[1]柴锦燕,方佩华,谭建,等.定量检测人甲状腺过氧化物酶抗体 化学发光酶免疫分析法的临床应用[J].国际内分泌代谢杂志,2016,36(01):24-27.[doi:10.3760/cma.j.issn.1673-4157.2016.01.006]
　Chai Jinyan,Fang Peihua,Tan Jian,et al.Clinical application of chemiluminescence enzyme immunoassay to quantitatively detect human thyroid peroxidase antibodies[J].International Journal of Endocrinology and Metabolism,2016,36(01):24-27.[doi:10.3760/cma.j.issn.1673-4157.2016.01.006]
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定量检测人甲状腺过氧化物酶抗体 化学发光酶免疫分析法的临床应用()
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《国际内分泌代谢杂志》[ISSN:1673-4157/CN:12-1383/R]

卷:
36
期数:
2016年01期
页码:
24-27
栏目:
论著
出版日期:
2016-01-20

文章信息/Info

Title:
Clinical application of chemiluminescence enzyme immunoassay to quantitatively detect human thyroid peroxidase antibodies
作者:
柴锦燕; 方佩华; 谭建; 李宁
300052 天津医科大学总医院核医学科
Author(s):
Chai Jinyan;  Fang Peihua;  Tan Jian; Li Ning.
Department of Nuclear Medicine, The General Hospital of Tianjin Medical University, Tianjin 300052,China
关键词:
碘化物过氧化物酶;  化学发光酶免疫分析技术;  自身抗体;  桥本甲状腺炎
Keywords:
Iodide peroxidase;  Chemiluminescence enzyme immuneassay;  Autoantibodies;  Hashimoto's thyroiditis
DOI:
10.3760/cma.j.issn.1673-4157.2016.01.006
摘要:
目的 应用人甲状腺过氧化物酶抗体(hTPOAb)的化学发光酶免疫分析(CLEIA)试剂盒,检测正常人及各类甲状腺疾病患者,探讨该试剂盒在临床应用中的价值。方法 用该试剂盒共测定333例样品血清中的hTPOAb,包括正常人133名,各类甲状腺疾病患者200例,其中桥本甲状腺炎(HT)94例,Graves病68例,结节性甲状腺肿19例,亚急性甲状腺炎10例,单纯性甲状腺肿9例。结果 确立本试剂盒hTPOAb阳性切限值为3.22 IU/ml,hTPOAb浓度以中位数表示,分别为HT5.48 IU/ml,阳性率为91.50%; Graves病1.88 IU/ml,阳性率为29.40%; 结节性甲状腺肿1.83 IU/ml,阳性率为10.50%,亚急性甲状腺炎2.54 IU/ml,阳性率为20.00%; 单纯性甲状腺肿2.21 IU/ml,阳性率为0。HT患者血清hTPOAb阳性率高于其他患者(χ2=67.22,60.13,35.49,49.89,P均<0.01)。结论 该定量检测hTPOAb的CLEIA试剂盒在HT中有很高的阳性率,可作为HT有效的诊断手段。
Abstract:
Objective To apply chemiluminescence enzyme immunoassay(CLEIA)kits of thyroid peroxidase antibodies(hTPOAb)on normal and all kinds of patients with thyroid diseases, and explore the clinical value of the kits.Methods The hTPOAb kits were used in 333 cases of serum samples, including 133 cases of healthy people, 94 cases of Hashimoto's thyroiditis(HT),68 cases of Graves' disease(GD), 19 cases of nodular goiter,10 cases of subacute thyroiditis and 9 cases of simple goiter. Results Concentration above 3.22 IU/ml was considered to be positive of the hTPOAb kits. The median concentration of hTPOAb was 5.48 IU/ml in HT(positive ratio was 91.50%),1.88 IU/ml in GD(positive ratio was 29.40%),1.83 IU/ml in nodular goiter(positive ratio was 10.50%),2.54 IU/ml in subacute thyroiditis(positive ratio was 20.00%),and 2.21 IU/ml in simple goiter(positive ratio was 0).The positive ratio of hTPOAb in patients with HT was higher than that of the other patients(χ2=67.22,60.13,35.49,49.89,all P<0.01). Conclusion The CLEIA kits of hTPOAb has a high positive rate for HT, and is an effective method in diagnosing HT.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:国家临床重点专科建设项目
更新日期/Last Update: 2016-01-20