[1]袁昕 高四书 罗凯明 项守奎 华飞.维生素D缺乏对雌性大鼠糖脂代谢及卵巢 形态功能的影响[J].国际内分泌代谢杂志,2019,39(01):7-11,16.[doi:10.3760/cma.j.issn.1673-4157.2019.01.002]
　Yuan Xin,Gao Sishu,Luo Kaiming,et al.Effects of vitamin D deficiency on glucolipid metabolism, morphology and function of ovary in female rats[J].International Journal of Endocrinology and Metabolism,2019,39(01):7-11,16.[doi:10.3760/cma.j.issn.1673-4157.2019.01.002]
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维生素D缺乏对雌性大鼠糖脂代谢及卵巢 形态功能的影响()
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《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
39
期数:
2019年01期
页码:
7-11,16
栏目:
论著
出版日期:
2019-01-20

文章信息/Info

Title:
Effects of vitamin D deficiency on glucolipid metabolism, morphology and function of ovary in female rats
作者:
袁昕 高四书 罗凯明 项守奎 华飞
苏州大学附属第三医院内分泌科,常州 213003
Author(s):
Yuan Xin;  Gao Sishu;  Luo Kaiming;  Xiang Shoukui;  Hua Fei
Department of Endocrinology, The Third Affiliated Hospital of Soochow University, Changzhou 213000, China
关键词:
维生素D;  大鼠;  甘油三酯;  胰岛素抵抗;  卵巢
Keywords:
Vitamin D;  Rat;  Triglyceride;  Insulin resistance;  Ovary
DOI:
10.3760/cma.j.issn.1673-4157.2019.01.002
摘要:
目的 研究维生素D缺乏对雌性大鼠糖、脂代谢及卵巢形态功能的影响。方法 48只3周龄Sprague-Dawley(SD)雌性大鼠按随机数字法随机分为对照组(n=24)和维生素D缺乏组(n=24),分别用标准饲料、维生素D缺乏饲料饲养。第6、14、22周每组按随机数字法选取8只,行阴道涂片观察动情周期14 d,于第8、16、24周处死并留取血清和卵巢组织。检测血清25-羟维生素D、血钙、血磷、空腹血糖、空腹胰岛素、甘油三酯、总胆固醇、睾酮、雌二醇、孕激素、黄体生成素(LH)、卵泡刺激素(FSH)、抗苗勒管激素水平,计算稳态模型评估-胰岛素抵抗指数(HOMA-IR)、LH/FSH比值,HE染色观察卵巢形态学改变。结果 第16、24周,与对照组相比,维生素D缺乏组甘油三酯、空腹血糖、空腹胰岛素、HOMA-IR、LH/FSH比值、抗苗勒管激素升高(t=-4.02～3.87,P均<0.05),第24周,与对照组相比,维生素D缺乏组FSH水平降低(t=2.35,P<0.05),卵巢出现多囊性改变。两组总胆固醇、睾酮、雌二醇、孕酮、动情周期差异均没有统计学意义(P均>0.05)。结论 维生素D缺乏可能影响雌性大鼠的糖、脂代谢以及卵巢形态和功能。
Abstract:
Objective To investigate the effects of vitamin D deficiency on glucolipid metabolism, morphology and function of ovary in female rats.Methods Forty-eight female Sprague-Dawley(SD)(3-week-old)rats were randomly divided into control group(n=24)and vitamin D deficiency group(n=24)according to random number method. All rats in control group were fed with standard diet and all rats in vitamin D deficiency group were fed with vitamin D deficient diet. On the 6th, 14th and 22th week 8 rats from each group were selected according to random number method and recieved the estrus cycle by vaginal smears for 14 days, they were sacrificed on the 8th, 16th, 24th week and serum as well as ovary specimens were collected. Serum 25(OH)D, calcium, phosphorus, fasting blood glucose, fasting insulin, triglyceride, total cholesterol, testosterone, estrogen, progestogen, luteinizing hormone(LH), follicle stimulating hormone(FSH)and anti-Müllerian hormone were measured. Homeostatic model assessment-insulin resistance(HOMA-IR)and LH/FSH ratio were calculated. Ovarian morphologic changes were observed by HE staining.Results On the 16th, 24th week, compared with control group, serum triglyceride, fasting blood glucose, fasting insulin, HOMA-IR, LH/FSH ratio, anti-Müllerian hormone levels were increased(t=-4.02-3.87, all P<0.05), while on the 24th week, compared with control group, the FSH level in vitamin D deficiency group was decreased(t=2.35, P<0.05), and ovarian polycystic alterations were observed in vitamin D deficiency group. No significant difference in serum total cholesterol, testosterone, estrogen, progestogen levels and the estrous cycle were found between the two groups(all P>0.05).Conclusion Vitamin D deficiency may affect glucolipid metabolism, morphology and function of ovary in female rats.

