[1]李宏淼 马连学 曾瑞霞 单伟.神经调节蛋白-1在高糖诱导的视网膜 Mller细胞凋亡中的作用[J].国际内分泌代谢杂志,2019,39(06):373-376.[doi:10.3760/cma.j.issn.1673-4157.2019.06.003]
　Li Hongmiao,Ma Lianxue,Zeng Ruixia,et al.Role of neuregulin-1 in the apoptosis of retinal Mller cells induced by high glucose[J].International Journal of Endocrinology and Metabolism,2019,39(06):373-376.[doi:10.3760/cma.j.issn.1673-4157.2019.06.003]
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神经调节蛋白-1在高糖诱导的视网膜 Müller细胞凋亡中的作用()
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《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
39
期数:
2019年06期
页码:
373-376
栏目:
论著
出版日期:
2019-11-20

文章信息/Info

Title:
Role of neuregulin-1 in the apoptosis of retinal Müller cells induced by high glucose
作者:
李宏淼1; 2 马连学2 曾瑞霞1 单伟1
1锦州医科大学基础医学院解剖学教研室 121001; 2辽宁省健康产业集团抚矿总医院(中国医科大学第七临床学院)检验科,抚顺 113008
Author(s):
Li Hongmiao1; 2;  Ma Lianxue1; 2;  Zeng Ruixia1;  Shan Wei1
1Department of Anatomy, Basic Medical College, Jinzhou Medical University, Jinzhou 121001, China; 2Department of Laboratory, The Fuming General Hospital of Liaoning Health Industry Group(The Seventh Clinical College of China Medical University), Fushun 113008, China
关键词:
神经调节蛋白-1;  细胞凋亡;  Mü; ller细胞;  氧化应激
Keywords:
Neuregulin-1;  Apoptosis;  Mü; ller cells;  Oxidative stress
DOI:
10.3760/cma.j.issn.1673-4157.2019.06.003
摘要:
目的 探讨神经调节蛋白-1(NRG-1)对高糖培养的视网膜Müller细胞凋亡的影响及机制。方法 将培养的Müller细胞分为对照组(空白对照)、高糖组(30 mmol/L葡萄糖)、NRG-1处理组(30 mmol/L葡萄糖+80 g/L NRG-1)。噻唑蓝法检测细胞活力,试剂盒检测丙二醛含量及超氧化物歧化(SOD)活力,流式细胞仪检测Müller细胞凋亡情况,Western印迹检测Bcl-2、Bax蛋白相对表达,JC-1检测细胞线粒体膜电位变化。结果 与对照组相比,高糖组细胞活力、SOD活性、Bcl-2表达及线粒体膜电位明显降低,丙二醛含量、Bax表达及细胞凋亡率明显增加(F=110.80～3 539.02, P均<0.01)。与高糖组相比,NRG-1处理组细胞活力、SOD活性、Bcl-2表达及线粒体膜电位明显增加,丙二醛含量、Bax表达及细胞凋亡率明显下降(F=110.80～3 539.02,P均<0.01)。结论 NRG-1可抑制高糖诱导的Müller细胞凋亡,其机制可能与抑制线粒体凋亡途径有关。
Abstract:
Objective To investigate the effect and mechanism of neuregulin-1(NRG-1)on apoptosis of retinal Müller cells cultured with high glucose.Methods The cultured Müller cells were divided into control group(blank control), high glucose group(30 mmol/L glucose)and NRG-1 treatment group(30 mmol/L glucose +80 g/L NRG-1). Cell viability was detected by methyl thiazolyl tetrazolium; malondialdehyde(MDA)content and superoxide dismutase(SOD)activity were detected by kit; the apoptosis of Müller cells was detected by flow cytometry; the relative expression of Bcl-2 and Bax protein were detected by Western blotting; mitochondrial membrane potential was detected by JC-1.Results Compared with control group, the cell viability, SOD activity, expression of Bcl-2 and mitochondrial membrane potential in high glucose group were decreased significantly; while the MDA content, expression of Bax and apoptotic rate of Müller cells were increased significantly(F=110.80-3 539.02, all P<0.01). Compared with high glucose group, the cell viability, SOD activity, expression of Bcl-2 and mitochondrial membrane potential in NRG-1 treatment group were increased significantly; while the MDA content, expression of Bax and the apoptotic rate of Müller cells were decreased significantly(F=110.80-3 539.02, all P<0.01).Conclusion NRG-1 can inhibit the apoptosis of Müller cells induced by high glucose, which may be related to the inhibition of mitochondrial apoptosis pathway.

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备注/Memo

备注/Memo:
通信作者:单伟,Email:604616912@qq.com
Corresponding author: Shan Wei, Email: 604616912@qq.com
基金项目:辽宁省教育厅自然科学基金(L2015313)
Fund program:Natural Science Foundation of Liaoning Provincial Department of Education(L2015313)
更新日期/Last Update: 2019-11-20