[1]李吉隆,周杰,王宝利.microRNA-200c对3T3-L1前体脂肪细胞 向脂肪细胞分化的调控作用[J].国际内分泌代谢杂志,2017,37(02):73-76.[doi:10.3760/cma.j.issn.1673-4157.2017.02.001]
　Li Jilong,Zhou Jie,Wang Baoli..The regulatory effects of microRNA-200c on the differentiation of 3T3-L1 preadipocytes to adipocytes[J].International Journal of Endocrinology and Metabolism,2017,37(02):73-76.[doi:10.3760/cma.j.issn.1673-4157.2017.02.001]
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microRNA-200c对3T3-L1前体脂肪细胞 向脂肪细胞分化的调控作用()
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《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
37
期数:
2017年02期
页码:
73-76
栏目:
论著
出版日期:
2017-03-20

文章信息/Info

Title:
The regulatory effects of microRNA-200c on the differentiation of 3T3-L1 preadipocytes to adipocytes
作者:
李吉隆; 周杰; 王宝利
300070 天津医科大学代谢病医院内分泌研究所,卫生部激素与发育重点实验室
Author(s):
Li Jilong;  Zhou Jie;  Wang Baoli.
Key Laboratory of Hormones and Development(Ministry of Health), The Metabolic Diseases Hospital & Institute of Endocrinology, Tianjin Medical University, Tianjin 300070, China
关键词:
微小RNA;  脂肪细胞;  细胞分化;  microRNA-200c
Keywords:
microRNA;  Adipocyte;  Cell differentiation;  microRNA-200c
DOI:
10.3760/cma.j.issn.1673-4157.2017.02.001
摘要:
目的 探讨microRNA(miRNA)-200c对3T3-L1前体脂肪细胞分化的影响。方法 利用实时PCR检测miRNA-200c在小鼠骨髓间充质干细胞(MSCs)向脂肪细胞分化过程及向骨细胞分化过程中的表达。通过将miRNA-200c类似物、miRNA-200c抑制剂转染至3T3-L1前体脂肪细胞,从而在细胞中增强或抑制miRNA-200c的表达,油红O染色检测转染后3T3-L1前体脂肪细胞诱导成熟后的脂滴形成情况。实时PCR检测转染后脂肪细胞特异性转录因子过氧化物酶体增殖物活化受体γ(PPARγ)和表征基因aP2在成脂过程中的表达变化。结果 实时PCR结果显示,在小鼠骨髓MSCs诱导成脂后miRNA-200c表达升高(t=24.709, P<0.01),而诱导成骨后,miRNA-200c表达下降(t=8.783, P<0.01)。转染miRNA-200c 类似物后,3T3-L1前体脂肪细胞中脂滴明显增多,PPARγ和aP2表达显著升高(t=7.674、9.657,P均<0.01); 转染miRNA-200c抑制剂后,3T3-L1前体脂肪细胞中脂滴明显减少,PPARγ、aP2表达显著降低(t=9.483、6.419, P均<0.01)。结论 miRNA-200c能够促进3T3-L1前体脂肪细胞向脂肪细胞分化。
Abstract:
Objective To investigate the effects of microRNA(miRNA)-200c on the differentiation of 3T3-L1 preadipocytes. Methods Real-time PCR was used to examine the expression of miRNA-200c in mouse bone marrow-derived mesenchymal stem cells(MSCs)during adipogenic differentiation and osteogenic differentiation. Oil-red O staining was used to measure the formation of oil droplets in 3T3-L1 preadipocytes cells after the transfection of miRNA-200c mimics or inhibitor. Real-time PCR was used to analyze the expression of adipocyte-specific transcription factors peroxisome proliferator-activated receptor γ(PPARγ)and aP2 mRNA in adipogenic differentiation. Results Results from the real-time PCR revealed that the expression of miRNA-200c was increased after the adipogenic differentiation in mouse bone marrow-derived MSCs(t=24.709, P<0.01), and was decreased after the osteogenic differentiation(t=8.783, P<0.01). Transfection of miRNA-200c mimics promoted the accumulation of lipid droplets and up-regulated the expression of PPARγ and aP2 mRNA in 3T3-L1 preadipocytes(t=7.674, 9.657, all P<0.01). Inversely, miRNA-200c inhibitor suppressed the lipid droplets accumulation and down-regulated the expression of PPARγ and aP2 mRNA in 3T3-L1 preadipocytes(t=9.483, 6.419, all P<0.01). Conclusion miRNA-200c promotes adipogenic differentiation of 3T3-L1 preadipocytes.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81271977,81472040) 通信作者:王宝利,Email:bliwang72@163.com
更新日期/Last Update: 2017-03-20