[1]马金刚 魏红丽 尚恒.Graves病患者外周血T细胞亚群与甲状腺 功能及甲状腺自身抗体间的相关性分析[J].国际内分泌代谢杂志,2020,40(01):21-26.[doi:10.3760/cma.j.issn.1673-4157.2020.01.005]
　Ma Jingang,Wei Hongli,Shang Heng.Correlation between peripheral blood T cell subsets and thyroid function and thyroid autoantibodies in patients with Graves' disease[J].International Journal of Endocrinology and Metabolism,2020,40(01):21-26.[doi:10.3760/cma.j.issn.1673-4157.2020.01.005]
点击复制

Graves病患者外周血T细胞亚群与甲状腺 功能及甲状腺自身抗体间的相关性分析()
分享到：

《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
40
期数:
2020年01期
页码:
21-26
栏目:
论著
出版日期:
2020-01-20

文章信息/Info

Title:
Correlation between peripheral blood T cell subsets and thyroid function and thyroid autoantibodies in patients with Graves' disease
作者:
马金刚 魏红丽 尚恒
沧州市人民医院体检中心 061000
Author(s):
Ma Jingang;  Wei Hongli;  Shang Heng
Physical Examination Center of Cangzhou People's Hospital, Cangzhou 061000, China
关键词:
Graves病;  辅助性T细胞;  调节性T细胞;  甲状腺自身抗体
Keywords:
Graves' disease;  Helper T cells;  Regulatory T cells;  Thyroid autoantibodies
DOI:
10.3760/cma.j.issn.1673-4157.2020.01.005
摘要:
目的 分析Graves病患者外周血T细胞亚群之间的相关性以及T细胞亚群与甲状腺功能及甲状腺自身抗体之间的相关性。方法 选取沧州市人民医院2017年12月至2018年6月收治的初发Graves病患者65例作为GD组,同期来我院的健康体检者50名作为对照组,采集研究对象空腹外周静脉血,电化学发光法测定血清FT3、FT4和促甲状腺激素(TSH)水平,免疫化学发光法测定甲状腺球蛋白抗体(TGAb)、甲状腺过氧化物酶抗体(TPOAb)和促甲状腺激素受体抗体(TRAb)水平,流式细胞仪测定辅助性T细胞(Th)1、Th2、Th17和调节性T细胞(Treg细胞)占CD4+T细胞的比例,并行T细胞亚群之间的相关性分析以及T细胞亚群与甲状腺功能及甲状腺自身抗体之间的相关性分析。结果 GD组Th1、Th2和Th17占CD4+T细胞的比例均高于对照组(t=14.10、4.63、17.71,P均<0.01),Treg细胞占CD4+T细胞的比例低于对照组(t=-11.25,P<0.01); GD组Th1占CD4+T细胞的比例与Th17占CD4+T细胞的比例呈正相关(r=0.342,P=0.005),其余T细胞亚群之间未发现显著相关性(P均>0.05)。GD组Th1占CD4+T细胞的比例与FT4、TPOAb水平呈正相关(r=0.405、0.568,P均<0.01); Th2占CD4+T细胞的比例与TRAb水平呈正相关(r=0.383,P=0.002); Th17占CD4+T细胞的比例与FT4、TPOAb水平呈正相关(r=0.312、0.330,P均<0.01); Treg细胞占CD4+T细胞的比例与FT3、FT4、TGAb、TPOAb、TRAb之间未发现显著相关性(P均>0.05)。结论 在Graves病患者外周血中,Th1和Th17占CD4+T细胞的比例与FT4和TPOAb水平呈正相关,而Th2占CD4+T细胞的比例与TRAb水平呈正相关。
Abstract:
Objective To analyze the correlation between T cell subsets of peripheral blood, the correlation between T cell subsets and thyroid function, as well as the correlation between T cell subsets and thyroid autoantibodies in patients with Graves' disease(GD).Methods A total of 65 patients with newly diagnosed GD who were admitted to the Cangzhou People's Hospital from December 2017 to June 2018 were enrolled in the GD group. Another 50 healthy subjects from our hospital in the same period were enrolled in the control group. Fasting peripheral venous blood were collected from subjects. The levels of FT3, FT4 and thyroid stimulating hormone(TSH)were determined by electrochemiluminescence method. The levels of serum thyroglobulin antibody(TGAb), thyroid peroxidase autoantibodies(TPOAb)and thyrotropin receptor antibody(TRAb)were determined by immunochemiluminescence method. The percentage of helper T cells(Th)1, Th2, Th17 and regulatory T cells(Treg cells)in CD4+T cells were determined by flow cytometry. The correlation between T cell subsets, the correlation between T cell subsets and thyroid function, as well as the correlation between T cell subsets and thyroid autoantibodies were analyzed.Results The percentage of Th1, Th2 and Th17 in CD4+T cells in GD group were higher than those in control group(t=14.10, 4.63, 17.71, all P<0.01), and the percentage of Treg cells in CD4+T cells was lower than that in control group(t=-11.25, P<0.01). The percentage of Th1 in CD4+T cells was positively correlated with the percentage of Th17 in CD4+T cells(r=0.342, P=0.005), and there was no significant correlation between the other T cell subsets in GD group. In GD group, the proportion of Th1 in CD4+T cells was positively correlated with the levels of FT4 and TPOAb(r=0.405, 0.568, P<0.01). The proportion of Th2 in CD4+T cells was positively correlated with TRAb level(r=0.383, P=0.002). The proportion of Th17 in CD4+T cells was positively correlated with the levels of FT4 and TPOAb(r=0.312, 0.330, P<0.01). There was no significant correlation between the ratio of Treg cells to CD4+T cells and FT3, FT4, TGAb, TPOAb, TRAb(all P>0.05).Conclusion In peripheral blood of patients with GD, the percentage of Th1, Th17 in CD4+T cells is positively correlated with the levels of FT4 and TPOAb, while the percentage of Th2 in CD4+T cells is positively correlated with the level of TRAb.

