[1]田清武,孙树凯,翟玉娥,等.雌激素对去卵巢大鼠内脏脂肪细胞脂肪因子表达水平的影响[J].国际内分泌代谢杂志,2016,36(06):374-379.[doi:10.3760/cma.j.issn.1673-4157.2016.06.04]
　Tian Qingwu*,Sun Shukai,Zhai Yue,et al.Effects of estrogen on the expression of adipokines in visceral fat cells in ovariectomized rats[J].International Journal of Endocrinology and Metabolism,2016,36(06):374-379.[doi:10.3760/cma.j.issn.1673-4157.2016.06.04]
点击复制

雌激素对去卵巢大鼠内脏脂肪细胞脂肪因子表达水平的影响()
分享到：

《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
36
期数:
2016年06期
页码:
374-379
栏目:
论著
出版日期:
2016-11-20

文章信息/Info

Title:
Effects of estrogen on the expression of adipokines in visceral fat cells in ovariectomized rats
作者:
田清武; 孙树凯; 翟玉娥; 赵鹏; 赵涵; 申井利; 翟木绪
266555 青岛大学附属医院检验科(田清武,孙树凯,翟玉娥,赵鹏,申井利),病理科(赵涵); 255000 淄博市妇幼保健院内分泌科(翟木绪)
Author(s):
Tian Qingwu*;  Sun Shukai;  Zhai Yu’e;  Zhao Peng;  Zhao Han;  Shen Jingli;  Zhai Muxu.
Department of Laboratory, The Affiliated Hospital of Qingdao University, Qingdao 266555, China
关键词:
卵巢切除;  脂肪组织;  脂肪因子
Keywords:
Ovariectomy;  Adipose tissue;  Adipokine
DOI:
10.3760/cma.j.issn.1673-4157.2016.06.04
摘要:
目的 观察雌激素对去卵巢大鼠内脏脂肪细胞瘦素、脂联素、抵抗素和肿瘤坏死因子-α(TNF-α)表达水平的影响,探讨雌激素对体脂分布的影响机制。方法 6周龄Sprauge-Dawley雌性大鼠30只,采用随机数字表法分成3组:假手术组、去卵巢组和去卵巢+戊酸雌二醇组(OVX+E2组),每组10只。术后1周,OVX+E2组大鼠每天按1 mg/kg体重灌胃戊酸雌二醇水溶液,其他组大鼠灌胃等体积蒸馏水。连续给药12周后,腹主动脉取血,迅速剥离内脏脂肪组织。采用全自动生化分析仪检测血脂、血糖。采用免疫组化染色、实时荧光定量RT-PCR和Western印迹检测脂肪细胞瘦素、脂联素、抵抗素和TNF-α的表达。结果 3组血清瘦素、脂联素和抵抗素水平差异无统计学意义(P均>0.05), 但去卵巢组TNF-α水平显著高于假手术组(F=4.785,P<0.05)。免疫组化显示,与假手术组相比,去卵巢组内脏脂肪组织中瘦素表达明显减弱,而脂联素、抵抗素和TNF-α表达明显增强; 与去卵巢组相比,OVX+E2组内脏脂肪组织中瘦素表达明显增强,脂联素、抵抗素和TNF-α表达明显减弱(F=3.712～5.198,P均<0.05)。3组内脏脂肪细胞瘦素mRNA和蛋白表达水平差异无统计学意义(P均>0.05); 去卵巢组内脏脂肪细胞脂联素、抵抗素和TNF-α的mRNA和蛋白表达水平显著高于假手术组,而OVX+E2组内脏脂肪细胞脂联素、抵抗素和TNF-α的mRNA和蛋白表达水平显著低于去卵巢组(F=3.175～5.342,P均<0.05)。结论 雌激素可通过下调去卵巢大鼠内脏脂肪细胞脂联素、抵抗素和TNF-α的表达,进而影响去卵巢大鼠体脂再分布。
Abstract:
Objective To observe the effects of estrogen on the expression of leptin, adiponectin, resistin and tumor necrosis factor-α(TNF-α)in visceral fat cells in ovariectomized rats and to investigate the mechanism of estrogen on body fat distribution. Methods A total of 30 female Sprauge-Dawley(SD)rats(6 weeks)were divided into three groups by random number table method: sham operation group, ovariectomy group and ovariectomy combined with estrogen treatment group(OVX+E2 group), with 10 rats in each group. One week after surgery, rats in OVX+E2 group were gavaged with estradiol valerate(1 mg/kg)whereas rats in the other groups were gavaged with the same volume of distilled water per day for 12 weeks. Blood was obtained from abdominal aorta, and then the visceral adipose tissue was quickly removed. Blood glucose and lipids were measured by full automatic biochemical analyzer. Expression of leptin, adiponectin, resistin and TNF-α were detected by immunocytochemichal staining, real-time fluorescence quantitative RT-PCR and Western blotting, respectively. Results There were no significant differences in serum leptin, adiponectin and resistin among three groups(all P>0.05), but serum TNF-α was significantly higher in ovariectomy group than that in sham operation group(F=4.785,P<0.05). Immunohistochemical staining showed that the expression of leptin in visceral adipose tissue of ovariectomy group was significantly decreased, and adiponectin, resistin and TNF-α were increased greatly compared with those in sham operation group. The expression of leptin was enhanced, whereas expression of adiponectin, resistin and TNF-α were significantly decreased in OVX+E2 group compared with those in ovariectomy group(F=3.712-5.198, all P<0.05).There were no significant differences in leptin mRNA and protein expression in visceral adipocyte among three groups(all P>0.05). The mRNA and protein of adiponectin, resistin and TNF-α were significantly higher in visceral fat cells of OVX+ E2 group than those of sham operation group, and were significantly lower in visceral fat cells of OVX+E2 group compared with those of ovariectomy group(F=3.175-5.342, all P<0.05). Conclusion Estrogen affects the redistribution of body fat in ovariectomized rats through downregulating the expression of adiponectin, resistin and TNF-α in visceral fat cells.

