[1]褚月颉,王鹏华,李代清,等.糖尿病足患者创面肉芽组织中Angiomotin表达的探讨[J].国际内分泌代谢杂志,2015,(04):226-229.[doi:10.3760/cma.j.issn.1673-4157.2015.04.003]
　Chu Yuejie*,Wang Penghua,Li Daiqing,et al.Angiomotin expression in wound granulation tissues from patients with diabetic foot[J].International Journal of Endocrinology and Metabolism,2015,(04):226-229.[doi:10.3760/cma.j.issn.1673-4157.2015.04.003]
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糖尿病足患者创面肉芽组织中Angiomotin表达的探讨()
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《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
期数:
2015年04
页码:
226-229
栏目:
论著
出版日期:
2015-07-20

文章信息/Info

Title:
Angiomotin expression in wound granulation tissues from patients with diabetic foot
作者:
褚月颉; 王鹏华; 李代清; 于德民
300120　天津市中医药研究院附属医院糖尿病科（褚月颉）；300070　天津医科大学代谢病医院，卫生部激素与发育重点实验室（王鹏华，李代清，于德民） 　　
Author(s):
Chu Yuejie*; Wang Penghua; Li Daiqing; Yu Demin.
*Department of Diabetes Mellitus，Tianjin Chinese Medicine Research Institute Affiliated Hospital，Tianjin 300120，China Corresponding author：Wang Penghua，Email:wph200000@163.com
关键词:
Angiomotin; 高糖; 缺血; 糖尿病足; 溃疡预后
Keywords:
Angiomotin; High glucose; Ischemia; Diabetic foot; Ulcer prognosis
DOI:
10.3760/cma.j.issn.1673-4157.2015.04.003
摘要:
目的　测定Angiomotin （Amot）蛋白在糖尿病足溃疡患者创面肉芽组织中的表达，探讨高糖及缺血对Amot表达的影响及其与溃疡预后的关系。方法　选取天津医科大学代谢病医院糖尿病足病科住院的糖尿病足溃疡患者，均符合Wagner分级2~4级，共28例。根据溃疡侧踝肱指数（ABI）及HbA1c将糖尿病足溃疡患者分为4组：A组：ABI≥0.7，HbA1c＜9%；B组：ABI≥0.7，HbA1c≥9%；C组：ABI＜0.7，HbA1c＜9%；D组：ABI＜0.7，HbA1c≥9%，每组7例。以同时期足部烧伤患者为非糖尿病、非缺血对照组。取各组患者足溃疡创面肉芽组织，应用Western印迹法测定Amot蛋白的表达，每例糖尿病足患者进行Wagner分级诊断并随访溃疡的预后情况。结果　比较4组糖尿病足患者年龄、性别、糖尿病病程、并发症、肾功能、血脂、超敏C反应蛋白，差异均无统计学意义（P均＞0.05）。与对照组相比，p80-Amot在B组和D组表达明显减少（P均＜0.05）。与A组相比，p80-Amot在B组和D组表达明显减少（P均＜0.05）。p130-Amot在各组间的表达差异无统计学意义（P ＞0.05）。各组患者足溃疡的预后情况没有明显差异（P ＞0.05）。结论　高血糖和缺血都会造成糖尿病足患者创面肉芽组织中Amot蛋白表达下降，其中高血糖的作用更显著。Amot蛋白表达与溃疡预后无明显相关。
Abstract:
Objective　To determine the expression of Angiomotin（Amot） in wound granulation tissues from patients with diabetic foot，and investigate the effects of high glucose and ischemia on the expression of Amot and the relationship between Amot expression and ulcer prognosis. Methods　Twenty-eight diabetic inpatients with foot ulcers（Wagner's 2-4 grade） from the Department of Diabetic Foot in the Metabolic Diseases Hospital of Tianjin Medical University，were divided into four groups according to their ankle brachial index（ABI） and HbA1c：group A：ABI≥0.7，HbA1c＜9%；group B：ABI≥0.7，HbA1c≥9%；group C：ABI＜0.7，HbA1c＜9% and group D：ABI＜0.7， HbA1c≥9%，with 7 cases in each group. Patients with foot burns at the same time were included as non-diabetic and non-ischemic control group. Wound granulation tissues were sampled from these patients. Western blot was used to determine the expression of Amot. Wagner's grade was assessed in each patient with diabetic foot and the patients were followed up for the evaluation of ulcer prognosis. Results　The age，gender，course of diabetes，complications，renal function，blood lipid，and sensitive C reactive protein were compared，but no significant difference were found between four diabetic foot groups（all P ＞0.05）. Compared with control group，expression of p80-Amot in group B and group D were significantly reduced （all P ＜ 0.05）. Compared with group A，expression of p80-Amot in group B and group D were also significantly reduced （all P ＜ 0.05）. There was no significant difference in expression of p130-Amot between groups （P ＞0.05）. There was also no significant difference in ulcer prognosis between groups（P ＞0.05）. Conclusions　High glucose and ischemia decrease the expression of Amot in wound granulation tissues in patients with diabetic foot，and high glucose has a more potent effect. There is no significant relationship between Amot expression and ulcer prognosis.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目：天津市自然科学基金重点项目（10JCZDJC19800）；天津市卫生局科技基金（09KZ87） 通信作者：王鹏华，Email:wph200000@163.com
更新日期/Last Update: 2015-07-20