[1]周晓丽 孙毅娜 钱智勇 顾清 张倩 李晶.硒联合维生素B6对高脂血症患者 血脂水平的影响及机制研究[J].国际内分泌代谢杂志,2019,39(01):1-6.[doi:10.3760/cma.j.issn.1673-4157.2019.01.001]
　Zhou Xiaoli,Sun Yina,Qian Zhiyong,et al.Effects and mechanism of selenium combined with vitamin B6 on the level of blood lipid in patients with hyperlipidemia[J].International Journal of Endocrinology and Metabolism,2019,39(01):1-6.[doi:10.3760/cma.j.issn.1673-4157.2019.01.001]
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硒联合维生素B6对高脂血症患者 血脂水平的影响及机制研究()
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《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
39
期数:
2019年01期
页码:
1-6
栏目:
论著
出版日期:
2019-01-20

文章信息/Info

Title:
Effects and mechanism of selenium combined with vitamin B6 on the level of blood lipid in patients with hyperlipidemia
作者:
周晓丽1 孙毅娜2 钱智勇1 顾清3 张倩1 李晶2
1天津市疾病预防控制中心毒理科 300011; 2国家卫生健康委员会激素与发育重点实验室(天津医科大学),天津市代谢性疾病重点实验室,天津医科大学代谢病医院内分泌研究所 300070; 3天津市疾病预防控制中心传染病科 300011
Author(s):
Zhou Xiaoli1;  Sun Yina2;  Qian Zhiyong1;  Gu Qing3;  Zhang Qian1;  Li Jing2.
1Department of Toxicology, Tianjin Center for Disease Control and Prevention, Tianjin 300011, China; 2NHC Key Laboratory of Hormone and Development(Tianjin Medical University), Tianjin Key Laboratory of Metabolic Diseases, The Metabolic Diseases Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin 300070, China; 3Department of Infectious Diseases, Tianjin Center for Disease Control and Prevention, Tianjin 300011, China
关键词:
硒联合维生素B6;  高脂血症;  脂代谢
Keywords:
Selenium combined with vitamin B6;  Hyperlipidemia;  Lipid metabolism
DOI:
10.3760/cma.j.issn.1673-4157.2019.01.001
摘要:
目的 研究硒联合维生素B6对高脂血症人群血脂的作用及脂代谢的影响,并探讨其调节机制。方法 将2017年6-12月体检查出的高脂血症患者100例,按照区组随机方法分为对照组50例,试验组50例。试验组口服富硒酵母维生素E片(硒含量50 μg/d)和维生素B6片(10 mg/d),对照组服用普通酵母维生素E,连续干预30 d。分别于试验第0天、第30天对全部受试者进行常规体格检查(包括血常规与血生化检查),测定血清硒和维生素B6含量以及血清总胆固醇、甘油三酯、低密度脂蛋白-胆固醇(LDL-C)和高密度脂蛋白-胆固醇(HDL-C)水平; 于试验第30天,检测试验组与对照组血清3-羟基-3-甲基戊二酰辅酶A还原酶(HMGR)、肝脂酶、脂蛋白脂肪酶(LPL)和总脂酶的水平,并采用荧光定量PCR法测定受试者全血中沉默信息调节因子1(SIRT1)、过氧化物酶体增殖物活化受体α(PPARα)和固醇调节元件结合蛋白-1c(SREBP-1c)mRNA的表达水平。结果干预第0天、第30天,试验组各项体检指标与对照组相比,差异均无统计学意义(P均>0.05),与第0天相比,对照组和试验组第30天血清白蛋白降低(t=5.942、6.162,P均<0.05),其他各项体检指标均未见显著改变(P均>0.05); 试验组第30天血清总胆固醇和LDL-C水平低于对照组第30天及自身第0天水平(t=2.960、2.990,P均<0.05; t=2.190、2.086,P均<0.05); 第30天,与对照组相比,试验组血清HMGR水平降低,肝脂酶、LPL以及总脂酶水平升高(t=5.839、-2.072、3.115、-2.323,P均<0.05)。试验组全血SIRT1和PPARα mRNA表达水平高于对照组,SREBP-1c mRNA表达水平低于对照组(t=6.295、7.662、6.857,P均<0.05); 试验组第30天血清硒和维生素B6水平高于对照组第30天及自身第0天水平(t=-2.606、-2.710,P均<0.05; t=-4.296、-3.690,P均<0.05)。结论 适量补充富硒酵母和维生素B6能够显著降低高脂血症人群血清总胆固醇和LDL-C水平,作用机制可能与二者调节SIRT1、PPARα和SREBP-1c等关键脂代谢调节因子的表达水平以及脂代谢酶HMGR、肝脂酶和LPL的分泌水平有关。
Abstract:
Objective To study the effects of selenium combined with vitamin B6 on blood lipid and lipid metabolism in patients with hyperlipidemia, and to explore its regulatory mechanism.Methods A total of 100 patients with hyperlipidemia detected by physical examination during June 2017 to December 2017 were divided into control group and experimental group according to stratified-block random method,with 50 cases in each group. Patients in experimental group took the selenium(50 μg/d)enriched yeast vitamin E tablets and vitamin B6 tablets(10 mg/d). Patients in control group took vitamin