[1]赵静,李菲,晏蔚田,等.桥本甲状腺炎患者血浆外泌体miRNA表达谱研究[J].国际内分泌代谢杂志,2022,42(02):108-113.[doi:10.3760/cma.j.cn121383-20200911-09024]
　Zhao Jing,Li Fei,Yan Weitian,et al.Differential expression profiles of plasma exosomal miRNA in Hashimoto's thyroiditis patients[J].International Journal of Endocrinology and Metabolism,2022,42(02):108-113.[doi:10.3760/cma.j.cn121383-20200911-09024]
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桥本甲状腺炎患者血浆外泌体miRNA表达谱研究()
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《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
42
期数:
2022年02期
页码:
108-113
栏目:
论著
出版日期:
2022-03-20

文章信息/Info

Title:
Differential expression profiles of plasma exosomal miRNA in Hashimoto's thyroiditis patients
作者:
赵静1; 李菲2; 晏蔚田2; 肖瑶2; 魏军平2
1北京市西城区广外医院肾病科 100055; 2中国中医科学院广安门医院内分泌科 100053
Author(s):
Zhao Jing1;  Li Fei2;  Yan Weitian2;  Xiao Yao2;  Wei Junping2.
1Department of Nephrology, Beijing Xicheng Guangwai Hospital, Beijing 100055, China; 2Department of Endocrinology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China
关键词:
桥本甲状腺炎;  外泌体;  微小RNAs;  RNA测序
Keywords:
Hashimoto's thyroiditis;  Exosomal;  MicroRNAs;  RNA-Seq
DOI:
10.3760/cma.j.cn121383-20200911-09024
摘要:
目的 检测桥本甲状腺炎患者(Hashimoto's thyroiditis,HT)甲状腺功能正常组、HT合并亚临床甲状腺功能减退(SCH)组以及对照组外泌体微小RNAs(microRNAs,miRNAs),探讨HT病程中不同甲状腺状态下的miRNA差异表达谱及其相应的生物学功能。方法 选取HT甲状腺功能正常者、HT合并SCH患者及健康志愿者各3人,提取外周血浆外泌体进行miRNA高通量测序,统计分析3组间差异表达miRNA,并进行靶基因预测、基因本体功能(GO)及京都基因与基因组百科全书通路(KEGG pathway)分析。结果 得到75条差异表达miRNA(log2FC>1且Q-value≤0.001),数据挖掘后获得hsa-miR-324-3p、hsa-miR-370-3p、hsa-miR-423-5p、hsa-miR-532-3p等10条核心miRNA。GO分析表明,差异miRNA的靶基因主要参与细胞过程、生物调节、代谢过程等生物学过程,具备结合、催化活性、转录调节活性等分子功能。KEGG分析得到的通路主要为环磷酸腺苷(cAMP)信号通路、人类表皮生长因子受体(human epidermal growth factor receptor,ErbB)信号通路、缺氧诱导因子-1(hypoxia inducible factor-1,HIF-1)信号通路等。结论 HT患者血浆外泌体miRNA较健康人存在差异表达,其在甲状腺功能正常与SCH两种不同状态中也存在差异表达。hsa-miR-324-3p、hsa-miR-370-3p、hsa-miR-423-5p及hsa-miR-532-3p等miRNA可能通过cAMP、ErbB及HIF-1等信号通路在HT病程进展中发挥一定的作用。
Abstract:
Objective To detect the exosomal miRNA in the cases of Hashimoto's thyroiditis(HT)with euthyroidism, HT combined with subclinical hypothyroidism and healthy control, and to explore the differential expression profiles of miRNA in different thyroid states in the course of HT and their corresponding biological functions.Methods Three cases of HT with euthyroidism, 3 cases of HT with subclinical hypothyroidism and 3 cases of healthy volunteers were enrolled in this study. The miRNA were extracted from peripheral plasma of these 9 samples, then high-throughout miRNA sequencing technology was performed for screening differential expression profiles, which followed by target gene prediction, Gene ontology(GO)enrichment and Kyoto Encyclopedia of Genes and Genomes pathway(KEGG pathway)analysis.Results A total of 75 differentially expressed miRNAs were found(log2FC>1 and Q-value≤0.001), and 10 core miRNAs were obtained through data mining, including hsa-miR-324-3p, hsa-miR-370-3p, hsa-miR-423-5p, hsa-miR-532-3p, etc. The results of GO enrichment analysis showed that these differential miRNA targeted genes mainly participated in a variety of biological processes such as cellular process, biological regulation and metabolic processes. Besides, they were involved in binding, catalytic activity, transcription regulation activity and other molecular functions. cAMP signaling pathway, ErbB signaling pathway and HIF-1 signaling pathway were the main pathways obtained by enrichment analysis of KEGG pathway.Conclusion There are differential expression profiles of exosomal miRNA in HT patients, and also in the two different states of normal thyroid function and subclinical hypothyroidism. MiRNAs such as hsa-miR-324-3p, hsa-miR-370-3p, hsa-miR-423-5p and hsa-miR-532-3p may play an important role in the progression of HT through cAMP signaling pathway,ErbB signaling pathwa and HIF-1 signaling pathway.

