[1]褚月颉,郑从从,丁敏,等.运动对后肢缺血糖尿病大鼠骨骼肌血管新生及Amot表达的影响[J].国际内分泌代谢杂志,2014,(04):220-224.[doi:10.3760/cma.j.issn.1673-4157.2014.04.002]
　Chu Yuejie,Zheng Congcong,Ding Min,et al.Effects of exercises on skeletal muscle angiogenesis and Amot expression in diabetic rats with ischemic hindlimbs[J].International Journal of Endocrinology and Metabolism,2014,(04):220-224.[doi:10.3760/cma.j.issn.1673-4157.2014.04.002]
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运动对后肢缺血糖尿病大鼠骨骼肌血管新生及Amot表达的影响()
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《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
期数:
2014年04期
页码:
220-224
栏目:
论著
出版日期:
2014-08-31

文章信息/Info

Title:
Effects of exercises on skeletal muscle angiogenesis and Amot expression in diabetic rats with ischemic hindlimbs
作者:
褚月颉; 郑从从; 丁敏; 徐俊; 王鹏华
作者单位：300070　天津医科大学代谢病医院足病科，卫生部激素与发育重点实验室 　　
Author(s):
Chu Yuejie; Zheng Congcong; Ding Min; Xu Jun; Wang Penghua.
Department of Diabetic Foot，The Metabolic Diseases Hospital，Tianjin Medical University，Key Laboratory of Hormones and Development，Ministry of Health，Tianjin 300070，China Corresponding author：Wang Penghua，Email： wph200000@163.com
关键词:
运动; 糖尿病; 缺血; 血管新生; 血管抑素结合蛋白
Keywords:
Exercise; Diabetes mellitus; Ischemia; Angiogenesis; Angiomotin
DOI:
10.3760/cma.j.issn.1673-4157.2014.04.002
摘要:
目的　观察运动对后肢缺血糖尿病大鼠骨骼肌血管新生及血管抑素结合蛋白（Amot）表达的影响，评价运动治疗对糖尿病下肢动脉闭塞的可能作用机制。方法　64只Sprague-Dawley（SD）大鼠按随机数字表法分为4组：即非糖尿病非缺血组（n =16），非糖尿病缺血组（n =16），糖尿病非缺血组（n =16），糖尿病缺血组（n =16），分别建模。每组各取8只大鼠进行定量运动，另外8只为非运动对照。免疫组化方法检测各组大鼠后肢骨骼肌血管密度（MVD），Western印迹方法测定各组大鼠后肢骨骼肌Amot表达。结果　在非运动状态下，与非糖尿病非缺血组大鼠相比，糖尿病非缺血组MVD降低[（14.0±1.4）vs. （16.9±1.3） 个/mm2，P ＜0.05]；与非糖尿病缺血组相比，糖尿病缺血组MVD降低[（17.5±1.4）vs.（24.9±1.2）个/mm2，P ＜0.001]；与非糖尿病非缺血组大鼠相比，非糖尿病缺血组MVD升高[（24.9±1.2）vs.（16.9±1.3）个/mm2，P ＜0.001]；与糖尿病非缺血组相比，糖尿病缺血组MVD升高[（17.5±1.4）vs.（14.0±1.4）个/mm2，P ＜0.01]。运动的各组大鼠与相应的非运动组比较，MVD均明显升高（P 均＜0.01）。运动的糖尿病缺血组与非运动的非糖尿病缺血组大鼠MVD相近（P ＞0.05）。在非运动状态下，缺血诱导骨骼肌p80-Amot表达升高，p130-Amot表达不变或降低。与非糖尿病非缺血组大鼠相比，非糖尿病缺血组大鼠p80-Amot表达升高（P ＜0.001），p130-Amot表达的改变无统计学意义（P ＞0.05）；与糖尿病非缺血组大鼠相比，糖尿病缺血组p80-Amot表达升高（P ＜0.01）， p130-Amot表达降低（P ＜0.01）。与非糖尿病非缺血组大鼠相比，糖尿病非缺血组p80-Amot表达改变无统计学意义（P ＞0.05），p130-Amot表达升高（P ＜0.001）；与非糖尿病缺血组大鼠相比，糖尿病缺血组p80-Amot表达降低（P ＜0.05），p130-Amot表达升高（P ＜0.001）。运动诱导大鼠骨骼肌p80/p130-Amot比值升高。骨骼肌p80/p130-Amot比值与MVD呈显著相关（r =0.977，P ＜0.01）。结论　运动能够刺激糖尿病缺血组织的血管新生，p80/p130-Amot比值是血管新生的良好预测因素。
Abstract:
Objective　To investigate the effects of exercises on skeletal muscle angiogenesis and Amot expression in diabetic rats with ischemic hindlimbs，in order to evaluate the mechanism of exercise treatment for arterial occlusion in lower extremity in diabetic patients. Methods　Sixty-four Sprague-Dawley （SD） rats were divided into four groups according to a random number table：non-diabetes and non-ischemia group （n =16），non-diabetes and ischemia group（n =16），diabetes and non-ischemia group（n =16），diabetes