨密度升高,但仍低于乙烯雌酚组和假手术组(F=48.518,43.263,P均<0.01)。血浆tHcy与腰椎和股骨骨密度呈负相关(r=-0.663,P <0.001; r=-0.520,P=0.002), TAC与股骨骨密度呈正相关(r=0.539,P=0.001)。结论 高Hcy血症和氧化应激与去卵巢大鼠骨质疏松关系密切。白藜芦醇可能通过降低血浆tHcy水平和改善氧化应激,对去卵巢大鼠骨质疏松发挥保护作用。
Abstract:
Objective To investigate the effects of resveratrol on plasma homocysteine and oxidative stress markers in ovariectomized(OVX)osteoporotic rats.Methods Thirty-two three-month-old female Sprague-Dawley(SD)rats were randomly divided into four groups according to the random number table, which were OVX group, diethylstilbestrol group, resveratrol group and sham-operated group, with eight rats in each group. The osteoporotic models were established by bilateral ovariectomy in OVX group,diethylstilbestrol group and resveratrol group. Gastric gavage was started from one week after surgery. Rats in sham-operated group and OVX group were given distilled water once a day. Rats in the other two groups were given diethylstilbestrol(0.03 mg·kg-1·d-1 )and resveratrol( 20 mg·kg-1·d-1), respectively.After treatment for 10 weeks,levels of total homocysteine(tHcy), total antioxidant capacity(TAC),glutathione peroxidase(GSH-Px)and malondialdehyde(MDA)were measured. The 5th lumbar vertebrae and right femur were removed to determine bone mineral density(BMD). Results Compared with sham-operated group, plasma levels of tHcy and MDA were significantly increased, whereas levels of TAC and GSH-Px were remarkably decreased in OVX group. Compared with OVX group, plasma levels of tHcy and MDA were significantly decreased, but levels of TAC and GSH-Px were increased(F=19.709, 117.219,7.020,20.544, all P<0.01)in diethylstilbestrol group and resveratrol group. Plasma level of MDA in resveratrol group was higher than that in diethylstilbestrol group(F=117.219,P<0.01).Compared with sham-operated group, BMD of lumbar vertebrae and femur were decreased in OVX group.Compared with OVX group, BMD of lumbar vertebrae and femur were remarkably improved in diethylstilbestrol group, which were similar to sham-operated group(P>0.05). BMD of lumbar vertebrae and femur were remarkably increased in resveratrol group, but were still lower than those of diethylstilbestrol group and sham-operated group(F=48.518,43.263, all P<0.01). Plasma tHcy was negatively correlated with BMD of lumbar vertebrae and femur(r=-0.663,P<0.001; r=-0.520, P=0.002). A positive correlation was observed between plasma TAC and BMD of femur(r=0.539,P=0.001). Conclusions Hyperhomocysteinemia and oxidative stress are closely related to osteoporosis in OVX rats. Resveratrol may protect against osteoporosis by decreasing plasma level of tHcy and improving oxidative stress in OVX rats.

参考文献/References:

[1] Bahtiri E,Islami H,Rexhepi S,et al. Relationship of homocysteine levels with lumbar spine and femur neck BMD in postmenopausal women[J].Acta Reumatol Port,2015, [Epub ahead of print].
[2] Sharma T,Islam N,Ahmad J,et al. Correlation between bone mineral density and oxidative stress in postmenopausal women[J].Indian J Endocrinol Metab,2015,19(4):491-497.DOI:10.4103/2230-8210.159053.
[3] Chow SE,Hshu YC,Wang JS,et al. Resveratrol attenuates oxLDL-stimulated NADPH oxidase activity and protects endothelial cells from oxidativefunctional damages[J].J Appl Physiol(1985),2007,102(4):1520-1527.
[4] Schroecksnadel K,Winkler C,Wirleitner B,et al. Anti-inflammatory compound resveratrol suppresses homocysteine formation in stimulated human peripheralblood mononuclear cells in vitro[J].Clin Chem Lab Med,2005,43(10):1084-1088.
[5] Gjesdal CG,Vollset SE,Ueland PM,et al. Plasma total homocysteine level and bone mineral density: the Hordaland Homocysteine Study[J].Arch Intern Med,2006,166(1):88-94.
[6] Herrmann M,Wildemann B,Claes L,et al. Experimental hyperhomocysteinemia reduces bone quality in rats[J].Clin Chem,2007,53(8):1455-1461.
[7] Koh JM,Lee YS,Kim YS,et al. Homocysteine enhances bone resorption by stimulation of osteoclast formation and activity through increasedintracellular ROS generation[J].J Bone Miner Res,2006,21(7):1003-1011.
[8] Koz ST,Etem EO,Baydas G,et al. Effects of resveratrol on blood homocysteine level,on homocysteine induced oxidative stress,apoptosis andcognitive dysfunctions in rats[J].Brain Res,2012,1484:29-38.DOI:10.1016/j.brainres.2012.09.026.
[9] 李明,白晓春,刘俊,等.抗氧化剂对去卵巢大鼠骨密度和血清生化指标的影响[J].中华内分泌代谢杂志,2008,24(4):368-371.DOI:10.3321/j.issn:1000-6699.2008.04.006.
[10] 傅松波,汤旭磊,杨晓梅,等.白黎芦醇对糖尿病肾损害大鼠超微结构的影响[J].中国老年学杂志,2012,32(18):3965-3967.DOI:10.3969/j.issn.1005-9202.2012.18.046.

