[1]霍琰,刘素新,张文辉,等.Visfatin在妊娠糖尿病孕妇血清及胎盘组织中的表达[J].国际内分泌代谢杂志,2014,(05):300-304.[doi:10.3760/cma.j.issn.1673-4157.2014.05.003]
 Huo Yan,Liu Suxin,Zhang Wenhui,et al.Circulating levels of visfatin and its expression in placental tissue in pregnant women with gestational diabetes[J].International Journal of Endocrinology and Metabolism,2014,(05):300-304.[doi:10.3760/cma.j.issn.1673-4157.2014.05.003]
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Visfatin在妊娠糖尿病孕妇血清及胎盘组织中的表达()
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《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
期数:
2014年05期
页码:
300-304
栏目:
论著
出版日期:
2014-10-31

文章信息/Info

Title:
Circulating levels of visfatin and its expression in placental tissue in pregnant women with gestational diabetes
作者:
霍琰刘素新张文辉张洁范艳丽靳颖冯静
050051 石家庄,河北省人民医院妇产科
Author(s):
Huo Yan Liu Suxin Zhang Wenhui Zhang Jie Fan Yanli Jin Ying Feng Jing.
Department of Obstetrics and Gynecology, the People's Hospital of Hebei Province, Shijiazhuang 050051, China
关键词:
妊娠糖尿病 内脂素 过氧化物酶体增殖物活化受体
Keywords:
Gestational diabetes mellitus Visfatin Peroxisome proliferator-activated receptor γ
DOI:
10.3760/cma.j.issn.1673-4157.2014.05.003
摘要:
目的 通过测定妊娠糖尿病(GDM)及正常妊娠妇女血清visfatin、胎盘组织visfatin及过氧化物酶体增殖物活化受体(PPAR)γ的表达,探讨visfatin、PPARγ在GDM中的作用及相互关系。方法 选取2011年10月至2012年10月在河北省人民医院妇产科行择期剖宫产终止妊娠的患者57例,其中GDM组30例、正常妊娠组27名。酶联免疫吸附法测定血清visfatin水平,实时荧光定量反转录PCR测定胎盘组织visfatin及PPARγ mRNA表达水平,免疫蛋白印迹法检测胎盘组织visfatin及PPARγ蛋白的表达水平。结果 GDM组产前血清visfatin水平明显低于正常妊娠组[(6.72±3.79)ng/ml vs.(8.87±4.08)ng/ml,t=-2.06,P=0.04]; 胎盘组织visfatin mRNA及蛋白表达水平在两组间无明显差异(P>0.05); GDM组PPARγ mRNA及蛋白表达水平低于正常妊娠组[(0.26±0.11)vs.(0.60±0.41),t=-2.77,P<0.05;(0.34±0.09)vs.(0.73±0.13),t=-7.03,P<0.05],在GDM组,胎盘组织visfatin mRNA表达与PPARγ mRNA表达水平呈正相关(r=0.67,P=0.02)。结论 Visfatin在GDM的发病中起一定作用,并与胎盘组织中PPARγ mRNA表达相关。
Abstract:
Objective To investigate the serum visfatin and placental level of visfatin, peroxisome proliferator-activated receptor(PPAR)γ in pregnant women with or without gestational diabetes mellitus(GDM), analyze their co-relationship and roles in GDM. Methods Fifty-seven pregnant women were enrolled at the department of Obstetrics and Gynecology in Hebei general hospital from October 2011 to October 2012. All pregnancies were undertaken elective C-section. Thirty of these women suffered from GDM. The remaining 27 age-matched pregnant women with normal oral glucose tolerance test were served as controls. Serum visfatin level, its mRNA expression, PPARγ mRNA expression(quantitative real-time PCR)and its protein level in placental tissue from women with GDM and normal pregnant controls were measured. Results Serum visfatin was significantly lower in women with GDM compared with those in healthy controls[(6.72±3.79)ng/ml vs.(8.87±4.08)ng/ml,t=-2.06,P=0.04].However, there were no differences in visfatin mRNA and protein expression between the two groups(P>0.05). PPARγ mRNA and protein expression were lower in GDM group compared with the healthy contol group[(0.26±0.11)vs.(0.60±0.41), t=-2.77,P<0.05;(0.34±0.09)vs.(0.73±0.13),t=-7.03,P<0.05].The visfatin mRNA expression in placenta was positively correlated with PPARγ mRNA expression in GDM group(r=0.67,P=0.02). Conclusions Visfatin may play a role in the pathogenesis of GDM, and there is an association between visfatin and PPARγ in GDM.

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更新日期/Last Update: 2014-09-20