and ischemia group（n =16）. Rats in each group were modeled separately. Eight rats from each group were subjected to quantified movement，and other eight rats were used as the non-exercise control. Immunohisto-chemical staining was used to determine the muscle vascular density （MVD） of the hind limb skeletal muscle in different groups. Western blot was used to determine the Amot expression of the hind limb skeletal muscle in different groups. Results　In non-exercise rats，compared with non-diabetes and non-ischemia group，rats in diabetes and non-ischemia group had a lower MVD[（14.0±1.4） vs. （16.9±1.3） /mm2，P ＜0.05]. Compared with non-diabetes and ischemia group，rats in diabetes and ischemia group had a lower MVD [(917.5±1.4) vs.（24.9±1.2）/mm2， P ＜0.001]. Compared with non-diabetes and non-ischemia group，rats in non-diabetes and ischemia group had a higher MVD [（24.9±1.2）vs.（16.9±1.3）/mm2， P ＜0.001]. Compared with diabetes and non-ischemia group，rats in diabetes and ischemia group had a higher MVD [（17.5±1.4） vs.（14.0±1.40/mm2， P ＜0.01]. Rats sujected to exercise had a significantly elevated MVD than the corresponding non-exercise rats（all P ＜0.01）. Rats sujected to exercise and in diabetes and ischemia group had a equal MVD to that in non-diabetes and ischemia group but undertaken no exercise [ （25.9±1.1）vs.（24.9±1.2）/mm2， P ＞0.05]. In the non-exercise rats，ischemia induced an increased expression of p80-Amot but an unchanged or decreased expression of p130-Amot in skeletal muscle. Compared with non-diabetes and non-ischemia group， rats in non-diabetes and ischemia group had a higher p80-Amot expression（P ＜0.001）. There was no significant change in p130-Amot expression（P ＞0.05）. Compared with diabetes and non-ischemia group， rats in diabetes and ischemia group had a higher p80-Amot expression （P ＜0.01）， and a lower p130-Amot expression（P ＜0.01）. Compared with non-diabetes and non-ischemia group， there was no significant change in p80-Amot expression in diabetes and non-ischemia group（P ＞0.05），while there was a higher p130-Amot expression in diabetes and non-ischemia group（P ＜0.001）. Compared with non-diabetes and ischemia group，rats in diabetes and ischemia had a lower p80-Amot expression（P ＜0.05），and a higher p130-Amot expression（P ＜0.001）. However，p80/p130-Amot ratio in the skeletal muscle was increased in the exercise rats. There was a significant correlation between the p80/p130-Amot ratio and MVD in the skeletal muscle（r =0.977， P ＜0.01）. Conclusions　Exercises can stimulate angiogenesis in the ischemic tissue of diabetic rats. The p80/p130-Amot ratio is a good predictor of angiogenesis.

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备注/Memo

备注/Memo:
基金项目：天津市应用基础及前沿技术研究计划项目（天津市自然科学基金重点项目）（10JCZDJC19800）；天津医科大学科学基金（2008KY17）；天津市卫生局科技基金（09KZ87） 通信作者：王鹏华，Email:wph200000@163.com
更新日期/Last Update: 2014-07-20