相似文献/References:

[1]吴开泽 林文锋 方淑斌 张振.阿仑膦酸钠与特立帕肽不同联合方案治疗骨质疏松[J].国际内分泌代谢杂志,2015,(05):340.[doi:DOI:10.3760/cma.j.issn.1673-4157.2015.05.013]
　Wu Kaize*,Lin Wenfeng,Fang Shubin,et al.Different combination of alendronate and teriparatide in the treatment of osteoporosis[J].International Journal of Endocrinology and Metabolism,2015,(01):340.[doi:DOI:10.3760/cma.j.issn.1673-4157.2015.05.013]
[2]刘洲君,胡咏新,刘超.肠道益生菌对骨代谢的影响[J].国际内分泌代谢杂志,2016,36(01):55.[doi:10.3760/cma.j.issn.1673-4157.2016.01.014]
　Liu Zhoujun,Hu Yongxin,Liu Chao..Effects of intestinal probiotics on bone metabolism[J].International Journal of Endocrinology and Metabolism,2016,36(01):55.[doi:10.3760/cma.j.issn.1673-4157.2016.01.014]
[3]冯娟,夏维波.维生素D结合蛋白研究进展[J].国际内分泌代谢杂志,2016,36(03):209.[doi:10.3760/cma.j.issn.1673-4157.2016.03.17]
　Feng Juan,Xia Weibo.Research progress of vitamin D binding protein[J].International Journal of Endocrinology and Metabolism,2016,36(01):209.[doi:10.3760/cma.j.issn.1673-4157.2016.03.17]
[4]孔晶,王鸥,邢小平.狄诺塞麦治疗代谢性骨病的研究进展[J].国际内分泌代谢杂志,2016,36(05):334.[doi:10.3760/cma.j.issn.1673-4157.2016.05.13]
　Kong Jing,Wang Ou,Xing Xiaoping.Update on denosumab treatment in metabolic bone diseases[J].International Journal of Endocrinology and Metabolism,2016,36(01):334.[doi:10.3760/cma.j.issn.1673-4157.2016.05.13]
[5]韩松梅,陈莉丽,李强,等.2型糖尿病患者不同HbA1c水平和骨代谢 指标的关系[J].国际内分泌代谢杂志,2017,37(05):293.
　Han Songmei*,Chen Lili,Li Qiang,et al.Relationship between different HbA1c levels and bone metabolic markers in type 2 diabetic patients[J].International Journal of Endocrinology and Metabolism,2017,37(01):293.
[6]李霞 雷涛.铁过载与代谢性疾病的关系[J].国际内分泌代谢杂志,2018,38(01):29.[doi:10.3760/cma.j.issn.1673-4157.2018.01.008]
　Li Xia*,Lei Tao..Relationship between iron overload and metabolic diseases[J].International Journal of Endocrinology and Metabolism,2018,38(01):29.[doi:10.3760/cma.j.issn.1673-4157.2018.01.008]
[7]胡咏新 郑仁东 刘洲君 刘超.2017年骨质疏松年度进展[J].国际内分泌代谢杂志,2018,38(06):423.[doi:10.3760/cma.j.issn.1673-4157.2018.06.016]
　Hu Yongxin,Zheng Rendong,Liu Zhoujun,et al.Annual development of osteoporosis in 2017[J].International Journal of Endocrinology and Metabolism,2018,38(01):423.[doi:10.3760/cma.j.issn.1673-4157.2018.06.016]
[8]王倩,刘亚平.卵泡刺激素对骨代谢作用的研究进展[J].国际内分泌代谢杂志,2023,43(04):305.[doi:10.3760/cma.j.cn121383-20220307-03016]
　Wang Qian,Liu Yaping.Research progress of follicle stimulating hormone on bone metabolism[J].International Journal of Endocrinology and Metabolism,2023,43(01):305.[doi:10.3760/cma.j.cn121383-20220307-03016]
[9]刘元君,陈国芳,刘超.不同饮食模式对骨代谢的影响:现状与展望[J].国际内分泌代谢杂志,2023,43(06):500.[doi:10.3760/cma.j.cn121383-20221009-10011]
　Liu Yuanjun,Chen Guofang,Liu Chao..Effects of different dietary patterns on bone metabolism: current status and prospects[J].International Journal of Endocrinology and Metabolism,2023,43(01):500.[doi:10.3760/cma.j.cn121383-20221009-10011]
[10]叶缪敏,夏寅,李倩.MOTS-c与代谢相关性疾病的关系及作用机理[J].国际内分泌代谢杂志,2024,44(01):27.[doi:10.3760/cma.j.cn121383-20221211-12027]
　Ye Miaomin,Xia Yin,Li Qian.Function and mechanism of MOTS-c in metabolic-related diseases[J].International Journal of Endocrinology and Metabolism,2024,44(01):27.[doi:10.3760/cma.j.cn121383-20221211-12027]

更新日期/Last Update: 2016-01-20