[1]周围 何萍 马鹏 杜冠华 强桂芬.内质网应激诱导小鼠棕色脂肪组织代谢紊乱的脂质组学研究[J].国际内分泌代谢杂志,2023,43(05):407-412.[doi:10.3760/cma.j.cn121383-20230724-07033]
 Zhou Wei,He Ping,Ma Peng,et al.Lipidomics study on ER stress-induced abnormal metabolism of brown adipose tissue in mice[J].International Journal of Endocrinology and Metabolism,2023,43(05):407-412.[doi:10.3760/cma.j.cn121383-20230724-07033]
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内质网应激诱导小鼠棕色脂肪组织代谢紊乱的脂质组学研究()
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《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
43
期数:
2023年05期
页码:
407-412
栏目:
论著
出版日期:
2023-09-20

文章信息/Info

Title:
Lipidomics study on ER stress-induced abnormal metabolism of brown adipose tissue in mice
作者:
周围1 何萍2 马鹏3 杜冠华3 强桂芬3
1国家药品监督管理局药品审评中心,北京 100022; 2中山大学附属第八医院,深圳 518033; 3北京协和医学院 中国医学科学院药物研究所药物靶点研究与新药筛选北京市重点实验室,北京 100050
Author(s):
Zhou Wei1 He Ping2 Ma Peng3 Du Guanhua3 Qiang Guifen3.
1Center for Drug Evaluation, National Medical Products Administration, Beijing 100022, China; 2The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen 518033; 3Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College and Beijing Key Laboratory of Drug Target and Screening Research, Beijing 100050, China
关键词:
棕色脂肪组织 内质网应激 脂质组学
Keywords:
Brown adipose tissue ER Stress Lipidomics
DOI:
10.3760/cma.j.cn121383-20230724-07033
摘要:
目的 探究内质网应激对棕色脂肪组织(BAT)脂质组成的影响。方法 雄性C57BL/6小鼠腹腔注射衣霉素溶液(2.5 mg/kg)18 h后取肩胛区BAT,采用超高效液相色谱(UPLC)仪串联SCIEX QTRAP 6500 PLUS质谱仪进行UPLC-MS/MS高覆盖率靶向脂质组学检测和分析。结果 内质网应激导致BAT脂质组成发生显著改变,其中溶血磷脂二酸(LBPA)总含量升高最显著,此外,17种脂质亚类也显著升高,1种脂质亚类显著降低。结论 内质网应激对BAT脂质组学整体有明显影响,其差异脂质代谢物有望为代谢性疾病的诊断与治疗提供潜在的靶点。
Abstract:
Objective To quantify the metabolic profile of BAT upon induction of endoplasmic reticulum(ER)stress.Methods Male C57BL/6 mice were intraperitoneally injected with tunicamycin solution(2.5 mg/kg)for 18 h and BAT from the interscapular region was quickly excised. Ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)analysis was performed using an UPLC coupled with Sciex QTRAP 6500 Plus.Results The lipid composition of BAT changed significantly following ER stress induction, with a notable increase in the total contents of lyso-bisphosphatidic acids(LBPA). Furthermore, 17 lipid species were significantly increased, and one was markedly decreased.Conclusion ER stress significantly alters the BAT lipidome, and its differential lipid metabolites are expected to provide potential lipid targets for the diagnosis and treatment of metabolic diseases.

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备注/Memo

备注/Memo:
通信作者:强桂芬,Email:qianggf@imm.ac.cn
基金项目:国家自然科学基金资助项目(82070877); 中国医学科学院创新工程重大协同创新项目(2021-I2M-1-005); 深圳市福田区卫生健康系统科研项目(FTWS2022057)
更新日期/Last Update: 2023-10-10