[1]张博 李凤君 左中夫.生长抑素对糖尿病大鼠视网膜病变的神经 保护作用及对GFAP、突触素表达的影响[J].国际内分泌代谢杂志,2018,38(06):370-373.[doi:10.3760/cma.j.issn.1673-4157.2018.06.003]
　Zhang Bo*,Li Fengjun,Zuo Zhongfu.The neuroprotection of somatostatin and its effects on the expression of GFAP and synaptophysin in rats with diabetic retinopathy[J].International Journal of Endocrinology and Metabolism,2018,38(06):370-373.[doi:10.3760/cma.j.issn.1673-4157.2018.06.003]
点击复制

生长抑素对糖尿病大鼠视网膜病变的神经 保护作用及对GFAP、突触素表达的影响()
分享到：

《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
38
期数:
2018年06期
页码:
370-373
栏目:
论著
出版日期:
2018-11-20

文章信息/Info

Title:
The neuroprotection of somatostatin and its effects on the expression of GFAP and synaptophysin in rats with diabetic retinopathy
作者:
张博 李凤君 左中夫
作者单位:125001 葫芦岛爱尔眼科医院综合眼科(张博); 063000 唐山市眼科医院青光眼科(李凤君); 121001 锦州医科大学解剖教研室(左中夫)
Author(s):
Zhang Bo*;  Li Fengjun;  Zuo Zhongfu
*Department of Ophthalmology, Huludao Ai'er Eye Hospital, Huludao 125001, China
关键词:
生长抑素;  糖尿病视网膜病变;  神经保护;  神经胶质酸性蛋白;  突触素
Keywords:
Somatostatin;  Diabetic retinopathy;  Neuroprotection;  Glial fibrillary acidic protein;  Synaptophysin
DOI:
10.3760/cma.j.issn.1673-4157.2018.06.003
摘要:
目的 探讨生长抑素对糖尿病大鼠视网膜病变的神经保护作用及对神经胶质酸性蛋白(GFAP)、突触素表达的影响。方法 清洁级雄性Sprague-Dawley(SD)大鼠24只,应用随机数字表法分成对照组、糖尿病组、生长抑素治疗组,每组8只。糖尿病组、生长抑素治疗组采用链脲佐菌素诱导糖尿病模型,模型诱导成功后,生长抑素治疗组给予腹腔注射鼠生长抑素(10 μg·kg-1·d-1)。对照组、糖尿病组给予等剂量生理盐水。12周后,HE染色检测视网膜神经节细胞(RGC)密度,免疫荧光检测视网膜GFAP、突触素表达,Western印迹检测视网膜GFAP、突触素蛋白的相对表达量。结果 与对照组相比,糖尿病组RGC密度下降,GFAP荧光强度升高,突触素荧光强度降低,GFAP蛋白表达升高,突触素表达降低(F=187.62、226.12、159.30、259.09、425.93,P均<0.01); 而与糖尿病组相比,生长抑素治疗组RGC密度增加,GFAP荧光强度降低,突触素荧光强度升高,GFAP表达下降,突触素表达增加(F=187.62、226.12、159.30、259.09、425.93,P均<0.01)。结论 生长抑素可能通过抑制胶质细胞活化,上调视网膜突触素表达,恢复视网膜RGC密度,对糖尿病视网膜病变具有神经保护作用。
Abstract:
Objective To investigate the neuroprotective effects of somatostatin and its effects on the expression of glial fibrillary acidic protein(GFAP)and synaptophysin(SYN)in rats with diabetic retinopathy.Methods A total of 24 clean male Sprague-Dawley(SD)rats were divided into control group, diabetic group and somatostatin treatment group according to random number table, with 8 rats in each group. Diabetes was induced by streptozotocin in diabetic group and somatostatin treatment group. After diabetes was induced, rats in somatostatin treatment group were given somatostatin(10 μg·kg-1·d-1)by intraperitoneal injection. Rats in control group and diabetic group were given the equal dose of saline. After 12 weeks, the density of retinal ganglion cells(RGC)were detected by HE staining; the expression of retinal GFAP and SYN were detected by immunofluorescence and Western blotting.Results Compared with control group, the fluorescence intensity of GFAP was significantly increased while the RGC density and the fluorescence intensity of SYN were decreased significantly. The expression of GFAP protein were increased and the expression of SYN protein was decreased in diabetic group compared with control group(F=187.62, 226.12, 159.30, 259.09, 425.93, all P<0.01). Compared with diabetic group, the fluorescence intensity of GFAP was decreased significantly while the RGC density and the fluorescence intensity of SYN were increased significantly. The expression of GFAP was decreased and the expression of SYN was increased in somatostatin treatment group compared with diabetic group(F=187.62, 226.12, 159.30, 259.09, 425.93, all P<0.01).Conclusion Somatostatin may increase the expression of SYN and restore the density of RGC by inhibiting the activation of glial cells, which suggests that somatostatin may play a neuroprotective role in diabetic retinopathy.

