[1]刘司漩,刘云峰,尹建红,等.小檗碱对脂多糖诱导的THP-1细胞相关炎性反应因子的影响[J].国际内分泌代谢杂志,2014,(04):233-236.[doi:10.3760/cma.j.issn.1673-4157.2014.04.005]
 Liu Sixuan*,Liu Yunfeng,Yin Jianhong,et al.Effects of berberine on inflammatory cytokines induced by lipopolysaccharide in THP-1 cells[J].International Journal of Endocrinology and Metabolism,2014,(04):233-236.[doi:10.3760/cma.j.issn.1673-4157.2014.04.005]
点击复制

小檗碱对脂多糖诱导的THP-1细胞相关炎性反应因子的影响()
分享到:

《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
期数:
2014年04期
页码:
233-236
栏目:
论著
出版日期:
2014-08-31

文章信息/Info

Title:
Effects of berberine on inflammatory cytokines induced by lipopolysaccharide in THP-1 cells
作者:
刘司漩刘云峰尹建红章毅许林鑫杨静
作者单位:030001 太原,山西医科大学第一医院内分泌科(刘司漩,刘云峰,尹建红,许林鑫,杨静);030001 太原,山西医科大学基础医学院药理教研室(章毅)   
Author(s):
Liu Sixuan*Liu YunfengYin JianhongZhang YiXu LinxinYang Jing.
*Department of Endocrinology,The First Hospital of Shanxi Medical University,Shanxi 030001,China Corresponding author:Yang Jing,Email:yangjlm@126.com
关键词:
小檗碱脂多糖THP-1细胞炎症因子
Keywords:
BerberineTHP-1 cellsLipopolysaccharideInflammatory cytokines
DOI:
10.3760/cma.j.issn.1673-4157.2014.04.005
摘要:
目的 通过观察小檗碱对脂多糖诱导的人单核细胞(THP-1)相关炎性反应因子表达的影响,探讨小檗碱的直接抗炎作用。方法 小檗碱毒性作用分析:体外培养THP-1细胞,分为对照组和不同浓度小檗碱组(小檗碱5,10,20,50 ?滋mol/L),分别孵育6 h、24 h、48 h,收集培养上清,应用乳酸脱氢酶(LDH)微量释放法检测不同浓度小檗碱对THP-1细胞的毒性作用。小檗碱对脂多糖诱导的THP-1细胞炎性因子的影响:分为对照组、脂多糖组(1 ?滋g/ml)、不同浓度小檗碱+脂多糖组(小檗碱5、10、20 ?滋mol/L +1 ?滋g/ml 脂多糖),分别孵育6 h、24 h、48 h,收集培养上清,应用酶联免疫吸附试验(ELISA)检测白细胞介素(IL)-1β、IL-6、IL-8和肿瘤坏死因子(TNF)-?琢的水平。结果 当小檗碱浓度<20 ?滋mol/L时,THP-1细胞经小檗碱干预6 h、24 h、48 h之后,细胞存活率在90%以上,与对照组相比差异无统计学意义(P均>0.05)。小檗碱呈剂量依赖性的降低脂多糖诱导的THP-1细胞培养上清IL-1β、IL-6、IL-8和TNF-?琢水平。6 h时,20 ?滋mol/L 小檗碱+脂多糖组IL-1β、IL-8、TNF-?琢水平与脂多糖组相比显著下降(P均<0.05);24 h时,20 ?滋mol/L 小檗碱+脂多糖组IL-8和TNF-?琢水平与脂多糖组相比均显著下降(P均<0.05);48 h时,5 ?滋mol/L 小檗碱+脂多糖组TNF-?琢水平显著低于脂多糖组(F =92.625,P <0.05); 10 ?滋mol/L小檗碱+脂多糖组IL-1β、IL-6和TNF-?琢水平亦显著低于脂多糖组(P均<0.05);20 ?滋mol/L 小檗碱+脂多糖组IL-1β、IL-6、IL-8和TNF-?琢水平亦显著低于脂多糖组(P均<0.05)。结论 小檗碱可显著降低脂多糖诱导的THP-1细胞分泌炎性反应因子,且具有一定的剂量-效应关系。
Abstract:
Objective To observe the effects of berberine(BBR) on expression of inflammatory cytokines in THP-1 cells induced by lipopolysaccharide(LPS) ,and investigate the anti-inflammatory effects of BBR. Methods For analysing the toxicity of BBR on THP-1 cells, THP-1 cells were divided into control group,and different concentrations of BBR groups(BBR 5,10, 20,50 ?滋mol/L) . After incubation for 6, 24 and 48 hours,lactate dehydrogenase(LDH) released from THP-1 cells was used to assay the cytotoxicity of BBR. For analysis of the effects of BBR on inflammatory cytokines induced by LPS in THP-1 cells,THP-1 cells were divided into control group,LPS group(1 ?滋g/mL LPS),and different concentrations of BBR with LPS groups (BBR 5,10 and 20 ?滋mol/L + 1 ?滋g/mL LPS). After 6,24 or 48 hours of incubation,the concentrations of inter-leukin(IL)-1β, IL-6,IL-8 and tumor necrosis factor(TNF)-?琢 in the culture medium were measured by enzyme-linked immunosorbent assay(ELISA). Results The survival rates of THP-1 cells were all over 90% after treated with BBR lower than 20 ?滋mol/L for 6,24 and 48 hours. BBR decreased the release of IL-1β,IL-6,IL-8 and TNF-?琢 from THP-1 cells in a dose-dependent manner. After 6 hours,20 ?滋mol/L of BBR decreased the secretion of IL-1β,IL- 8 and TNF-?琢 significantly compared with LPS group (P < 0.05). After 24 hours,the secretion of IL-8 and TNF-?琢 was decreased significantly in 20 ?滋mol/L of BBR + LPS group compared with those in LPS group (P < 0.05). After 48 hours,the secretion of TNF-?琢 was decreased signifi-cantly(F =92.625,P < 0.05) in 5 ?滋mol/L BBR + LPS group,the secrection of IL-1β、 IL- 6 and TNF-?琢 were decreased (all P < 0.05) in 10 ?滋mol/L BBR + LPS group, and the secretion of IL-1β,IL - 6 and TNF-?琢 were decreased in 20 ?滋mol/L BBR + LPS group compared with those in LPS group (all P < 0.05). Conclusion BBR can decrease the secretion of inflammatory cytokines in THP-1 cells induced by LPS in a dose-dependent manner.

