[1]周晓丽,张倩,张大龙,等.富硒酵母对高脂血症大鼠脂代谢的影响[J].国际内分泌代谢杂志,2017,37(06):361-366.
　Zhou Xiaoli*,Zhang Qian,Zhang Dalong,et al.Effects of selenium enriched yeast on lipid metabolism in rats with hyperlipidemia[J].International Journal of Endocrinology and Metabolism,2017,37(06):361-366.
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富硒酵母对高脂血症大鼠脂代谢的影响()
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《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
37
期数:
2017年06期
页码:
361-366
栏目:
论著
出版日期:
2017-11-20

文章信息/Info

Title:
Effects of selenium enriched yeast on lipid metabolism in rats with hyperlipidemia
作者:
周晓丽; 张倩; 张大龙; 李倩; 张经纬; 周朋辉; 张静; 张书婧; 钱智勇; 顾清
300011 天津市疾病预防控制中心毒理科(周晓丽,张倩,张大龙,李倩,张静,张书婧,钱智勇),党委办公室(张经纬),传染病科(周朋辉,顾清)
Author(s):
Zhou Xiaoli*;  Zhang Qian;  Zhang Dalong;  Li Qian;  Zhang Jingwei;  Zhou Penghui;  Zhang Jing;  Zhang Shujing;  Qian Zhiyong;  Gu Qing.
*Department of Toxicology, Tianjin Centers for Disease Control and Prevention, Tianjin 300011, China
关键词:
富硒酵母;  高脂血症;  血脂;  胆固醇
Keywords:
Selenium enriched yeast;  Hyperlipidemia;  Blood lipid;  Cholesterol
文献标志码:
A
摘要:
目的 探讨富硒酵母对高脂血症大鼠脂代谢的影响及调节机制。方法 将40只雄性Sprague-Dawley(SD)大鼠按体重随机区组分组法分为空白对照组、模型对照组(HF组)和低剂量富硒酵母组(HF+LSe组,硒剂量为 0.83 μg/kg)、高剂量富硒酵母组(HF+HSe组,硒剂量为8.33 μg/kg),每组10只。空白对照组正常饮食,其他组高脂饮食。实验期为90 d,其中造模30 d,干预60 d。干预期间每周记录动物进食量和体重,并计算食物利用率。结束时(即干预第60天)全自动生化仪检测丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST),计算血清动脉粥样硬化指数(AI)和血浆致动脉粥样硬化指数(AIP)。全部动物进行大体解剖,取肝组织HE染色和油红O染色观察肝组织病理学形态,并制作 10%肝匀浆测肝组织中胆固醇和甘油三酯水平。应用实时PCR检测羟甲基戊二酰辅酶A还原酶(HMG-CoA-R)、低密度脂蛋白受体(LDLR)、酰基辅酶A-胆固醇酰基转移酶(ACAT)和卵磷脂胆固醇脂酰转移酶(LCAT)的mRNA表达水平。结果 HF+LSe组在干预第56天平均体重显著低于HF组(F=3.028,P<0.05)。HF+HSe组及HF+LSe组的进食量和总增重均显著低于HF组(F=19.706、5.776,P均<0.05),HF+LSe组总食物利用率显著低于HF组(F=23.791,P<0.05)。HF+LSe组和HF+HSe组的肝脏总胆固醇、甘油三酯、AI均明显低于HF组(F=55.413、5.322、42.405,P均<0.05)。HF+LSe组的AIP显著低于HF组(F=9.522, P<0.05)。HF+HSe组的肝功能指标ALT和HF+LSe组的AST均显著低于HF组(F=5.942、3.489,P均<0.05)。与HF组相比,HF+HSe组和HF+LSe组肝细胞均有不同程度的修复,以HF+LSe组修复效果最佳。HF+LSe组的HMG-CoA-R mRNA表达水平与HF组比较显著降低(F=5.972,P<0.05)。结论 富硒酵母可以抑制高脂饲料引起的大鼠体重增加,具有降血脂和抗动脉粥样硬化作用; 对高脂血症大鼠的肝脏组织具有明显的修复功能。其主要通过下调HMG-CoA-R mRNA表达水平,调节脂代谢。
Abstract:
Objective To investigate the effects and the underlying mechanisms of selenium enriched yeast on hyperlipidemia in high-fat diet(HF)-fed rats.Methods Forty male Sprague-Dawley(SD)rats were divided into four groups according to their weight by random block grouping method: blank control group, model group(HF group), low dose of selenium enriched yeast intervention group(HF+LSe group, selenium dose 0.83 μg/kg)and high