[1]刘国强,魏蕊,杨进,等.补充维生素D对中年雄性小鼠精子活力的影响[J].国际内分泌代谢杂志,2022,42(03):170-173,183.[doi:10.3760/cma.j.cn121383-20210206-02014]
　Liu Guoqiang,Wei Rui,Yang Jin,et al.Effects of vitamin D supplementation on sperm motility in middle aged male mice[J].International Journal of Endocrinology and Metabolism,2022,42(03):170-173,183.[doi:10.3760/cma.j.cn121383-20210206-02014]
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补充维生素D对中年雄性小鼠精子活力的影响()
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《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
42
期数:
2022年03期
页码:
170-173,183
栏目:
论著
出版日期:
2022-05-20

文章信息/Info

Title:
Effects of vitamin D supplementation on sperm motility in middle aged male mice
作者:
刘国强; 魏蕊; 杨进; 洪天配
北京大学第三医院内分泌科 100191
Author(s):
Liu Guoqiang;  Wei Rui;  Yang Jin;  Hong Tianpei.
Department of Endocrinology and Metabolism, Peking University Third Hospital, Beijing 100191, China
关键词:
维生素D;  精子活力;  增龄
Keywords:
Vitamin D;  Sperm motility;  Aging
DOI:
10.3760/cma.j.cn121383-20210206-02014
摘要:
目的 探讨补充维生素D(vitamin D,VD)对中年雄性小鼠精子活力的影响。方法 采用VD常规含量饲料喂养的7周龄雄性ICR小鼠随机分为4组:中年VD缺乏组、中年常规VD组、中年补充VD组、年轻常规VD组(每组7只),前3组小鼠8～28周龄分别给予VD缺乏、常规含量、高于常规含量的饲料(VD含量分别为<100、1 000及1 500 U/kg饲料)喂养,后一组小鼠持续采用VD常规含量的饲料喂养至8周龄。利用酶联免疫吸附测定(ELISA)法测定血清25-羟维生素D(25OHD)水平,采用计算机辅助精液分析系统检测精液各项参数。结果 中年补充VD组小鼠的血清25OHD水平显著高于其他3组小鼠,中年常规VD组和年轻常规VD组的血清25OHD水平显著高于中年VD缺乏组。各组间小鼠的睾丸和附睾的平均重量未见显著性差异。与中年常规VD组、中年VD缺乏组相比,中年补充VD组小鼠的精子活动率和前向运动速度显著升高,达到接近年轻常规VD组小鼠的水平。结论 补充VD有助于维持中年雄性小鼠的精子活力。
Abstract:
Objective To investigate the effect of vitamin D(VD)supplement on sperm motility in middle aged male mice.Methods Twenty-eight male ICR mice fed with regular VD diet aged 7 weeks, were randomized into four groups:middle aged VD-deficiency group, middle aged VD-regular group, middle aged VD-supplement group, and younger VD-regular group(n=7 in each group). Mice in the first three groups were fed with very low VD diet <100 U/kg, regular VD diet 1 000 U/kg, and high VD diet 1 500 U/kg, respectively from week 8th to week 28th, and the mice in younger VD-regular group were fed with regular VD diet up to week 8th. The level of 25-hydroxyvitamin D(25OHD)was determined by ELISA, and the semen parameters were determined by computer aided semen analysis system.Results The serum 25OHD level in the middle aged VD-supplement group was higher than that in the other three groups, and the levels of serum 25OHD in the middle aged and younger VD-regular groups were higher than that in the middle aged VD-deficiency group. There were no significant differences in the average weight of testis and epididymis among all groups. Compared with the middle aged VD-regular group and the middle aged VD-deficiency group, the sperm motility rate and straight-line velocity in the middle aged VD-supplement group were significantly elevated, approximate to the levels in younger VD-regular group.Conclusion VD supplement is helpful to maintain the sperm motility in middle aged male mice.

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备注/Memo

备注/Memo:
通信作者:魏蕊,Email:weirui@bjmu.edu.cn 基金项目:国家自然科学基金(81970617)
更新日期/Last Update: 2022-05-10