参考文献/References:

[1] Ramos-Leví AM,Marazuela M.Pathogenesis of thyroid autoimmune disease:the role of cellular mechanisms[J].Endocrinol Nutr,2016,63(8):421-429.DOI:10.1016/j.endonu.2016.04.003.
[2] Rydzewska M,Jaromin M,Pasierowska IE,et al.Role of the T and B lymphocytes in pathogenesis of autoimmune thyroid diseases[J].Thyroid Res,2018,11:2.DOI:10.1186/s13044-018-0046-9.
[3] Park H,Li Z,Yang XO,et al. A distinct lineage of CD4+T cells regulates tissue inflammation by producing interleukin 17[J].Nat Immunol,2005,6(11):1133-1141.DOI:10.1038/ni1261.
[4] Phenekos C,Vryonidou A,Gritzapis AD,et al.Th1 and Th2 serum cytokine profiles characterize patients with Hashimoto's thyroiditis(Th1)and Graves' disease(Th2)[J].Neuroimmunomodulation,2004,11(4):209-213. DOI:10.1159/000078438.
[5] Klatka M,Grywalska E,Partyka M,et al.Th17 and Treg cells in adolescents with Graves' disease. Impact of treatment with methimazole on these cell subsets[J].Autoimmunity,2014,47(3):201-211.DOI:10.3109/08916934.2013.879862.
[6] 王建国,史春云,丑广程,等.Graves病患者血清TLR4、NF-κB和Treg/Th17 相关细胞因子表达变化及意义[J]. 中国现代医学杂志,2017, 27(24):58-61.DOI:10.3969/j.issn.1005-8982.2017.24.012.
[7] Shen J,Li Z,Li W,et al.Th1,Th2, and Th17 cytokine involvement in thyroid associated ophthalmopathy[J].Dis Markers,2015,2015:609593.DOI:10.1155/2015/609593.
[8] Rydzewska M,Jaromin M,Pasierowska IE,et al.Role of the T and B lymphocytes in pathogenesis of autoimmune thyroid diseases[J].Thyroid Res,2018,11:2.DOI:10.1186/s13044-018-0046-9.
[9] Bossowski A,Moniuszko M,Id kowska E,et al.Decreased proportions of CD4+IL17+/CD4+CD25+ CD127- and CD4+IL17+/CD4+CD25+CD127- FoxP3+ T cells in children with autoimmune thyroid diseases[J].Autoimmunity,2016,49(5):320-328.DOI:10.1080/08916934.2016.1183654.
[10] Kuang M,Wang S,Wu M,et al.Expression of IFNalpha-inducible genes and modulation of HLA-DR and thyroid stimulating hormone receptors in Graves' disease[J].Mol Cell Endocrinol,2010,319(1-2):23-29.DOI:10.1016/j.mce.2009.12.006.
[11] Tsang J,Lu L,Jiang S.TH17 cells in organ transplantation rejection and tolerance[M]//TH17 Cells in Health and Disease.Springer New York, 2011: 319-339.
[12] 张敏,罗嘉,段雨函.TGAb、TMAb、TPOAb水平与Graves病治疗后甲状腺功能减退发生率的相关性研究[J].检验医学与临床,2017,14(6):816-818.DOI:10.3969/j.issn.1672-9455.2017.06.027.

