[1]李新,孙家忠,孙苏欣,等.AMPK信号通路介导了CTRP3增加3T3-L1脂肪细胞脂联素表达的研究[J].国际内分泌代谢杂志,2014,(01):15-17.[doi:10.3760/cma.j.issn.1673-4157.2014.01.004]
　Li Xin,Sun Jiazhong,Sun Suxin,et al.Study of AMPK signal transduction mediated the expression of adiponectin in 3T3-L1 adipocytes increased by CTRP3[J].International Journal of Endocrinology and Metabolism,2014,(01):15-17.[doi:10.3760/cma.j.issn.1673-4157.2014.01.004]
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AMPK信号通路介导了CTRP3增加3T3-L1脂肪细胞脂联素表达的研究()
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《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
期数:
2014年01期
页码:
15-17
栏目:
论著
出版日期:
2014-02-28

文章信息/Info

Title:
Study of AMPK signal transduction mediated the expression of adiponectin in 3T3-L1 adipocytes increased by CTRP3
作者:
李新; 孙家忠; 孙苏欣; 杨杪; 吴玉文
430071　武汉大学中南医院内分泌科
Author(s):
Li Xin; Sun Jiazhong; Sun Suxin; Yang Miao; Wu Yuwen.
Department of Endocrinology,Zhongnan Hospital,Wuhan University,Wuhan 430071,China
关键词:
CTRP3; 3T3-L1脂肪细胞; 脂联素; AMPK
Keywords:
CTRP3; 3T3-L1 adipocytes; Adiponectin; AMPK
DOI:
10.3760/cma.j.issn.1673-4157.2014.01.004
摘要:
目的　探讨AMP活化蛋白激酶（AMPK）信号通路在C1q/肿瘤坏死因子相关蛋白3（CTRP3）调节3T3-L1脂肪细胞脂联素表达中的作用。方法　将3T3-L1脂肪细胞分为对照组、CTRP3（250 ?滋g/L）干预组及CTRP3（250 ?滋g/L）+ Compound C（10 ?滋mol/L 预处理1 h）干预组。以酶联免疫吸附法（ELISA）检测细胞培养上清中脂联素含量,实时定量PCR检测脂联素mRNA相对表达水平,Western印迹检测AMPK（thr172）蛋白相对表达量。结果　与对照组相比,CTRP3干预组脂联素mRNA表达及蛋白分泌分别增加53.0%（q =15.09,P＜0.01）及63.3%（q =8.11,P＜0.01）,AMPK（thr172）表达增加43.0%（q =14.64,P＜0.01）；与CTRP3干预组相比,CTRP3+Compound C干预组AMPK（thr172）蛋白的表达降低57.3%（q =27.92,P＜0.01）,脂联素mRNA表达及蛋白分泌分别下降26.1%（q =11.39,P＜0.01）及54.1%（q =11.31,P＜0.01）。结论　AMPK信号通路参与了CTRP3对3T3-L1脂肪细胞脂联素表达的调控过程。
Abstract:
Objective　To investigate the role of AMP activated protein kinase（AMPK） signal in the modulation of C1q/TNF related protein 3（CTRP3） on the expression of adiponectin in adipocytes.Methods 　The 3T3-L1 adipocytes were divided into control group,CTRP3（250 ?滋g/L） intervention group and CTRP3（250 ?滋g/L）+ Compound C（10 ?滋mol/L pretreated for 1 h） intervention group.The content of adiponectin in supernatant was detected by enzyme linked immunosorbent assay.The relative expression of adiponectin mRNA was detected by real-time polymerase chain reaction.The relative expression of AMPK（thr172） was detected by Western blot．Results　Compared with control group, expression of adiponectin mRNA and protein increased 53.0% （q =15.09,P＜0.01） and 63.3% （q =8.11,P＜0.01） respectively, the expression of AMPK（thr172） increased 43.0% （q =14.64,P＜0.01） in CTRP3 intervention group.Compared with CTRP3 intervention group,the expression of AMPK（thr172） in CTRP3+ Compound C intervention group decreased 57.3% （q =27.92,P＜0.01）,expression of adiponectin mRNA and protein decreased 26.1%（q =11.39,P＜0.01） and 54.1% （q =11.31,P＜0.01） respectively．Conclusion　AMPK signal participates in the modulation of CTRP3 on the expression of adiponectin in 3T3-L1 adipocytes.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目：中央高校基本科研业务专项资金（4101017）
更新日期/Last Update: 2014-01-20