dose of selenium enriched yeast intervention group(HF+HSe group, selenium dose 8.33 μg/kg), 10 rats for each group. Rats in blank control group were fed with normal diet while the other rats were fed with high-fat diet. The whole study lasted for 90 days including 30 days of modeling phase and 60 days of intervention phase. During intervention, weight and food intake were recorded weekly, and the food utilization rate was calculated. At the end of the experiment(the 60th day of intervention), alanine aminotransferase, aspartate aminotransferase were measured by automatic biochemical analyzer. Serum atherosclerosis index(AI)and plasma induced atherosclerosis index(AIP)were calculated. All animals were dissected to observe the morphological changes of liver by HE and Oil Red O staining. The 10% hepatic tissue homogenates were prepared to detect the contents of total cholesterol(TC)and triglyceride(TG).3-Hydroxy-3-methylglutaryl coenzyme A reductase(HMG-CoA-R), low-density lipoprotein receptor(LDLR), acyl coenzyme A cholesterol acyltransferase(ACAT)and lecithin cholesterol acyltransferase(LCAT)mRNA were analyzed by real-time reverse transcription polymerase chain reaction.Results The average body weight in HF+LSe group was significantly lower than that of HF group on 56th day of intervention(F=3.028, P<0.05). The food intake and total weight gain in HF+HSe group and HF+LSe group were significantly lower than those in HF group(F=19.706, 5.776, all P<0.05), whereas the total food utilization of HF+LSe group was significantly lower than that in HF group(F=23.791, P<0.05). Liver TC, TG and AI of HF+HSe group and HF+LSe group were significantly lower than those in HF group(F=55.413, 5.322, 42.405,all P<0.05). AIP in HF+LSe group was significantly lower than that in HF group(F=9.522, P<0.05). ALT in HF+HSe group and AST in HF+LSe group were significantly lower than those in HF group(F=5.942, 3.489, all P<0.05). Compared with HF group, HF+HSe group and HF+LSe group had different levels of repair in hepatic cells, especially in HF+LSe group. The expression of HMG-CoA-R mRNA level in HF+LSe group was decreased significantly compared with HF group(F=5.972, P<0.05).Conclusions Selenium enriched yeast inhibits the weight gain of rats induced by high fat diet, possesses the effect of lowering blood lipid and anti-atherosclerosis, and repairs the liver tissue of hyperlipidemia rats. The main mechanism of selenium enriched yeast on lipid regulation may be its inhibition of HMG-CoA-R mRNA expression.

