[1]李兴佳,郑全喜,陈国芳,等.七叶皂苷钠抑制人甲状腺未分化癌HTh74 细胞株增殖作用的研究[J].国际内分泌代谢杂志,2016,36(02):116-120.[doi:10.3760/cma.j.issn.1673-4157.2016.02.010]
　Li Xingjia,Zheng Quanxi,Chen Guofang,et al.Inhibitory effects of sodium aescinate on cell proliferation in human anaplastic thyroid carcinoma cell HTh74[J].International Journal of Endocrinology and Metabolism,2016,36(02):116-120.[doi:10.3760/cma.j.issn.1673-4157.2016.02.010]
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七叶皂苷钠抑制人甲状腺未分化癌HTh74 细胞株增殖作用的研究()
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《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
36
期数:
2016年02期
页码:
116-120
栏目:
论著
出版日期:
2016-03-20

文章信息/Info

Title:
Inhibitory effects of sodium aescinate on cell proliferation in human anaplastic thyroid carcinoma cell HTh74
作者:
李兴佳; 郑全喜; 陈国芳; 茅晓东; 刘超
210028 南京中医药大学附属中西医结合医院,江苏省中医药研究院,国家中医药管理局瘿病证治重 点研究室
Author(s):
Li Xingjia;  Zheng Quanxi;  Chen Guofang;  Mao Xiaodong; Liu Chao.
Jiangsu Province Hospital on Integration of Chinese and Western Medicine Affiliated to Nanjing University of Traditional Chinese Medicine, Jiangsu Provincial Academy of Chinese Medicine,Key Laboratory of TCM Syndrome&Treatment of Yingbing of State Adm
关键词:
甲状腺未分化癌;  HTh74;  七叶皂苷钠;  细胞周期;  细胞凋亡
Keywords:
Anaplastic thyroid cancer;  HTh74;  Sodiun aescinate;  Cell cycle;  Apoptosis
DOI:
10.3760/cma.j.issn.1673-4157.2016.02.010
摘要:
目的 研究七叶皂苷钠对人甲状腺未分化癌HTh74细胞株增殖的潜在抑制作用及其分子机制。方法 以不同浓度的七叶皂苷钠(0,20,40,60,80 μmol/L)处理HTh74细胞48 h后,观察细胞形态变化; 以不同浓度的七叶皂苷钠(0,20,40,60,80 μmol/L)处理HTh74细胞24 h、48 h和72 h后,噻唑蓝比色(MTT)法检测七叶皂苷钠对HTh74细胞株增殖的抑制效应并计算细胞增殖率及半数抑制率(IC50); 以不同浓度的七叶皂苷钠(0,5,10,15,20,25 μmol/L)处理HTh74细胞48 h后,Western印迹检测七叶皂苷钠引起的蛋白激酶B(Akt)、磷酸化Akt(p-Akt)以及细胞周期相关蛋白的表达。结果 七叶皂苷钠作用后,细胞发生明显皱缩,且作用浓度越高细胞皱缩越明显; MTT法结果显示,七叶皂苷钠对HTh74细胞有显著的生长抑制作用,呈现典型的时间与剂量依赖性,80 μmol/L七叶皂苷钠作用24 h,对细胞的生长抑制作用有统计学意义(F=111.4,P<0.01),而40 μmol/L作用48 h及72 h时, 细胞生长均受到抑制(F=543.6,722.1,P<0.01),并且48 h的IC50为69.4 μmol/L,72 h的IC50为32.5 μmol/L; 七叶皂苷钠作用48 h后Akt、p-Akt和细胞周期蛋白依赖性激酶(CDK)2的表达显著降低,并呈现剂量依赖效应。与未经处理的细胞相比,低浓度(5 μmol/L和10 μmol/L)七叶皂苷钠处理时Akt和p-Akt的表达水平已开始降低(F=613.9,P<0.05),而从15 μmol/L开始,CDK2表达的降低有统计学意义(F=208.9,208.3,P<0.05)。CDK1、CDK6、细胞周期蛋白D以及细胞周期蛋白E的表达在各处理组间差异无统计学意义(P均>0.05)。结论 七叶皂苷钠可抑制人甲状腺未分化癌HTh74细胞的增殖,可能是通过调节Akt、p-Akt以及CDK2等蛋白的表达实现的。
Abstract:
Objective To investigate the potent inhibitory effects of sodium aescinate on human anaplastic thyroid cancer cell line HTh74 and its molecular mechanism. Methods Different concentrations of sodium aescinate(0, 20, 40, 60, 80 μmol/L)were used to treat HTh74 cells for 48 h, and the morphological changes of cells were observed. After treated with different concentrations of sodium aescinate(0, 20, 40, 60, 80 μmol/L), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide(MTT)assay was used to detect the inhibitory effects of sodium aescinate on proliferation of HTh74 cells and cell proliferation rate, 50% inhibitory concentration(IC50)were calculated. After treated with different concentrations of sodium aescinate(0, 5, 10, 15, 20, 25 μmol/L), Western blotting was performed to detect the expression of protein kinase B(Akt), phosphorylated Akt(p-Akt)and cell cycle-related proteins. Results After treated with sodium aescinate, cells shrinked significantly in a dose dependent manner. MTT assay showed that sodium aescinate had obvious inhibitory effect on HTh74 cell growth in a time and dose dependent manner, when treated with 80 μmol/L sodium aescinate for 24 h, the inhibitory effects on the cell growth had statistical significance(F=111.4,P<0.01), while treated with 40 μmol/L for 48 h and 72 h, the cell growth was inhibited(F=543.6,722.1,all P<0.01), and the IC50 was 69.4 μmol/L at 48 h and 32.5 μmol/L at 72 h. The expression of Akt, p-Akt and cyclin-dependent kinase 2(CDK2)significantly decreased in a dose dependent manner after treated with sodium aescinate for 48 h. Compared with untreated cells, expression of Akt and p-Akt began to decrease when treated with low concentration(5 μmol/L and 10 μmol/L)of sodium aescinate(F=613.9,P<0.05),while expression of CDK2 decreased when treated with 15 μmol/L sodium aescinate(F=208.9, 208.3, all P<0.05). But the expression of CDK1,CDK6,cyclin D and cyclin E were not different among the groups(all P>0.05). Conclusion Sodium aescinate can inhibit the proliferation of HTh74 cells, and the molecular mechanisms may be mediated by regulation of Akt, p-Akt and CDK2.

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更新日期/Last Update: 2016-03-20