[1]梁栋,刘云峰,章毅,等.牛磺酸通过激活PI3K/Akt信号通路抑制高糖诱导的内皮细胞凋亡[J].国际内分泌代谢杂志,2015,(02):73-76.[doi:10.3760/cma.j.issn.1673-4157.2015.02.001]
　Liang Dong*,Liu Yunfeng,Zhang Yi,et al.Taurine inhibits endothelial cell apoptosis induced by high glucose through activating PI3K/Akt pathway[J].International Journal of Endocrinology and Metabolism,2015,(02):73-76.[doi:10.3760/cma.j.issn.1673-4157.2015.02.001]
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牛磺酸通过激活PI3K/Akt信号通路抑制高糖诱导的内皮细胞凋亡()
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《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
期数:
2015年02
页码:
73-76
栏目:
论著
出版日期:
2015-03-20

文章信息/Info

Title:
Taurine inhibits endothelial cell apoptosis induced by high glucose through activating PI3K/Akt pathway
作者:
梁栋; 刘云峰; 章毅; 尹建红; 许林鑫; 杨静
030001　太原，山西医科大学第一医院内分泌科（梁栋，刘云峰，尹建红，许林鑫，杨静）；030001　太原，山西医科大学基础医学院药理教研室（章毅）
Author(s):
Liang Dong*; Liu Yunfeng; Zhang Yi; Yin Jianhong; Xu Linxin; Yang Jing．
*Department of Endocrinology，The First Hospital of Shanxi Medical University，Taiyuan 030001，China Corresponding author：Yang Jing，Email：yangjlm@126.com
关键词:
牛磺酸; 内皮细胞; 磷脂酰肌醇3激酶; 蛋白激酶B; 细胞凋亡
Keywords:
Taurine; Endothelial cells; Phosphatidy linositol 3-kinase; Potein kinase B; Apoptosis
DOI:
10.3760/cma.j.issn.1673-4157.2015.02.001
摘要:
目的　探讨牛磺酸对高糖诱导的内皮细胞凋亡的影响及其信号途径。方法　取健康产妇分娩后12 h内的脐带分离人脐静脉内皮细胞（HUVEC），进行细胞培养并分为5 组：正常葡萄糖组（5 mmol/L D-葡萄糖，NG组）；高糖组（30 mmol/L D-葡萄糖，HG组）；NG+TAU组（5 mmol/L D-葡萄糖+ 5 mmol/L牛磺酸）；HG+TAU组（30 mmol/L D-葡萄糖+5 mmol/L牛磺酸）；HG+TAU+Wo组（30 mmol/L D-葡萄糖+5 mmol/L牛磺酸+50 nmol/L wortmannin）。干预48 h后应用流式细胞术检测细胞凋亡情况，Western印迹检测磷酸化蛋白激酶B （p-Akt）蛋白的表达水平，2，7-二氯二氢荧光素二乙酯（H2DCF-DA）探针技术及荧光倒置显微镜检测活性氧簇的相对含量。结果　与NG组相比，HG组细胞的凋亡率显著增加（P ＜0.05）。与HG组相比，HG+TAU组的凋亡率下降（P ＜0.05）。与HG+TAU组相比，HG+TAU+Wo组的凋亡率较高（P ＜0.05）。与NG组相比，HG组磷酸化-Akt蛋白表达下降（P ＜0.05）。与HG组比较，HG+TAU组磷酸化-Akt蛋白表达增加（P ＜0.05）。与HG+TAU组相比，HG+TAU+Wo组磷酸化-Akt蛋白表达下降（P ＜0.05）。牛磺酸可降低高糖诱导的活性氧簇生成（P ＜0.05），这一作用亦可被wortmannine所阻断（P ＜0.05）。结论　牛磺酸通过PI3K/Akt信号途径调节细胞内活性氧簇生成，进而在高糖环境下发挥其抗凋亡作用。
Abstract:
Objective　To observe the effects and signalling pathway of taurine on high glucose induced endothelial apoptosis. Methods　The umbilical cord from the healthy puerperal woman delivered in 12 hours was taken and the human umbilical vein endothelial cells（HUVECs）were isolated. HUVECs were cultured and divided into 5 groups：normal glucose group（5 mmol/L D-glucose, NG group），high glucose group（30 mmol/L D-glucose，HG group），NG+TAU group（5 mmol/L D-glucose+5 mmol/L taurine），HG+TAU group （30 mmol/L D-glucose+5 mmol/L taurine），HG+TAU+Wo group（30 mmol/L D-glucose+5 mmol/L taurine+ 50 nmol/L wortmannin）. After 48 hours，the apoptosis was tested by Flow Cytometry and expression of phospho-rylated-protein kinase B（p-Akt）was examined by Western blot. At the same time，the relative content of reactive oxygen species（ROS） was tested by 2'-7'-Dichlorofluorescein diacetate（H2DCF-DA） probe technique and fluorescent inverted microscope. Results　Compared with NG group，apoptosis rates in HG group were markedly enhanced（P＜0.05）.Compared with HG group，the apoptosis rates in HG+TAU group were decreased（P＜0.05）. Compared with HG+TAU group，apoptosis rates in HG+TAU+Wo group were increased（P＜0.05）.Compared with HG group，expression of p-Akt protein in NG+TAU group was increased（P＜0.05）. Compared with HG+TAU group，expression of p-Akt protein was reduced in HG+TAU+Wo group（P＜0.05）.Taurine could decrease the production of ROS induced by hyperglycemia（P＜0.05），and the effects were blocked by wortmannin（P＜0.05）.Conclusions　Taurine can regulate the generation of intracellular ROS by PI3K/Akt pathway，then prevent apoptosis of endothelial cell induced by high glucose.

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金资助项目（81373464，812708822）；山西省自然科学基金资助项目（2013011047-3 ,2012011039-8）；山西省卫生厅科研课题（201201062）；山西省回国留学人员科研资助项目（2013-111）；山西省留学人员择优启动项目（2011-762,2013-68）；中华医学会临床医学科研专项资金资助项目（13040440429） 通信作者：杨静，Email：yangjlm@126.com
更新日期/Last Update: 2015-03-20