参考文献/References:

[1] Surhio MM, Wang Y, Xu P,et al. Antihyperlipidemic and hepatoprotective properties of selenium modified polysaccharide from Lachnum sp[J].Int J Biol Macromol,2017,99:88-95. DOI:10.1016/j.ijbiomac.2017.01.148.
[2] Laclaustra M, Stranges S, Navas-Acien A,et al. Serum selenium and serum lipids in US adults: National Health and Nutrition Examination Survey(NHANES)2003-2004[J].Atherosclerosis,2010,210(2):643-648. DOI:10.1016/j.atherosclerosis.2010.01.005.
[3] 张经纬,张静姝,周朋辉,等.富硒酵母对高脂饮食大鼠血脂和抗氧化指标的影响[J].中国慢性病预防与控制,2016,24(3):216-218.DOI:10.16386/j.cjpccd.issn.1004-6194.2016.03.016.
[4] Nagi MN, Almakki HA, Sayed-Ahmed MM,et al. Thymoquinone supplementation reverses acetaminophen-induced oxidative stress, nitric oxide production and energy decline in mice liver[J].Food Chem Toxicol,2010,48(8-9):2361-2365. DOI:10.1016/j.fct.2010.05.072.
[5] Schimmel EK, Yazici Y. Increased lipid levels but unchanged atherogenic index in rheumatoid arthritis patients treated with biologic disease modifying antirheumatic drugs: published experience[J].Clin Exp Rheumatol,2009,27(3):446-451.
[6] Dobiásová M. Atherogenic index of plasma [log(triglycerides/HDL-cholesterol)]: theoretical and practical implications[J].Clin Chem,2004,50(7):1113-1115. DOI:10.1373/clinchem.2004.033175.
[7] Al-Tai WF, Jaffer EMH, Ali ZA. Evaluating the utility of plasma atherogenic index among several atherogenic parameters in patients with chronic renal failure on maintenance hemodialysis[J]. J Fac Med Baghdad,2012,54(3):259-262.
[8] Ezeukwu AO, Agwubike EO. Anthropometric measures of adiposity as correlates of atherogenic index of plasma in non-obese sedentary Nigerian males[J].Libyan J Med,2014,9(1):23798. DOI:10.3402/ljm.v9.23798.
[9] Dobiásová M, Frohlich J. The plasma parameter log(TG/HDL-C)as an atherogenic index:correlation with lipoprotein particle size and esterification rate in apoB-lipoprotein-depleted plasma(FER(HDL))[J].Clin Biochem,2001,34(7):583-588.
[10] Nido SA, Shituleni SA, Mengistu BM,et al. Effects of selenium-enriched probiotics on lipid metabolism, antioxidative status, histopathological lesions, and related gene expression in mice fed a high-fat diet[J].Biol Trace Elem Res,2016,171(2):399-409. DOI:10.1007/s12011-015-0552-8.
[11] 王东亮,肖敏,钱卫,等. 细菌还原氧化态硒产生红色单质硒的研究进展[J]. 微生物学报,2007,47(3): 554-557.DOI:10.3321/j.issn:0001-6209.2007.03.037.
[12] 李万立,张守华,查龙应,等. 富硒酵母对铜负荷导致小鼠肝损伤的保护作用[J]. 营养学报,2009,31(5):486-489.DOI:10.3321/j.issn:0512-7955.2009.05.017.
[13] Sreekala S, Indira M. Effect of exogenous selenium on nicotine induced hyperlipidemia in rats[J].Indian J Physiol Pharmacol,2008,52(2):132-140.
[14] Dhingra S, Bansal MP. Hypercholesterolemia and LDL receptor mRNA expression: modulation by selenium supplementation[J].Biometals,2006,19(5):493-501. DOI:10.1007/s10534-005-5393-z.
[15] Dhingra S, Bansal MP. Modulation of hypercholesterolemia-induced alterations in apolipoprotein B and HMG-CoA reductase expression by selenium supplementation[J].Chem Biol Interact,2006,161(1):49-56. DOI:10.1016/j.cbi.2006.02.008.
[16] Goldstein JL, Brown MS. Regulation of the mevalonate pathway[J].Nature,1990,343(6257):425-430. DOI:10.1038/343425a0.
[17] Shefer S, Hauser S, Lapar V,et al. HMG CoA reductase of intestinal mucosa and liver of the rat[J].J Lipid Res,1972,13(3):402-412.

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备注/Memo

备注/Memo:
基金项目:天津市卫生行业重点攻关项目(13KG138)
通信作者:顾清,Email:guqing315@126.com
更新日期/Last Update: 2017-11-30