参考文献/References:

[1] Toth MJ, Tchernof A, Sites CK,et al. Menopause-related changes in body fat distribution[J].Ann N Y Acad Sci, 2000,904:502-506.
[2] Tchernof A, Poehlman ET. Effects of the menopause transition on body fatness and body fat distribution[J].Obes Res,1998,6(3):246-254.
[3] Lobo RA. Metabolic syndrome after menopause and the role of hormones[J].Maturitas, 2008,60(1):10-18. DOI: 10.1016/j.maturitas.2008.02.008.
[4] Clegg DJ. Minireview: the year in review of estrogen regulation of metabolism[J].Mol Endocrinol, 2012,26(12):1957-1960. DOI: 10.1210/me.2012-1284.
[5] Pantalea～o TU, Mousovich F, Rosenthal D,et al. Effect of serum estradiol and leptin levels on thyroid function, food intake andbody weight gain in female Wistar rats[J].Steroids, 2010,75(10):638-642. DOI: 10.1016/j.steroids.2010.03.009.
[6] Weigt C, Hertrampf T, Zoth N,et al. Impact of estradiol, ER subtype specific agonists and genistein on energy homeostasis in a rat model of nutrition induced obesity[J].Mol Cell Endocrinol, 2012,351(2):227-238. DOI: 10.1016/j.mce.2011.12.013.
[7] Kumar R, McEwan IJ. Allosteric modulators of steroid hormone receptors: structural dynamics and gene regulation[J].Endocr Rev, 2012,33(2):271-299. DOI: 10.1210/er.2011-1033.
[8] Lu X, Peng L, Lv M,et al. Recent advance in the design of small molecular modulators of estrogen-related receptors[J].Curr Pharm Des, 2012,18(23):3421-3431.
[9] Nilsson M, Dahlman I, Rydén M,et al. Oestrogen receptor alpha gene expression levels are reduced in obese compared to normal weight females[J].Int J Obes(Lond), 2007,31(6):900-907. DOI:10.1038/sj.ijo.0803528.
[10] Okura T, Koda M, Ando F,et al. Association of polymorphisms in the estrogen receptor alpha gene with body fat distribution[J].Int J Obes Relat Metab Disord, 2003,27(9):1020-1027.DOI:10.1038/sj.ijo.0802378.
[11] Mauvais-Jarvis F. Estrogen and androgen receptors: regulators of fuel homeostasis and emerging targets for diabetes and obesity[J].Trends Endocrinol Metab, 2011,22(1):24-33. DOI: 10.1016/j.tem.2010.10.002.
[12] Mauvais-Jarvis F, Clegg DJ, Hevener AL. The role of estrogens in control of energy balance and glucose homeostasis[J].Endocr Rev, 2013,34(3):309-338. DOI: 10.1210/er.2012-1055.
[13] Stirone C, Duckles SP, Krause DN,et al. Estrogen increases mitochondrial efficiency and reduces oxidative stress in cerebral blood vessels[J].Mol Pharmacol, 2005,68(4):959-965.DOI: 10.1124/mol.105.014662.
[14] Waki H, Tontonoz P. Endocrine functions of adipose tissue[J].Annu Rev Pathol, 2007,2:31-56.DOI:10.1146/annurev.pathol.2.010506.091859.
[15] Karim R, Stanczyk FZ, Brinton RD,et al. Association of endogenous sex hormones with adipokines and ghrelin in postmenopausal women[J].J Clin Endocrinol Metab, 2015,100(2):508-515. DOI: 10.1210/jc.2014-1839.
[16] Sato H, Ishikawa M, Sugai H,et al. Sex hormones influence expression and function of peroxisome proliferator-activated receptor γ in adipocytes: pathophysiological aspects[J].Horm Mol Biol Clin Investig, 2014,20(2):51-61. DOI: 10.1515/hmbci-2014-0026.
[17] Fungfuang W, Terada M, Komatsu N,et al. Effects of estrogen on food intake, serum leptin levels and leptin mRNA expression in adipose tissue of female rats[J].Lab Anim Res, 2013,29(3):168-173. DOI: 10.5625/lar.2013.29.3.168.
[18] Machinal F, Dieudonne MN, Leneveu MC,et al. In vivo and in vitro ob gene expression and leptin secretion in rat adipocytes: evidence for a regional specific regulation by sex steroid hormones[J].Endocrinology, 1999,140(4):1567-174.DOI:10.1210/endo.140.4.6617.
[19] Van Sinderen ML, Steinberg GR, J?rgensen SB,et al. Effects of estrogens on adipokines and glucose homeostasis in female aromatase knockout mice[J].PLoS One, 2015,10(8):e0136143. DOI: 10.1371/journal.pone.0136143.
[20] Combs TP, Berg AH, Rajala MW, et al. Sexual differentiation, pregnancy, calorie restriction, and aging affect theadipocyte-specific secretory protein adiponectin[J].Diabetes, 2003,52(2):268-276.
[21] Iwasa T, Matsuzaki T, Matsui S,et al. The effects of ovariectomy and LPS-induced endotoxemia on resistin levels in female rats[J].Cytokine, 2015,76(2):558-560. DOI: 10.1016/j.cyto.2015.06.003.
[22] Huang SW, Seow KM, Ho LT,et al. Resistin mRNA levels are downregulated by estrogen in vivo and in vitro[J].FEBS Lett, 2005,579(2):449-454.DOI:10.1016/j.febslet.2004.12.010.
[23] Titov VN. Phylogenesis of function of trophology. Functional difference between visceral fat cells and subcutaneous adipocytes[J].Vopr Pitan,2015,84(1):15-24.

相似文献/References:

[1]赵紫琴,雒瑢,田凤石,等.替米沙坦对OLETF大鼠皮下和内脏脂肪组织PPARγ表达的影响[J].国际内分泌代谢杂志,2014,(06):365.[doi:10.3760/cma.j.issn.1673-4157.2014.06.002]
　Zhao Ziqin*,Luo Rong,Tian Fengshi,et al.Effects of telmisartan on expression of PPARγ in subcutaneous and visceral adipose tissue in OLETF rats[J].International Journal of Endocrinology and Metabolism,2014,(06):365.[doi:10.3760/cma.j.issn.1673-4157.2014.06.002]
[2]杨曦,刘玉洁,马慧娟.C1q/肿瘤坏死因子相关蛋白12与糖代谢[J].国际内分泌代谢杂志,2016,36(03):195.[doi:10.3760/cma.j.issn.1673-4157.2016.03.13]
　Yang Xi*,Liu Yujie,Ma Huijuan..C1q/tumor necrosis factor-related protein 12 and glucose metabolism[J].International Journal of Endocrinology and Metabolism,2016,36(06):195.[doi:10.3760/cma.j.issn.1673-4157.2016.03.13]
[3]徐传翀,尚文斌.脂肪固有免疫细胞与胰岛素抵抗[J].国际内分泌代谢杂志,2016,36(04):257.[doi:10.3760/cma.j.issn.1673-4157.2016.04.11]
　Xu Chuanchong,Shang Wenbin.Innate immune cells in adipose tissue and insulin resistance[J].International Journal of Endocrinology and Metabolism,2016,36(06):257.[doi:10.3760/cma.j.issn.1673-4157.2016.04.11]
[4]龚凡,陆灏.IKKε调节代谢平衡及其机制[J].国际内分泌代谢杂志,2017,37(02):120.[doi:10.3760/cma.j.issn.1673-4157.2017.02.013]
　Gong Fan,Lu Hao..IKKε regulate energy balance and its mechanism[J].International Journal of Endocrinology and Metabolism,2017,37(06):120.[doi:10.3760/cma.j.issn.1673-4157.2017.02.013]
[5]王艳立,张林,胡茂清.长链非编码RNAs与脂肪组织的关系[J].国际内分泌代谢杂志,2017,37(02):131.[doi:10.3760/cma.j.issn.1673-4157.2017.02.016]
　Wang Yanli*,Zhang Lin,Hu Maoqing..Relationship between long noncoding RNAs and adipose tissue[J].International Journal of Endocrinology and Metabolism,2017,37(06):131.[doi:10.3760/cma.j.issn.1673-4157.2017.02.016]
[6]高晶扬,张曼娜,曲伸.microRNA与脂肪组织慢性低度炎性反应[J].国际内分泌代谢杂志,2017,37(02):135.[doi:10.3760/cma.j.issn.1673-4157.2017.02.017]
　Gao Jingyang,Zhang Manna,Qu Shen..microRNA and chronic low-grade inflammation of adipose tissue[J].International Journal of Endocrinology and Metabolism,2017,37(06):135.[doi:10.3760/cma.j.issn.1673-4157.2017.02.017]
[7]许瀚元 朱惠娟 龚凤英.恒定自然杀伤细胞与肥胖[J].国际内分泌代谢杂志,2019,39(01):40.[doi:10.3760/cma.j.issn.1673-4157.2019.01.010]
　Xu Hanyuan,Zhu Huijuan,Gong Fengying.Invariant natural killer T cells and obesity[J].International Journal of Endocrinology and Metabolism,2019,39(06):40.[doi:10.3760/cma.j.issn.1673-4157.2019.01.010]
[8]许瀚元 朱惠娟 龚凤英.成纤维细胞生长因子1与糖脂代谢[J].国际内分泌代谢杂志,2019,39(03):190.[doi:10.3760/cma.j.issn.1673-4157.2019.03.011]
　Xu Hanyuan,Zhu Huijuan,Gong Fengying.Fibroblast growth factor 1 and glucolipid metabolism[J].International Journal of Endocrinology and Metabolism,2019,39(06):190.[doi:10.3760/cma.j.issn.1673-4157.2019.03.011]
[9]杨佳苗 沈山梅.水通道蛋白7与脂肪组织代谢的关系[J].国际内分泌代谢杂志,2019,39(04):253.[doi:10.3760/cma.j.issn.1673-4157.2019.04.009]
　Yang Jiamiao,Shen Shanmei.Association between aquaporin 7 and adipose metabolism[J].International Journal of Endocrinology and Metabolism,2019,39(06):253.[doi:10.3760/cma.j.issn.1673-4157.2019.04.009]
[10]刘雨平,罗佐杰.促甲状腺激素受体在非甲状腺组织中的表达及意义[J].国际内分泌代谢杂志,2021,41(01):10.[doi:10.3760/cma.j.cn121383-20200509-05015]
　Liu Yuping,Luo Zuojie..Expression and significance of thyroid stimulating hormone receptor in extrathyroidal tissues[J].International Journal of Endocrinology and Metabolism,2021,41(06):10.[doi:10.3760/cma.j.cn121383-20200509-05015]

备注/Memo

备注/Memo:
基金项目:青岛经济技术开发区重点科技发展计划项目(2013-1-83)
更新日期/Last Update: 2016-12-20