相似文献/References:

[1]陈晓铭 胡卓清 李潍 刘美莲 吴美芬 方烁 武革.CD40-1C/T多态性（rs1883832）与粤西汉族人Graves病的关系[J].国际内分泌代谢杂志,2015,(01):11.[doi:10.3760/cma.j.issn.1673-4157.2015.01.003]
　Chen Xiaoming,Hu Zhuoqing,Li Wei,et al.Relationship between CD40-1C/T polymorphism（rs1883832）and Graves′ disease of Han population in western region of Guangdong province[J].International Journal of Endocrinology and Metabolism,2015,(01):11.[doi:10.3760/cma.j.issn.1673-4157.2015.01.003]
[2]赵永强,田德增,魏晓华,等.不同治疗方法对Graves病患者外周血淋巴细胞亚群的影响[J].国际内分泌代谢杂志,2015,(04):234.[doi:10.3760/cma.j.issn.1673-4157.2015.04.005]
　Zhao Yongqiang*,Tian Dezeng,Wei Xiaohua,et al.Effects of different therapeutic methods on peripheral blood lymphocyte subgroups in patients with Graves' disease[J].International Journal of Endocrinology and Metabolism,2015,(01):234.[doi:10.3760/cma.j.issn.1673-4157.2015.04.005]
[3]瞿茹怡,李圣贤,陆咏,等.2q33区基因多态性与Graves病发病风险的研究[J].国际内分泌代谢杂志,2014,(02):77.[doi:10.3760/cma.j.issn.1673-4157.2014.02.002]
　Qu Ruyi*,Li Shengxian,Lu Yong,et al.Association between 2q33 gene polymorphism and Graves' disease[J].International Journal of Endocrinology and Metabolism,2014,(01):77.[doi:10.3760/cma.j.issn.1673-4157.2014.02.002]
[4]朱萍,卫红艳,王坤玲,等.Graves病甲状腺功能亢进症性肝损害的 相关因素分析[J].国际内分泌代谢杂志,2017,37(03):164.[doi:10.3760/cma.j.issn.1673-4157.2017.03.005]
　Zhu Ping,Wei Hongyan,Wang Kunling,et al.Related factors of hyperthyroidism-related liver dysfunction in Graves' disease[J].International Journal of Endocrinology and Metabolism,2017,37(01):164.[doi:10.3760/cma.j.issn.1673-4157.2017.03.005]
[5]陈莹 倪文婧 陈国芳 徐书杭.基于复发危险因素探讨Graves病治疗的新策略[J].国际内分泌代谢杂志,2022,42(04):260.[doi:10.3760/cma.j.cn121383-20210412-04031]
　Chen Ying,Ni Wenjing,Chen Guofang,et al.New strategies for the treatment of Graves' disease based on risk factors of relapse[J].International Journal of Endocrinology and Metabolism,2022,42(01):260.[doi:10.3760/cma.j.cn121383-20210412-04031]
[6]陈莹 徐书杭.2022年欧洲甲状腺学会《儿童Graves病管理指南》解读[J].国际内分泌代谢杂志,2023,43(05):449.[doi:10.3760/cma.j.cn121383-20220629-06064]
　Chen Ying,Xu Shuhang.Interpretation of 2022 European Thyroid Association Guideline for the management of pediatric Graves' disease[J].International Journal of Endocrinology and Metabolism,2023,43(01):449.[doi:10.3760/cma.j.cn121383-20220629-06064]

备注/Memo

备注/Memo:
通信作者:尚恒,Email:379661596@qq.com
基金项目:沧州市科技计划项目(172302048)
Corresponding author: Shang Heng, Email:379661596@qq.com
Fund program:Cangzhou Science and Technology Plan Project(172302048)
更新日期/Last Update: 2020-01-20