[1]任国梅,任寿安.间歇低氧对大鼠肝脏GSK-3及mTOR表达的影响[J].国际内分泌代谢杂志,2015,(02):77-79,87.[doi:10.3760/cma.j.issn.1673-4157.2015.02.002]
　Ren Guomei,Ren Shouan..Effects of intermittent hypoxia on the expression of GSK-3 and mTOR in rat liver[J].International Journal of Endocrinology and Metabolism,2015,(02):77-79,87.[doi:10.3760/cma.j.issn.1673-4157.2015.02.002]
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间歇低氧对大鼠肝脏GSK-3及mTOR表达的影响()
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《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
期数:
2015年02
页码:
77-79,87
栏目:
论著
出版日期:
2015-03-20

文章信息/Info

Title:
Effects of intermittent hypoxia on the expression of GSK-3 and mTOR in rat liver
作者:
任国梅; 任寿安
030001　太原，山西医科大学第一医院呼吸科 　　
Author(s):
Ren Guomei; Ren Shouan.
Department of Respiration，The First Affiliated Hospital of Shanxi Medical University，Taiyuan 030001，China Corresponding author：Ren Shouan，Email：renshouan@163.com
关键词:
间歇低氧; 胰岛素抵抗; 糖原合成酶激酶-3; 哺乳动物雷帕霉素靶蛋白
Keywords:
Intermittent hypoxia; Insulin resistance; Glycogen synthase kinase-3; Mammalian target of rapamycin
DOI:
10.3760/cma.j.issn.1673-4157.2015.02.002
摘要:
目的　测定间歇低氧大鼠肝脏糖原合成酶激酶-3 （GSK-3）及哺乳动物雷帕霉素靶蛋白（mTOR）的表达，观察间歇低氧对胰岛素信号转导通路的影响。方法　将24只健康雄性Sprague- Dawley大鼠按照随机X表法分成间歇空气组（NC组）、间歇低氧4周组（IH4组）、间歇低氧8周组（IH8组），每组8只。于上午9:00至下午5:00将IH4组及IH8组暴露于间歇低氧舱内，NC组则给予间歇压缩空气。检测各组大鼠空腹血糖、空腹胰岛素（FINS），并以稳态模型评估-胰岛素敏感指数（HOMA-IS）及稳态模型评估- 胰岛素抵抗指数（HOMA-IR）评价胰岛素抵抗；免疫组化法测定大鼠肝脏GSK-3及mTOR的表达，以平均灰度值评价二者的蛋白表达量。结果　与NC组相比，IH4组、IH8组HOMA-IS降低，空腹血糖、FINS、HOMA-IR升高，以IH8组更为显著（F值分别为62.52，100.37，68.90， 85.49，P均＜0.01）；与NC组相比，IH4组、IH8组GSK-3及mTOR蛋白表达均升高，以IH8组更明显（F值分别为72.25，148.01，P均＜0.01）。Pearson相关分析显示GSK-3、mTOR平均灰度值与HOMA-IS呈正相关（r =0.786，0.811，P均＜0.01），与HOMA-IR呈负相关（r =-0.882，-0.889，P均＜0.01）。结论　间歇低氧暴露使大鼠肝脏GSK-3、mTOR表达增加，从而引起胰岛素抵抗。
Abstract:
Objective　To determine the expression of glycogen synthase kinase-3（GSK-3） and mammalian target of rapamycin（mTOR） in the liver of rats exposed to intermittent hypoxic，and to observe the effects of intermittent hypoxia on the insulin signaling pathway. Methods　Twenty four healthy male Sprague- Dawley rats were randomly divided into 3 groups according to the random number table: intermittent air group （NC group），4 weeks of intermittent hypoxia group（IH4 group） and 8 weeks of intermittent hypoxia group （IH8 group），with 8 rats in each group. Each day from 9:00 am to 5:00 pm，rats in NC group were housed in intermittent air cabin，while rats in IH4 group and IH8 group were housed in intermittent low oxygen cabin. Fasting blood glucose and fasting insulin（FINS） were tested. Homeostasis model assessment of insulin sensitive index（HOMA-IS） and homeostasis model assessment of insulin resistance index（HOMA-IR） were used to evaluate insulin resistance. The expression of hepatic GSK-3 and mTOR were determined by immunohistochemical method and the amount of protein expression was evaluated by average gray value. Results　Compared with NC group，HOMA-IS was reduced and fasting blood glucose，FINS，HOMA-IR were elevated in IH4 group and IH8 group，but was more apparent in IH8 group（F values were 62.52，100.375，68.90，85.49，all P＜0.01）. Compared with NC group， the expression of GSK-3 and mTOR protein were increased in IH4 group and IH8 group，especially in IH8 group（F values were 72.25，148.01，all P＜0.01）. Pearson correlation analysis showed that the average gray values of GSK-3 and mTOR were positively correlated to HOMA-IS（r =0.786，0.811，all P＜0.01），and negatively correlated with HOMA-IR（r =-0.882，-0.889，all P＜0.01）. Conclusions　Intermittent hypoxia increases the expression of GSK-3 and mTOR in the liver of rats，thus induces insulin resistance.

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备注/Memo

备注/Memo:
基金项目：山西科技研究基金资助项目（2013011048-4）　　 通信作者：任寿安，Email：renshouan@163.com
更新日期/Last Update: 2015-03-20