E tablets with ordinary yeast. Both of them were treated for 30 days. The routine physical examination(including rounting blood test and blood biochemical examination)was performed on the 0th day and the 30th day, respectively. Serum level of selenium, vitamin B6, total cholesterol, triglyceride, low density lipoprotein-cholesterol(LDL-C)and high density lipoprotein-cholesterol(HDL-C)were measured. On the 30th day of the experiment, the levels of serum 3-hydroxyl-3-methylamyl two coenzyme A reductase(HMGR), liver lipase, lipoprotein lipase(LPL)and total lipase were detected in both groups. The mRNA level of silencing information regulator 1(SIRT1), peroxisome proliferator-activated receptor α(PPARα)and sterol regulatory element binding protein-1c(SREBP-1c)in the whole blood were analyzed by real-time transcription polymerase chain reaction.Results The physical examination indexes of experimental group were not different from those in control group on the 0th day and the 30th day after the intervention(all P>0.05). Compared with the 0th day, the level of serum albumin in control group and experimental group on the 30th day were decreased(t=5.942, 6.162, all P<0.05). Other physical examination indexes of control group and experimental group were not changed on the 30th day(all P>0.05). The serum level of total cholesterol and LDL-C on the 30th day in experimental group were lower than those of control group and the 0th day when compared with itself(t=2.960, 2.990, all P<0.05; t=2.190, 2.086, all P<0.05). On the 30th day, compared with control group, the serum level of HMGR was decreased but the liver lipase, LPL and total lipase levels were increased(t=5.839,-2.072, 3.115,-2.323,all P<0.05). The level of SIRT1 and PPARα mRNA in the whole blood of experimental group were higher than those of control group, but the level of SREBP-1c mRNA was lower than that of control group(t=6.295, 7.662, 6.857, all P<0.05). The serum level of selenium and vitamin B6 in experimental group were higher than those of control group at the 30th day and the 0th day when compared with itself(t=-2.606, -2.710, all P<0.05; t=-4.296, -3.690, all P<0.05).Conclusions Moderate supplementation of selenium enriched yeast combined with vitamin B6 can reduce the level of serum total cholesterol and LDL-C among people with hyperlipidemia. The mechanism may be caused by the regulation of the expression of key lipid metabolic regulators such as SIRT1, PPARα and SREBP-1c, as well as the secretion of lipid metabolism enzymes such as HMGR, liver lipase and LPL.

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相似文献/References:

[1]周晓丽,张倩,张大龙,等.富硒酵母对高脂血症大鼠脂代谢的影响[J].国际内分泌代谢杂志,2017,37(06):361.
　Zhou Xiaoli*,Zhang Qian,Zhang Dalong,et al.Effects of selenium enriched yeast on lipid metabolism in rats with hyperlipidemia[J].International Journal of Endocrinology and Metabolism,2017,37(01):361.

备注/Memo

备注/Memo:
通信作者:李晶,Email:2003-victor@163.com; 顾清,Email:guqing315@126.com
基金项目:天津市卫生行业重点攻关项目(13KG138)
Corresponding author: Li Jing, Email:2003-victor@163.com; Gu Qing, Email:guqing315@126.com
Fund program:Key Research Project of Tianjin Health Industry(13KG138)
更新日期/Last Update: 2019-01-20