[1]惠宗光,周学伟,李文通,等.β3肾上腺素能受体基因多态性与糖尿病足的相关性研究[J].国际内分泌代谢杂志,2014,(02):138-140.[doi:10.3760/cma.j.issn.1673-4157.2014.02.019]
　Hui Zongguang*,Zhou Xuewei,Li Wentong,et al.Relationship between gene polymorphism of beta 3-adrenergic receptor and diabetic foot[J].International Journal of Endocrinology and Metabolism,2014,(02):138-140.[doi:10.3760/cma.j.issn.1673-4157.2014.02.019]
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β3肾上腺素能受体基因多态性与糖尿病足的相关性研究()
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《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
期数:
2014年02期
页码:
138-140
栏目:
短篇论著
出版日期:
2014-04-30

文章信息/Info

Title:
Relationship between gene polymorphism of beta 3-adrenergic receptor and diabetic foot
作者:
惠宗光; 周学伟; 李文通; 孙金红; 侯宁宁; 孙红喜
261041　潍坊医学院附属医院内分泌科（惠宗光,孙金红,侯宁宁,孙红喜）,药剂科（周学伟）；261042　潍坊医学院病理学教研室（李文通）
Author(s):
Hui Zongguang*; Zhou Xuewei; Li Wentong; Sun Jinhong; Hou Ningning; Sun Hongxi.
*Department of Endocrinology,Affiliated Hospital of Weifang Medical University,Weifang 261041,China
关键词:
β3肾上腺素能受体; 基因多态性; 糖尿病足; 胰岛素抵抗
Keywords:
Beta-3 adrenergic receptor; Gene polymorphism; Diabetic foot; Insulin resistance
DOI:
10.3760/cma.j.issn.1673-4157.2014.02.019
摘要:
目的　探讨中国人群β3肾上腺素能受体（β3-AR）基因 Trp64Arg突变与糖尿病足的关系。方法　应用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测110例2型糖尿病患者（T2DM组）[其中糖尿病足患者58例（DFU组）,单纯2型糖尿病患者52例（N-DFU组）]和42例正常人（正常对照组）β3-AR基因的Trp64Arg突变,比较各组的基因型（TT型,TA型或AA型）和基因频率（T或A）；并进行人体测量学和代谢指标的检测。结果　（1）β3-AR基因Trp64Arg基因型频率符合Hardy-Weinberg平衡定律,T2DM组β3-AR基因64位Arg突变患者,其体重指数和稳态模型评估-胰岛素抵抗指数（HOMA-IR）显著高于无突变组（P =0.007 2,P =0.038 6）。（2）在T2DM组,Arg64携带者血高密度脂蛋白-胆固醇水平明显低于非携带者（P =0.047 5）。（3）DFU组Arg64等位基因频率明显高于N-DFU组（?字2= 5.147,P＜0.05）,而T2DM组与正常对照组β3-AR基因型及等位基因频率无统计学意义（?字2= 0.067, P ＞0.05）。结论　β3-AR基因多态性与糖尿病足存在相关性,Arg64等位基因可能是糖尿病足的危险因素之一。
Abstract:
Objective　To explore the relationship between Trp64Arg polymorphism of the β3-adrenergic receptor（β3-AR） gene and diabetic foot in Chinese subjects. Methods　The mutational genotype of Trp64Arg in β3-AR gene was measured by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） in 110 patients with type 2 diabetes mellitus（T2DM group） [（58 with diabetic foot ulcer （DFU group） and 52 without （N-DFU group）] and 42 normal healthy subjects （normal control group）. The genotype（TT,TA or AA） and allele frequencies（T or A） were compared among groups. The anthro-pometry and metabolic indices were also compared between groups. Results　（1） No significant deviation from Hardy-Weinberg equilibrium was found in the β3-AR Trp64Arg genotype frequency. In T2DM group,body mass index and homeostasis model assessment for insulin resistance index（HOMA-IR） in Arg allele were significantly higher than those without mutations allele （P =0.007 2, P =0.038 6）. （2） In T2DM group, Arg64 carriers had lower level of high density lipoprotein-cholesterol than those without this mutation（P =0.047 5）.（3）The frequency of Arg64 allele in DFU group was greatly higher than that of N-DFU group（?字2= 5.147,P＜0.05）,while no difference in β3-AR gene genotype and allele frequency was observed between T2DM group and normal control group（?字2=0.067,P ＞0.05）. Conclusion　β3-AR gene polymorphism is associated with diabetic foot. The Arg64 allele may be one of the risk factors of diabetic foot.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目：山东省高等学校科技计划基金项目（J12LK05）
更新日期/Last Update: 2014-03-20