[1]赵紫婷,孟颖飞,徐坤,等.邻苯二甲酸二(2-乙基己)酯对胰岛MIN6 β细胞毒性作用的研究[J].国际内分泌代谢杂志,2017,37(03):145-148，167.[doi:10.3760/cma.j.issn.1673-4157.2017.03.001]
　Zhao Ziting,Meng Yingfei,Xu Kun,et al.Cytotoxic effects of di-(2-ethylhexyl)phthalate on MIN6 islet β cells[J].International Journal of Endocrinology and Metabolism,2017,37(03):145-148，167.[doi:10.3760/cma.j.issn.1673-4157.2017.03.001]
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邻苯二甲酸二(2-乙基己)酯对胰岛MIN6 β细胞毒性作用的研究()
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《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
37
期数:
2017年03期
页码:
145-148，167
栏目:
论著
出版日期:
2017-05-20

文章信息/Info

Title:
Cytotoxic effects of di-(2-ethylhexyl)phthalate on MIN6 islet β cells
作者:
赵紫婷; 孟颖飞; 徐坤; 何继瑞
730000 兰州大学第二医院内分泌代谢3科
Author(s):
Zhao Ziting;  Meng Yingfei;  Xu Kun;  He Jirui.
Endocrinology and Metabolism Center 3 of the Second Hospital, Lanzhou University, Lanzhou 730000, China
关键词:
邻苯二甲酸二(2-乙基己)酯;  MIN6 β细胞;  细胞凋亡;  细胞周期
Keywords:
Di-(2-ethylhexyl)phthalate;  MIN6 β cells;  Apoptosis;  Cell cycle
DOI:
10.3760/cma.j.issn.1673-4157.2017.03.001
摘要:
目的 观察邻苯二甲酸二(2-乙基己)酯(DEHP)对胰岛MIN6 β细胞生长活力、细胞周期及细胞凋亡的影响。方法 选用小鼠胰岛MIN6 β细胞作为研究对象,并将其分为DEHP组和对照组,DEHP组分别用0.25、0.5、1、2 mmol/L DEHP作用16、48、72 h。对照组用0.1%二甲基亚砜(DMSO)作用16、48、72 h。通过MTT实验检测细胞生长活力,流式细胞技术测定细胞凋亡率及细胞周期。结果 与对照组相比,在16、48及72 h随着DEHP浓度的增加,MIN6 β细胞生长活力受到抑制,细胞存活率明显下降(F=112.25、91.06、589.25,P均<0.01); 0.25、0.5、1 mmol/L DEHP组 MIN6 β细胞凋亡率明显升高[(5.00±0.22)%、(8.33±0.09)%、(19.62±0.44)%,F=3 006.09,P<0.01]; 0.5、1 mmol/LDEHP组受到MIN6 β细胞周期进程受到干扰,G0/G1期细胞比例[(68.50±0.89)%、(73.63±0.96)%,F=242.46,P<0.01]明显升高,S期细胞[(17.57±1.15)%、(14.40%±0.91)%,F=49.28,P<0.01]与G2/M期[(13.93±0.42)%、(11.97±1.85)%,F=72.70,P<0.01]比例明显降低。结论 DEHP可抑制胰岛MIN6 β细胞增殖、诱导细胞凋亡、导致细胞周期停滞在细胞间期。
Abstract:
Objective To investigate the cytotoxic effects of di-(2-ethylhexyl)phthalate(DEHP)on viability, proliferation and apoptosis of MIN6 islet β cells.Methods Murine MIN6 islet β cells were used in this study and were divided into DEHP group and control group. In DEHP group, MIN6 β cells were exposed to 0.25, 0.5, 1 or 2 mmol/L DEHP for 16, 48 or 72 hours. Cells in control group were exposed to dimethylsulfoxide(0.1%)for 16, 48 or 72 hours. Cell viability was determined by MTT assay. Apoptosis rate and cell cycle were determined using flow cytometric assay.Results Compared with control group, treatment of MIN6 β cells with DEHP inhibited cell viability and decreased cell survival rate in a consentration-dependent manner at 16, 48, 72 h(F=112.25, 91.06, 589.25, all P<0.01); treatment of MIN6 β cells with 0.25, 0.5 or 1 mmol/L DEHP led to a marked increase in apoptosis rate [(5.00±0.22)%,(8.33±0.09)%,(19.62±0.44)%, F=3 006.09,P<0.01]; treatment of MIN6 β cells with 0.5 or 1 mmol/L DEHP significantly disrupted cell cycle, increased the percentage of G0/G1 cells [(68.50±0.89)%,(73.63±0.96)%, F=242.46,P<0.01] and decreased the percentage of cells entering S phase [(17.57±1.15)%,(14.40%±0.91)%, F=49.28, P<0.01] and G2/M phase[(13.93±0.42)%,(11.97±1.85)%, F=72.70, P<0.01].Conclusion DEHP inhibits cell proliferation, induces apoptosis and arrests cell cycle in interphase in MIN6 islet β cells.

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备注/Memo

备注/Memo:
基金项目:甘肃省自然科学基金资助项目(1308RJZA246) 通信作者:何继瑞,Email: hjrlzys63@163.com
更新日期/Last Update: 2017-05-20