[1]邹家琦,丁雪洁,孙鹏,等.体重指数对胰岛分离产量及胰岛功能的影响[J].国际内分泌代谢杂志,2021,41(05):445-449.[doi:10.3760/cma.j.cn121383-20201204-12015]
　Zou Jiaqi,Ding Xuejie,Sun Peng,et al.The influence of body mass index on islet isolation outcome and function[J].International Journal of Endocrinology and Metabolism,2021,41(05):445-449.[doi:10.3760/cma.j.cn121383-20201204-12015]
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体重指数对胰岛分离产量及胰岛功能的影响()
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《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
41
期数:
2021年05期
页码:
445-449
栏目:
论著
出版日期:
2021-09-30

文章信息/Info

Title:
The influence of body mass index on islet isolation outcome and function
作者:
邹家琦1; 丁雪洁1; 孙鹏1; 王树森2; 刘尧娟1
1天津市第一中心医院卫生部危重病急救医学重点实验室 300384; 2天津市第一中心医院器官移植中心 300190
Author(s):
Zou Jiaqi1;  Ding Xuejie1;  Sun Peng1;  Wang Shusen2;  Liu Yaojuan1.
1Key Laboratory for Critical Care Medicine of the Ministry of Health, Tianjin First Center Hospital, Tianjin 300384, China; 2Organ Transplant Center, Tianjin First Central Hospital, Tianjin 300190, China
关键词:
体重指数;  胰岛分离;  产量;  胰岛功能
Keywords:
Body mass index;  Islets isolation;  Yield;  Function of islet
DOI:
10.3760/cma.j.cn121383-20201204-12015
摘要:
目的 探讨不同体重指数(BMI)分组的供体胰腺胰岛产量和功能差异。方法 纳入研究的93例胰腺均来自器官捐献者,根据亚洲人肥胖等级分组标准,将供体分为3组,分别为正常组(BMI:18.50～22.99 kg/m2)、超重组(BMI: 23.00～24.99 kg/m2)和肥胖组(BMI≥25.00 kg/m2)。利用Ricordi方法消化胰岛,连续密度梯度法纯化胰岛,检测胰岛分离效率、胰岛活性,葡萄糖刺激胰岛素分泌试验(GSIS)检测胰岛素分泌能力。结果 胰岛分离产量(IEQ)在肥胖组(BMI≥25.00 kg/m2)更高(F=3.05,P<0.05)。Pearson相关性分析显示供体BMI水平与胰岛功能呈正相关性(r=0.37,P=0.01)。结论 当供体BMI在一定范围内时,供体BMI水平越高,所得胰岛产量越高,且功能更好。
Abstract:
Objective To investigate the difference between the donors body mass index(BMI)and the islet isolation yield and function of islet.Methods The 93 cases of pancreas were collected for our study. According to the obesity classification standard in Asian, the donors were divided into three groups, including the normal group(BMI: 18.50-22.99 kg/m2), the overweight group(BMI: 23.00-24.99 kg/m2)and the obese group(BMI≥25.00 kg/m2). The method from Ricordi was used for digestion, and islets were purified by continuous density gradient method. The islets were identified by staining with the Dithizone(DTZ), the islets were analyzed for cell viability, and the insulin secretion ability of islets was determined by GSIS test.Results The islets yield(IEQ)in BMI≥25.00 kg/m2 group was more than other two groups(F=3.05, P<0.05). Pearson analysis showed that the level of donor BMI was positively correlated with islet function(r=0.37, P=0.01).Conclusion The higher level of donor BMI may be associated with more islets yield and better islet function in certain range of isolation BMI.

参考文献/References:

[1] Silva AI,de Matos AN,Brons IG,et al.An overview on the development of a bio-artificial pancreas as a treatment of insulin-dependent diabetes mellitus[J].Med Res Rev,2006,26(2):181-222.DOI:10.1002/med.20047.
[2] Vantyghem MC,Chetboun M,Gmyr V,et al.Ten-year outcome of islet alone or islet after kidney transplantation in type 1 diabetes:a prospective Parallel-Arm cohort study[J].Diabetes Care,2019,42(11):2042-2049.DOI:10.2337/dc19-0401.
[3] Ravi PK,Purkait S,Agrawal U,et al.Regional variation of human pancreatic islets dimension and its impact on beta cells in Indian population[J].Islets,2019,11(6):141-151. DOI:10.1080/19382014.2019.1686323.
[4] Wang L,Gao P,Zhang M,et al.Prevalence and ethnic pattern of diabetes and prediabetes in china in 2013[J].JAMA,2017,317(24):2515-2523.DOI:10.1001/jama.2017.7596.
[5] Kin T,Murdoch TB,Shapiro AM,et al.Estimation of pancreas weight from donor variables[J].Cell Transplant,2006,15(2):181-185.DOI:10.3727/000000006783982133.
[6] Nano R,Clissi B,Melzi R,et al.Islet isolation for allotransplantation:variables associated with successful islet yield and graft function[J].Diabetologia,2005,48(5):906-912.DOI:1007/s00125-005-1725-3.
[7] Hanley SC,Paraskevas S,Rosenberg L.Donor and isolation variables predicting human islet isolation success[J].Transplantation,2008,85(7):950-955.DOI:10.1097/TP.0b013e3181683df5.
[8] Proneth A,Schnitzbauer AA,Zeman F,et al.Extended pancreas donor program-the EXPAND study rationale and study protocol[J].Transplant Res,2013,2(1):12.DOI:210.1186/2047-1440-2-12.
[9] Vinkers MT,Rahmel AO,Slot MC,et al.How to recognize a suitable pancreas donor:a Eurotransplant study of preprocurement factors[J].Transplant Proc,2008,40(5):1275-1278.DOI:10.1016/j.transproceed.2008.03.142.
[10] Verma M, Rajput M,Kishore K,et al.Asian BMI criteria are better than WHO criteria in predicting Hypertension:A cross-sectional study from rural India[J].J Family Med Prim Care, 2019,8(6):2095-2100.DOI:10.4103/jfmpc.jfmpc_257_19.
[11] Matsumoto I,Sawada T,Nakano M,et al.Improvement in islet yield from obese donors for human islet transplants[J].Transplantation,2004,8(6):880-885.DOI:10.1097/01.tp.0000134396.03440.1e.
[12] Hilling DE,Bouwman E,Terpstra OT,et al.Effects of donor-,pancreas-,and isolation-related variables on human islet isolation outcome:a systematic review[J].Cell Transplant,2014,23(8):921-928.DOI:10.3727/096368913X666412.
[13] Wang LJ,Kin T,O'Gorman D,et al.A multicenter study:north american islet donor score in donor pancreas selection for human islet isolation for transplantation[J].Cell Transplant,2016,25(8):1515-1523.DOI:10.3727/096368916X691141.
[14] Wang Y,Danielson KK,Ropski A,et al.Systematic analysis of donor and isolation factor's impact on human islet yield and size distribution[J].Cell Transplant,2013,22(12):2323-2333.DOI:10.3727/096368912X662417.
[15] Ponte GM,Pileggi A,Messinger S,et al.Toward maximizing the success rates of human islet isolation:influence of donor and isolation factors[J].Cell Transplant,2007,16(6):595-607.DOI:10.3727/000000007783465082.
[16] Brandhorst H,Brandhorst D,Hering BJ,et al.Body mass index of pancreatic donors:a decisive factor for human islet isolation[J].Exp Clin Endocrinol Diabetes,1995,103 Suppl 2:23-26.DOI:10.1055/s-0029-1211388.
[17] Charles ED,Neuschwander-Tetri BA,Pablo Frias J,et al.Pegbelfermin(BMS-986036),PEGylated FGF21,in patients with obesity and type 2 diabetes:results from a randomized phase 2 study[J].Obesity(Silver Spring),2019,27(1):41-49.DOI:10.1002/oby.22344.
[18] Linnemann AK,Baan M,Davis DB.Pancreatic β-cell proliferation in obesity[J].Adv Nutr,2014,5(3):278-288.DOI:10.3945/an.113.005488.
[19] Dollerup OL,Trammell SAJ,Hartmann B,et al.Effects of nicotinamide riboside on endocrine pancreatic function and incretin hormones in nondiabetic men with obesity[J].J Clin Endocrinol Metab,2019,104(11):5703-5714.DOI:10.1210/jc.2019-01081.
[20] Liu T,Sun P,Zou J,et al.Increased frequency of β cells with abnormal NKX6.1 expression in type 2 diabetes but not in subjects with higher risk for type 2 diabetes[J].BMC Endocr Disord,2021,21(1):47.DOI:10.1186/s12902-021-00708-7.

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备注/Memo

备注/Memo:
基金项目:国家重点研发计划(2020YFA0803704); 国家自然科学基金(82070805,81870535); 天津市第一中心医院春风课题(2019CF37)
通信作者:刘尧娟,Email: liuyaojuan1983@163.com
更新日期/Last Update: 2021-10-10