参考文献/References:

 [1] Lontchi-Yimagou E,Sobngwi E,Matsha TE,et al.Diabetes mellitus and inflammation[J].Curr Diab Rep,2013,13 (3):435-444.  
[2] Chen C,Zhang Y,Huang C.Berberine inhibits PTP1B activity and mimics insulin action[J].Biochem Biophys Res Commun,2010,397 (3):543-547.  
[3] Yang J,Yin J,Gao H,et al.Berberine improves insulin sensitivity by inhibiting fat store and adjusting adipokines profile in human preadipocytes and metabolic syndrome patients[J].Evid Based Complement Alternat Med,2012,2012:363845.  
[4] 范梅琳,刘云峰,章毅,等.二甲双胍对脂多糖诱导的THP-1细胞相关炎症因子及凋亡的影响[J].中华内分泌代谢杂志,2013,29(9):801-803.  
[5] Sell H,Habich C,Eckel J.Adaptive immunity in obesity and insulin resistance [J].Nat Rev Endocrinol,2012,8:709-716.  
[6] Kanter JE,Bornfeldt KE. Inflammation and diabetes-accelerated atherosclerosis:myeloid cell mediators[J].Trends Endocrinol Metab,2013,24(3):137-144.  
[7] Luotola K, Pietil?覿 A,Zeller T,et al.Associations between inter-leukin-1 (IL-1) gene variations or IL-1 receptor antagonist levels and the development of type 2 diabetes[J].Intern Med,2011,269(3): 322-332.  
[8] Vozarova B,Weyer C,Hanson K,et al.Circulating interleukin-6 in relation to adiposity,insulin action,and insulin secretion[J].Obes Res,2001,9(7):414-417.  
[9] Pradhan AD,Manson JE,Rifai N,et al.C-reactive protein,inter-leukin-6,and risk of developing type 2 diabetes mellitus [J].JAMA,2001,286(3):327-334.
[10] Ye J,McGuinness OP.Inflammation during obesity is not all bad:Evidence from animal and human studies[J].Physiol Endocrinol Metab,2013,304(5):E466-E477.
[11] Van Sickle BJ,Simmons J,Hall R,et al.Increased circulating IL-8 is associated with reduced IGF-1 and related to poor metabolic control in adolescents with type 1 diabetes mellitus.[J].Cytokine,2009,48(3):290-294.
[12] Koskela UE,Kuusisto SM,Nissinen AE,et al High vitreous concentration of IL-6 and IL-8,but not of adhesion molecules in relation to plasma concentrations in proliferative diabetic retinopathy [J].Ophthalmic Res,2013,49 (2):108-114.
[13] Fasshauer M,Paschke R.Regulation of adipocytokines and insulinresistance[J].Diabetologia,2003,46(12):1594-1603.
[14] Ruan H,Miles PD,Ladd CM,et al.Profiling gene transcription in vivo reveals adipose tissue as an immediate target of tumor necrosis factor-alpha:implications for insulin resistance[J].Diabetes,2002,51(11):3176-3188.
[15] Meng S,Wang LS,Huang ZQ,et al.Berberine ameliorates inflammation in patients with acute coronary syndrome following percutaneous coronary intervention[J].Clin Exp Pharmacol Physiol,2012,39(5):406-411.

相似文献/References:

[1]姚霜霜,张翼飞,张志国,等.小檗碱改善代谢性疾病的肠道相关机制[J].国际内分泌代谢杂志,2014,(06):386.[doi:10.3760/cma.j.issn.1673-4157.2014.06.007]
 Yao Shuangshuang,Zhang Yifei,Zhang Zhiguo,et al.The gut-related mechanisms of berberine in the treatment of metabolic diseases[J].International Journal of Endocrinology and Metabolism,2014,(04):386.[doi:10.3760/cma.j.issn.1673-4157.2014.06.007]

备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81373464,81270882);山西省自然科学基金资助项目(2013011047-3,2012011039-8);山西省卫生厅科研课题(201201062);山西省回国留学人员科研资助项目(2013-111);山西省留学人员择优启动项目(2011-762,2013-68);中华医学会临床医学科研专项资金资助项目(13040440429) 通信作者:杨静,Email:yangjlm@126.com
更新日期/Last Update: 2014-07-20