参考文献/References:


[1] Mozetic V,Freitas CG,Riera R.Statins and fibrates for diabetic retinopathy: protocol for a systematic review[J].JMIR Res Protoc,2017,6(2):e30. DOI:10.2196/resprot.6650.
[2] 赵红燕,李蓉.糖尿病个体化医疗的研究进展[J].国际内分泌代谢杂志,2017,37(3):174-177. DOI:10.3760/cma.j.issn.1673-4157.2017.03.008.
[3] Altmann C,Schmidt MHH.The role of microglia in diabetic retinopathy: inflammation, microvasculature defects and neurodegeneration[J].Int J Mol Sci,2018,19(1):pii: E110. DOI:10.3390/ijms19010110.
[4] Mazzeo A,Arroba AI,Beltramo E,et al.Somatostatin protects human retinal pericytes from inflammation mediated by microglia[J].Exp Eye Res,2017,164:46-54.DOI:10.1016/j.exer.2017.07.011.
[5] 孙亚东,马彦,姜艳静,等.生长抑素对糖尿病大鼠早期视网膜电图的影响[J]. 吉林大学学报(医学版), 2014(6):1192-1196. DOI:10.13481/j.1671-587x.20140614.
[6] Beltramo E,Lopatina T,Mazzeo A,et al.Effects of the neuroprotective drugs somatostatin and brimonidine on retinal cell models of diabetic retinopathy[J].Acta Diabetol,2016,53(6):957-964.DOI:10.1007/s00592-016-0895-4.
[7] Hernández C,García-Ramírez M,Corraliza L,et al.Topical administration of somatostatin prevents retinal neurodegeneration in experimental diabetes[J].Diabetes,2013,62(7):2569-2578.DOI:10.2337/db12-0926.
[8] Ng DS,Chiang PP,Tan G,et al.Retinal ganglion cell neuronal damage in diabetes and diabetic retinopathy[J].Clin Exp Ophthalmol,2016,44(4):243-250.DOI:10.1111/ceo.12724.
[9] Chohan A,Singh U,Kumar A,et al.Müller stem cell dependent retinal regeneration[J].Clin Chim Acta,2017,464:160-164.DOI:10.1016/j.cca.2016.11.030.
[10] Martinez-De Luna RI,Ku RY,Aruck AM,et al.Müller glia reactivity follows retinal injury despite the absence of the glial fibrillary acidicprotein gene in Xenopus[J].Dev Biol,2017,426(2):219-235.DOI:10.1016/j.ydbio.2016.03.005.
[11] Gudi V,Gai L,Herder V,et al.Synaptophysin is a reliable marker for axonal damage[J].J Neuropathol Exp Neurol,2017,[Epub ahead of print].DOI:10.1093/jnen/nlw114.
[12] D'Cruz TS,Weibley BN,Kimball SR,et al.Post-translational processing of synaptophysin in the rat retina is disrupted by diabetes[J].PLoS One,2012,7(9):e44711.DOI:10.1371/journal.pone.0044711.
[13] Ilha J,Centenaro LA,Broetto Cunha N,et al. The beneficial effects of treadmill step training on activity-dependent synaptic and cellular plasticity markers after complete spinal cord injury[J].Neurochem Res,2011,36(6):1046-1055.DOI:10.1007/s11064-011-0446-x.
[14] Zhou L,Wang H,Luo J,et al.Regulatory effects of inhibiting the activation of glial cells on retinal synaptic plasticity[J].Neural Regen Res,2014,9(4):385-393.DOI:10.4103/1673-5374.128240.

相似文献/References:

[1]苏月,王洁,于珮.血浆纤维蛋白原在糖尿病视网膜病变 患者中的水平及意义[J].国际内分泌代谢杂志,2016,36(01):1.[doi:10.3760/cma.j.issn.1673-4157.2016.01.001]
　Su Yue,Wang Jie,Yu Pei..Level of plasma fibrinogen in patients with diabetic retinopathy and its significance[J].International Journal of Endocrinology and Metabolism,2016,36(06):1.[doi:10.3760/cma.j.issn.1673-4157.2016.01.001]
[2]殷俏,张云良,郭淑芹,等.视黄醇结合蛋白4、超敏C反应蛋白 与糖尿病视网膜病变的关系[J].国际内分泌代谢杂志,2016,36(03):149.[doi:10.3760/cma.j.issn.1673-4157.2016.03.02]
　Yin Qiao,Zhang Yunliang,Guo Shuqin,et al.Relationship of retinol binding protein 4 and high sensitive C-reactive protein with diabetic retinopathy[J].International Journal of Endocrinology and Metabolism,2016,36(06):149.[doi:10.3760/cma.j.issn.1673-4157.2016.03.02]
[3]邵怡,王安平,王先令,等.肿瘤相关性低磷骨软化症的诊疗进展[J].国际内分泌代谢杂志,2017,37(02):112.[doi:10.3760/cma.j.issn.1673-4157.2017.02.011]
　Shao Yi,Wang Anping,Wang Xianling,et al.Diagnosis and treatment of tumor induced osteomalacia[J].International Journal of Endocrinology and Metabolism,2017,37(06):112.[doi:10.3760/cma.j.issn.1673-4157.2017.02.011]
[4]高璐,信中,袁明霞,等.代谢综合征与糖尿病及糖尿病前期人群 糖尿病视网膜病变的相关性分析[J].国际内分泌代谢杂志,2017,37(05):298.
　Gao Lu*,Xin Zhong,Yuan Mingxia,et al.A study on correlation between metabolic syndrome and diabetic retinopathy in diabetic and pre-diabetic people[J].International Journal of Endocrinology and Metabolism,2017,37(06):298.
[5]刘晓妮 王颖 李桃桃 马小敏 梁彦.血管紧张素转换酶基因rs4353多态性 与糖尿病微血管病变的相关性研究[J].国际内分泌代谢杂志,2018,38(03):149.[doi:10.3760/cma.j.issn.1673-4157.2018.03.002]
　Liu Xiaoni*,Wang Ying,Li Taotao,et al.Association between angiotensin converting enzyme gene rs4353 polymorphism and diabetic microangiopathy[J].International Journal of Endocrinology and Metabolism,2018,38(06):149.[doi:10.3760/cma.j.issn.1673-4157.2018.03.002]
[6]毕会利 张慧娟.信号素3家族与糖尿病及其微血管并发症[J].国际内分泌代谢杂志,2018,38(02):110.[doi:10.3760/cma.j.issn.1673-4157.2018.02.010]
　Bi Huili,Zhang Huijuan..Class 3 semaphorins in diabetes mellitus and its microvascular complications[J].International Journal of Endocrinology and Metabolism,2018,38(06):110.[doi:10.3760/cma.j.issn.1673-4157.2018.02.010]
[7]韩秀丹 朱凌燕 徐积兄.二甲双胍对糖尿病视网膜病变的作用及机制[J].国际内分泌代谢杂志,2019,39(01):53.[doi:10.3760/cma.j.issn.1673-4157.2019.01.013]
　Han Xiudan,Zhu Lingyan,Xu Jixiong.Effects of metformin on diabetic retinopathy and its mechanism[J].International Journal of Endocrinology and Metabolism,2019,39(06):53.[doi:10.3760/cma.j.issn.1673-4157.2019.01.013]
[8]俞冰鸿 杨菊红 单春艳.阻塞性睡眠呼吸暂停低通气综合征与糖尿病 视网膜病变的关系[J].国际内分泌代谢杂志,2019,39(04):269.[doi:10.3760/cma.j.issn.1673-4157.2019.04.013]
　Yu Binghong,Yang Juhong,Shan Chunyan.Relationship between obstructive sleep apnea syndrome and diabetic retinopathy[J].International Journal of Endocrinology and Metabolism,2019,39(06):269.[doi:10.3760/cma.j.issn.1673-4157.2019.04.013]
[9]孙梅 刘栩晗 王冰 高政南.吸烟与中老年糖尿病患者视网膜病变的 相关性研究[J].国际内分泌代谢杂志,2020,40(04):223.[doi:10.3760/cma.j.issn.1673-4157.2020.04.002]
　Sun Mei,Liu Xuhan,Wang Bing,et al.Study on the correlation between smoking and diabetic retinopathy in middle-age and old people[J].International Journal of Endocrinology and Metabolism,2020,40(06):223.[doi:10.3760/cma.j.issn.1673-4157.2020.04.002]
[10]黄庆先,胡文超,何兰杰.血清asprosin与糖尿病视网膜病变的 相关性研究[J].国际内分泌代谢杂志,2020,40(06):372.[doi:10.3760/cma.j.cn121383-20200311-03027]
　Huang Qingxian,Hu Wenchao,He Lanjie.Association of serum asprosin concentrations with diabetic retinopathy[J].International Journal of Endocrinology and Metabolism,2020,40(06):372.[doi:10.3760/cma.j.cn121383-20200311-03027]

备注/Memo

备注/Memo:
基金项目:中国博士后科学基金项目(2017M612870); 辽宁省高等学校杰出青年学者成长计划(LJQ2015069); 辽宁省自然科学基金(201602340)
Fund program:China Postdoctoral Science Foundation Project(2017M612870); Liaoning Provincial Outstanding Young Scholars Growth Plan(LJQ2015069); Natural Science Founda
更新日期/Last Update: 2018-12-30