[1]王晓来,宋春青,邵海琳,等.体外培养高糖、高脂喂养大鼠主动脉平滑肌细胞钙化的研究[J].国际内分泌代谢杂志,2014,(03):145-148.[doi:10.3760/cma.j.issn.1673-4157.2014.03.001]
　Wang Xiaolai,Song Chunqing,Shao Hailin,et al.Calcification of aortic smooth muscle cells from high-sugar and high-fat diet rat cultured in vitro[J].International Journal of Endocrinology and Metabolism,2014,(03):145-148.[doi:10.3760/cma.j.issn.1673-4157.2014.03.001]
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体外培养高糖、高脂喂养大鼠主动脉平滑肌细胞钙化的研究()
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《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
期数:
2014年03期
页码:
145-148
栏目:
论著
出版日期:
2014-06-30

文章信息/Info

Title:
Calcification of aortic smooth muscle cells from high-sugar and high-fat diet rat cultured in vitro
作者:
王晓来; 宋春青; 邵海琳; 徐东红; 尚晓静; 郝兆虎
300140　天津第四中心医院内分泌科
Author(s):
Wang Xiaolai; Song Chunqing; Shao Hailin; Xu Donghong; Shang Xiaojing; Hao Zhaohu.
Department of Endocrinology,The Fourth Central Hospital of Tianjin,Tianjin 300140,China
关键词:
细胞钙化; 主动脉平滑肌细胞; Goto-Kakizaki大鼠; 糖尿病
Keywords:
Cell calcification; Aortic smooth muscle cells; Goto-Kakizaki rat; Diabetes mellitus
DOI:
10.3760/cma.j.issn.1673-4157.2014.03.001
摘要:
目的　检测高糖、高脂喂养Goto-Kakizaki（GK）糖尿病大鼠主动脉平滑肌细胞（SMCs）的血管钙化指标,探讨糖尿病血管钙化的相关机制。方法　高糖、高脂喂养GK及Wistar大鼠2周,同时分离培养两组大鼠的主动脉SMCs,Wistar大鼠SMCs作为对照。通过细胞计数法观察细胞生长状况,以甲基百里香酚蓝比色法测定两组大鼠细胞层及培养上清中钙的含量,实时定量PCR检测两组细胞碱性磷酸酶（ALP）、骨桥蛋白（OPN）、核心结合因子?琢-1（Cbfα-1）、?琢-平滑肌肌动蛋白（?琢-SMA）的基因表达。结果　与Wistar大鼠SMCs 相比,GK大鼠SMCs生长速度明显缓慢 （F =363.392,P ＜0.05）；细胞层钙含量[（0.56±0.22） vs. （0.39±0.09）,t = 2.47,P ＜0.05]明显增加,培养上清中钙含量[（0.82±0.22） vs.（1.20±0.17）,t = -22.573,P ＜0.05]明显减少。GK大鼠SMCs 中ALP （t = 12.963,P ＜0.05）、OPN （t = 8.305,P ＜0.05）及Cbf?琢-1（t = 10.109,P ＜0.05）的基因表达增加,同时?琢-SMA （t = -8.219, P ＜0.05）的基因表达减少。结论　高糖、高脂喂养的GK糖尿病大鼠的主动脉平滑肌细胞易发生钙化。
Abstract:
Objective　To investigate the mechanisms of diabetic vascular calcification by examining the markers related to vascular calcification of aortic smooth muscle cells（SMCs） isolated from the thoracic aortas of Goto-Kakizaki（GK） rats fed with high-sugar and high-fat diet. Methods　GK and Wistar rats were fed with high-sugar and high-fat diet for two weeks. SMCs were isolated from GK rats and Wistar rats simultaneously,and SMCs from Wistar rats were used as control.The growth of SMCs was observed by cell counting.The calcium contents in SMCs layer and supernatant were measured by methyl thymol blue method.Expression of alkaline phosphatase （ALP）,osteopontin （OPN）,core binding factor ?琢-1（ Cbf?琢-1）,?琢-smooth muscle actin （?琢-SMA） were detected by real-time quantitative PCR. Results　Compared with SMCs from Wistar rats, SMCs from GK rats growed slowly significantly （F =363.392,P ＜0.05）,the calcium contents was significantly increased in the cells' layer[（0.56±0.22） vs.（0.39±0.09）,t = 2.47,P ＜0.05], and was significantly reduced in the supernatant [（0.82±0.22） vs. （1.20±0.17）,t = -22.573,P ＜0.05] in GK rats group. The expression of ALP （t = 12.963,P ＜0.05）, OPN （t = 8.305,P ＜0.05）, Cbf?琢-1（t = 10.109,P ＜0.05） in SMCs from GK rats increased,while expression of ?琢-SMA decreased（t =-8.219,P ＜0.05）. Conclusions 　SMCs from high-sugar and high-fat fed GK diabetic rats tend to appear calcification.

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更新日期/Last Update: 2014-05-20