参考文献/References:

[1] Fischer V,Haffner-Luntzer M,Amling M,et al.Calcium and vitamin D in bone fracture healing and post-traumatic bone turnover[J].Eur Cell Mater,2018,35:365-385.DOI:10.22203/eCM.v035a25.
[2] Altieri B,Grant WB,Della Casa S,et al.Vitamin D and pancreas: the role of sunshine vitamin in the pathogenesis of diabetes mellitus and pancreatic cancer[J].Crit Rev Food Sci Nutr,2017,57(16):3472-3488.DOI:10.1080/10408398.2015.1136922.
[3] Degerud E,Nygård O,de Vogel S,et al.Plasma 25-hydroxyvitamin D and mortality in patients with suspected stable angina pectoris[J].J Clin Endocrinol Metab,2018,103(3):1161-1170.DOI:10.1210/jc.2017-02328.
[4] Wei Z,Yoshihara E,He N,et al.Vitamin D switches BAF complexes to protect β cells[J].Cell,2018,173(5):1135-1149.e15.DOI:10.1016/j.cell.2018.04.013.
[5] Nandi A,Sinha N,Ong E,et al.Is there a role for vitamin D in human reproduction?[J].Horm Mol Biol Clin Investig,2016,25(1):15-28.DOI:10.1515/hmbci-2015-0051.
[6] Sun W,Xie H,Ji J,et al.Defective female reproductive function in 1,25(OH)2D-deficient mice results from indirect effect mediated by extracellular calcium and/or phosphorus[J].Am J Physiol Endocrinol Metab,2010,299(6):E928-E935.DOI:10.1152/ajpendo.00378.2010.
[7] Lerchbaum E,Giuliani A,Gruber HJ,et al.Adult-type hypolactasia and calcium intake in polycystic ovary syndrome[J].Clin Endocrinol(Oxf),2012,77(6):834-843.DOI:10.1111/j.1365-2265.2012.04334.x.
[8] Stavenuiter AW,Arcidiacono MV,Ferrantelli E,et al. A novel rat model of vitamin D deficiency: safe and rapid induction of vitamin D and calcitriol deficiency without hyperparathyroidism[J].Biomed Res Int,2015,2015:604275.DOI:10.1155/2015/604275.
[9] Al-Dabhani K,Tsilidis KK,Murphy N,et al.Prevalence of vitamin D deficiency and association with metabolic syndrome in a Qatari population[J].Nutr Diabetes,2017,7(4):e263.DOI:10.1038/nutd.2017.14.
[10] Lu Y,Liu M,Pei Y,et al.Low levels of serum 25-hydroxyvitamin D and risk of metabolic syndrome in China[J].Int J Clin Exp Med,2015,8(8):13790-13796.
[11] Xue Y,Xu P,Xue K,et al.Effect of vitamin D on biochemical parameters in polycystic ovary syndrome women: a meta-analysis[J].Arch Gynecol Obstet,2017,295(2):487-496.DOI:10.1007/s00404-016-4247-y.
[12] Wehr E,Pieber TR,Obermayer-Pietsch B. Effect of vitamin D3 treatment on glucose metabolism and menstrual frequency in polycysticovary syndrome women: a pilot study[J].J Endocrinol Invest,2011,34(10):757-763.DOI:10.3275/7748.
[13] Tao S,Yuan Q,Mao L,et al.Vitamin D deficiency causes insulin resistance by provoking oxidative stress in hepatocytes[J].Oncotarget,2017,8(40):67605-67613.DOI:10.18632/oncotarget.18754.
[14] Yao X,Zhang G,Guo Y,et al.Vitamin D receptor expression and potential role of vitamin D on cell proliferation and steroidogenesis in goat ovarian granulosa cells[J].Theriogenology,2017,102:162-173.DOI:10.1016/j.theriogenology.2017.08.002.
[15] Hong SH,Lee JE,An SM,et al.Effect of vitamin D3 on biosynthesis of estrogen in porcine granulosa cells via modulation of steroidogenic enzymes[J].Toxicol Res,2017,33(1):49-54.DOI:10.5487/TR.2017.33.1.049.
[16] 中国医师协会内分泌代谢科医师分会. 多囊卵巢综合征诊治内分泌专家共识[J].中华内分泌代谢杂志, 2018, 34(1): 1-7.DOI:10.3760/cma.j.issn.1000-6699.2018.01.001.
[17] Bacopoulou F,Kolias E,Efthymiou V,et al.Vitamin D predictors in polycystic ovary syndrome: a meta-analysis[J].Eur J Clin Invest,2017,47(10):746-755.DOI:10.1111/eci.12800.
[18] Pal L,Zhang H,Williams J,et al.Vitamin D status relates to reproductive outcome in women with polycystic ovary syndrome: secondary analysis of a multicenter randomized controlled trial[J].J Clin Endocrinol Metab,2016,101(8):3027-3035.DOI:10.1210/jc.2015-4352.
[19] 何淑莹,中玉华,尤昭玲.多囊卵巢综合症LH及FSH的研究现状[J].中医药导报,2005, 11(8):82-84.DOI:10.3969/j.issn.1672-951X.2005.08.042.
[20] Kozakowski J,Kapus'cińska R,Zgliczyński W.Associations of vitamin D concentration with metabolic and hormonal indices in women with polycystic ovary syndrome presenting abdominal and gynoidal type of obesity[J].Ginekol Pol,2014,85(10):765-770.
[21] Irani M,Merhi Z.Role of vitamin D in ovarian physiology and its implication in reproduction: a systematic review[J].Fertil Steril,2014,102(2):460-468.e3.DOI:10.1016/j.fertnstert.2014.04.046.
[22] Merhi Z,Doswell A,Krebs K,et al.Vitamin D alters genes involved in follicular development and steroidogenesis in human cumulus granulosa cells[J].J Clin Endocrinol Metab,2014,99(6):E1137-E1145.DOI:10.1210/jc.2013-4161.

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备注/Memo

备注/Memo:
通信作者:华飞, Email:czhuafei@vip.sina.com
基金项目:常州市应用基础研究项目(CJ20159019,CJ20160046); 常州市卫生计生委指导性科技项目(wz201523)
Corresponding author: Hua Fei, Email:czhuafei@vip.sina.com
Fund program:Applied Basic Research Programs of Changzhou(CJ20159019,CJ20160046); Guiding Science and Technology Projects of Changzhou Health and Family Planning Commision(wz201523)
更新日期/Last Update: 2019-01-20