参考文献/References:

[1] Ragusa F,Fallahi P,Elia G,et al.Hashimotos' thyroiditis:epidemiology,pathogenesis,clinic and therapy[J].Best Pract Res Clin Endocrinol Metab,2019,33(6):101367.DOI:10.1016/j.beem.2019.101367.
[2] 中华医学会内分泌学分会《中国甲状腺疾病诊治指南》编写组.中国甲状腺疾病诊治指南——甲状腺炎:慢性淋巴细胞性甲状腺炎[J].中华内科杂志,2008,47(9):784-785.DOI:10.3321/j.issn:0578-1426.2008.09.032.
[3] Shan Z,Chen L,Lian X,et al.Iodine status and prevalence of thyroid disorders after introduction of mandatory universal salt iodization for 16 years in China a cross-sectional study in 10 cities[J].Thyroid,2016,26(8):1125-1130.DOI:10.1089/thy.2015.0613.
[4] 中华医学会内分泌学分会.成人甲状腺功能减退症诊治指南[J].中华内分泌代谢杂志,2017,33(2):167-180.DOI:10.3760/cma.j.issn.1000-6699.2017.02.018.
[5] Weetman A.A hundred years of Hashimoto's thyroiditis[J].Thyroid,2013,23(2):135-136.DOI:10.1089/thy.2013.2302.ed1.
[6] Zhao L,Zhou X,Shan X,et al.Differential expression levels of plasma microRNA in Hashimoto's disease[J].Gene,2018,642:152-158.DOI:10.1016/j.gene.2017.10.053.
[7] Massolt ET,Chaker L,Visser TJ,et al.Serum microRNA profiles in athyroid patients on and off levothyroxine therapy[J].PLoS One,2018,13(4):e0194259.DOI:10.1371/journal.pone.0194259.
[8] Jiang W,Zheng L,Yan Q,et al.MiR-532-3p inhibits metastasis and proliferation of non-small cell lung cancer by targeting FOXP3[J].J BUON,2019,24(6):2287-2293.
[9] Song X,Wu R,Tao Q,et al.miR-532 promotes colorectal cancer invasion and metastasis by targeting NKD1[J].Cell Mol Biol(Noisy-le-grand),2019,65(6):52-55.
[10] Zou X,Li M,Huang Z,et al.Circulating miR-532-502 cluster derived from chromosome X as biomarkers for diagnosis of breast cancer[J].Gene,2020,722:144104.DOI:10.1016/j.gene.2019.144104.
[11] Wang Y,Yang Z,Wang L,et al.miR-532-3p promotes hepatocellular carcinoma progression by targeting PTPRT[J].Biomed Pharmacother,2019,109:991-999.DOI:10.1016/j.biopha.2018.10.145.
[12] Chen F,Feng Z,Zhu J,et al.Emerging roles of circRNA_NEK6 targeting miR-370-3p in the proliferation and invasion of thyroid cancer via Wnt signaling pathway[J].Cancer Biol Ther,2018,19(12):1139-1152.DOI:10.1080/15384047.2018.1480888.
[13] Tian D,Sha Y,Lu JM,et al.MiR-370 inhibits vascular inflammation and oxidative stress triggered by oxidized low-density lipoprotein through targeting TLR4[J].J Cell Biochem,2018,119(7):6231-6237.DOI:10.1002/jcb.26851.
[14] Zeng Y,Fu M,Wu GW,et al.Upregulation of microRNA-370 promotes cell apoptosis and inhibits proliferation by targeting PTEN in human gastric cancer[J].Int J Oncol,2016,49(4):1589-1599.DOI:10.3892/ijo.2016.3642.
[15] Ye W,Deng X,Fan Y.Exosomal miRNA423-5p mediated oncogene activity in papillary thyroid carcinoma:a potential diagnostic and biological target for cancer therapy[J].Neoplasma,2019,66(4):516-523.DOI:10.4149/neo_2018_180824N643.

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备注/Memo

备注/Memo:
通信作者:魏军平,Email:weijunping@126.com
基金项目:国家自然科学基金(81573961); 中央级公益性科研院所基本科研业务专项资金资助项目(ZZ11-030); 首都卫生发展科研专项(2014-2-4152,2020-2-4154)
更新日期/Last Update: 2022-04-20